{"entity": "researcher", "timestamp": "2026-06-08T05:43:00.727Z", "family": "Condello", "given": "Vincenzo", "initials": "V", "orcid": "0000-0003-4569-5398", "affiliations": ["Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden. vincenzo.condello@ki.se."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/09be613118f743bd992bba237b61ceb4.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/09be613118f743bd992bba237b61ceb4"}}, "publications": [{"entity": "publication", "iuid": "e09133a40d7145b18f9bb8facb4dc96c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e09133a40d7145b18f9bb8facb4dc96c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e09133a40d7145b18f9bb8facb4dc96c"}}, "title": "Comprehensive Gene Expression Analysis in Papillary Thyroid Carcinoma Reveals a Transcriptional Profile Associated with Reduced Radioiodine Avidity.", "authors": [{"family": "Condello", "given": "Vincenzo", "initials": "V", "orcid": "0000-0003-4569-5398", "researcher": {"href": "https://publications.scilifelab.se/researcher/09be613118f743bd992bba237b61ceb4.json"}}, {"family": "Marchettini", "given": "Carlotta", "initials": "C"}, {"family": "Ihre-Lundgren", "given": "Catharina", "initials": "C"}, {"family": "Nilsson", "given": "Joachim N", "initials": "JN", "orcid": "0000-0001-7496-9189", "researcher": {"href": "https://publications.scilifelab.se/researcher/47b5460e0a2444c49675506d7f028784.json"}}, {"family": "Juhlin", "given": "C Christofer", "initials": "CC", "orcid": "0000-0002-5945-9081", "researcher": {"href": "https://publications.scilifelab.se/researcher/bb660e24421749d4acaaf6e9a90042f8.json"}}], "type": "journal article", "published": "2025-02-21", "journal": {"title": "Endocr. Pathol.", "issn": "1559-0097", "volume": "36", "issue": "1", "pages": "4", "issn-l": "1046-3976"}, "abstract": "Papillary thyroid carcinoma (PTC) is the most common form of well-differentiated thyroid cancer (WDTC) and generally has a favorable prognosis. However, subsets of these tumors can metastasize, leading to aggressive disease progression and poorer clinical outcomes. Radioactive iodine (RAI) therapy is routinely given in the adjuvant setting following thyroidectomy and lymph node dissection for WDTC. Nevertheless, its therapeutic efficacy is limited to tumors with high iodine avidity. Early post-surgical classification of thyroid cancers as either iodine-avid or refractory is crucial for enabling more personalized and effective treatment strategies. In this study, we aimed to identify transcriptomic determinants associated with RAI refractoriness (RAI-R) to improve prognostication. We collected clinicopathologic data and conducted RNA-seq on 36 tissue samples (18 high-avidity and 18 low-avidity), each uniquely characterized by ex vivo iodine concentration measurements taken directly from surgical specimens. Whole-transcriptomic analysis identified 63 differentially expressed genes, with six (S100A4, CRTC2, ANO1, WWTR1, DEPTOR, MT1G) showing consistent deregulation. The expression of ANO1, an established iodine transporter at the apical membrane of the thyroid follicular cells, correlated significantly with iodine avidity (r = 0.54). Validation via RT-qPCR confirmed differential expression trends. Gene ontology and pathway enrichment analyses highlighted thyroid hormone synthesis, PI3K-AKT, and MAPK signaling pathways as key regulators of RAI avidity. A refined multivariate predictive model incorporating ANO1 mRNA expression, histological subtypes, and sample type demonstrated strong predictive performance (adjusted R2 = 0.55). These findings suggest ANO1 as a promising biomarker for predicting iodine avidity in thyroid cancer.", "doi": "10.1007/s12022-025-09849-0", "pmid": "39982585", "labels": {"NGI Short read": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11845550"}, {"db": "pii", "key": "10.1007/s12022-025-09849-0"}], "notes": [], "created": "2025-05-12T05:46:47.965Z", "modified": "2025-09-08T07:13:03.100Z"}, {"entity": "publication", "iuid": "df8fde700de34a3aaee98c266abd7265", "links": {"self": {"href": "https://publications.scilifelab.se/publication/df8fde700de34a3aaee98c266abd7265.json"}, "display": {"href": "https://publications.scilifelab.se/publication/df8fde700de34a3aaee98c266abd7265"}}, "title": "Spatial Transcriptomics in a Case of Follicular Thyroid Carcinoma Reveals Clone-Specific Dysregulation of Genes Regulating Extracellular Matrix in the Invading Front.", "authors": [{"family": "Condello", "given": "Vincenzo", "initials": "V", "orcid": "0000-0003-4569-5398", "researcher": {"href": "https://publications.scilifelab.se/researcher/09be613118f743bd992bba237b61ceb4.json"}}, {"family": "Paulsson", "given": "Johan O", "initials": "JO", "orcid": "0000-0003-0390-6740", "researcher": {"href": "https://publications.scilifelab.se/researcher/762d22bad5b7462dbba2a87c9b3221a0.json"}}, {"family": "Zedenius", "given": "Jan", "initials": "J", "orcid": "0000-0003-2833-3758", "researcher": {"href": "https://publications.scilifelab.se/researcher/319fe27b84674ea3829d2e0c0e0ab966.json"}}, {"family": "N\u00e4sman", "given": "Anders", "initials": "A", "orcid": "0000-0003-4602-4297", "researcher": {"href": "https://publications.scilifelab.se/researcher/368352486dc54915b6873ad1aae59ea2.json"}}, {"family": "Juhlin", "given": "C Christofer", "initials": "CC", "orcid": "0000-0002-5945-9081", "researcher": {"href": "https://publications.scilifelab.se/researcher/bb660e24421749d4acaaf6e9a90042f8.json"}}], "type": "journal article", "published": "2024-06-00", "journal": {"title": "Endocr. Pathol.", "issn": "1559-0097", "volume": "35", "issue": "2", "pages": "122-133", "issn-l": "1046-3976"}, "abstract": "Follicular thyroid carcinoma (FTC) is recognized by its ability to invade the tumor capsule and blood vessels, although the exact molecular signals orchestrating this phenotype remain elusive. In this study, the spatial transcriptional landscape of an FTC is detailed with comparisons between the invasive front and histologically indolent central core tumor areas. The Visium spatial gene expression platform allowed us to interrogate and visualize the whole transcriptome in 2D across formalin-fixated paraffin-embedded (FFPE) tissue sections. Four different 6 \u00d7 6 mm areas of an FTC were scrutinized, including regions with capsular and vascular invasion, capsule-near area without invasion, and a central core area of the tumor. Following successful capturing and sequencing, several expressional clusters were identified with regional variation. Most notably, invasive tumor cell clusters were significantly over-expressing genes associated with pathways interacting with the extracellular matrix (ECM) remodeling and epithelial-to-mesenchymal transition (EMT). Subsets of these genes (POSTN and DPYSL3) were additionally validated using immunohistochemistry in an independent cohort of follicular thyroid tumors showing a clear gradient pattern from the core to the periphery of the tumor. Moreover, the reconstruction of the evolutionary tree identified the invasive clones as late events in follicular thyroid tumorigenesis. To our knowledge, this is one of the first 2D global transcriptional mappings of FTC using this platform to date. Invasive FTC clones develop in a stepwise fashion and display significant dysregulation of genes associated with the ECM and EMT - thus highlighting important molecular crosstalk for further investigations.", "doi": "10.1007/s12022-024-09798-0", "pmid": "38280140", "labels": {"NGI Short read": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Stockholm (Genomics Applications)": "Service", "NGI Spatial omics": "Service", "National Genomics Infrastructure": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11176252"}, {"db": "pii", "key": "10.1007/s12022-024-09798-0"}], "notes": [], "created": "2024-02-13T07:46:50.221Z", "modified": "2024-11-25T10:11:30.955Z"}]}