{"entity": "researcher", "timestamp": "2026-06-15T17:59:13.960Z", "family": "Collani", "given": "Silvio", "initials": "S", "orcid": "0000-0002-9603-0882", "affiliations": ["1Ume\u00e5 Plant Science Centre, Department of Plant Physiology, Ume\u00e5 University (Umu), Ume\u00e5, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/0896530185b54e16824cd26658ccdfc8.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/0896530185b54e16824cd26658ccdfc8"}}, "publications": [{"entity": "publication", "iuid": "eede36ef32fd4a6aac7dd2a2b9baa667", "links": {"self": {"href": "https://publications.scilifelab.se/publication/eede36ef32fd4a6aac7dd2a2b9baa667.json"}, "display": {"href": "https://publications.scilifelab.se/publication/eede36ef32fd4a6aac7dd2a2b9baa667"}}, "title": "The splicing genes SmEa and SmEb regulate plant development during vegetative growth in poplar.", "authors": [{"family": "Goretti", "given": "Daniela", "initials": "D", "orcid": "0000-0003-3996-0204", "researcher": {"href": "https://publications.scilifelab.se/researcher/cabe11cdf08446bda52aa5d2b62ea239.json"}}, {"family": "Collani", "given": "Silvio", "initials": "S", "orcid": "0000-0002-9603-0882", "researcher": {"href": "https://publications.scilifelab.se/researcher/0896530185b54e16824cd26658ccdfc8.json"}}, {"family": "Marcon", "given": "Alice", "initials": "A", "orcid": "0009-0006-9957-6115", "researcher": {"href": "https://publications.scilifelab.se/researcher/9fb0b7d67e464160bda5a6acfdf96d95.json"}}, {"family": "Nilsson", "given": "Ove", "initials": "O", "orcid": "0000-0002-1033-1909", "researcher": {"href": "https://publications.scilifelab.se/researcher/729146afe5e24eb0a6f107db10e95e01.json"}}, {"family": "Schmid", "given": "Markus", "initials": "M", "orcid": "0000-0002-0068-2967", "researcher": {"href": "https://publications.scilifelab.se/researcher/8705d242aa8f4f92b930c2cdc23254f0.json"}}], "type": "journal article", "published": "2025-12-23", "journal": {"title": "BMC Plant Biol.", "issn": "1471-2229", "volume": "25", "issue": "1", "pages": "1723", "issn-l": "1471-2229"}, "abstract": "Spliceosomes are large evolutionary conserved ribonucleoprotein complexes containing at their core heptameric rings of Sm (or LSm) proteins and U-rich snRNAs. The role of Sm proteins in animal development is well established, and recent research has begun to link mutations in these genes to growth defects in plants. One of the most studied Sm genes is SmE1/PCP, mutants of which display a temperature-dependent phenotype in Arabidopsis thaliana.\n\nThis study provides a first glimpse into the function of a core splicing protein in the regulation of growth in a perennial species. Phylogenetic analysis identified two paralogous SmE genes in poplar, named SmEa and SmEb, that encode identical proteins and are orthologs of SmEs from Arabidopsis, as suggested by Y2H and in vivo experiments. CRISPR/Cas9 mutagenesis in hybrid aspen identified a role for SmEs in development in plants grown in an environment simulating seasonal photoperiod and temperature changes. Unlike in Arabidopsis, low temperatures had no or only a very minor effect on the development of sme mutants in aspen.\n\nWe identified specific aspects of SmE in poplar, highlighting the importance of examining the physiological and evolutionary differences that define this gene family in woody compared to herbaceous plants.\n\nThe online version contains supplementary material available at 10.1186/s12870-025-07676-3.", "doi": "10.1186/s12870-025-07676-3", "pmid": "41436944", "labels": {"National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service", "NGI Short read": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12729064"}, {"db": "pii", "key": "10.1186/s12870-025-07676-3"}], "notes": [], "created": "2026-01-07T11:04:57.648Z", "modified": "2026-01-07T11:04:57.822Z"}, {"entity": "publication", "iuid": "74c38ce26afa4ab181be8918dda27738", "links": {"self": {"href": "https://publications.scilifelab.se/publication/74c38ce26afa4ab181be8918dda27738.json"}, "display": {"href": "https://publications.scilifelab.se/publication/74c38ce26afa4ab181be8918dda27738"}}, "title": "A bacterial assay for rapid screening of IAA catabolic enzymes.", "authors": [{"family": "Brunoni", "given": "Federica", "initials": "F", "orcid": "0000-0003-1497-9419", "researcher": {"href": "https://publications.scilifelab.se/researcher/8a28132c7a0e407da31e4d79698679c6.json"}}, {"family": "Collani", "given": "Silvio", "initials": "S", "orcid": "0000-0002-9603-0882", "researcher": {"href": "https://publications.scilifelab.se/researcher/0896530185b54e16824cd26658ccdfc8.json"}}, {"family": "\u0160imura", "given": "Jan", "initials": "J", "orcid": "0000-0002-1567-2278", "researcher": {"href": "https://publications.scilifelab.se/researcher/ac1674ad68434ab19f17056e7824c932.json"}}, {"family": "Schmid", "given": "Markus", "initials": "M", "orcid": "0000-0002-0068-2967", "researcher": {"href": "https://publications.scilifelab.se/researcher/8705d242aa8f4f92b930c2cdc23254f0.json"}}, {"family": "Bellini", "given": "Catherine", "initials": "C", "orcid": "0000-0003-2985-6649", "researcher": {"href": "https://publications.scilifelab.se/researcher/644f290e267f43fa9c5e76b2f159a62d.json"}}, {"family": "Ljung", "given": "Karin", "initials": "K", "orcid": "0000-0003-2901-189X", "researcher": {"href": "https://publications.scilifelab.se/researcher/f91b1e1f90c24559b915ebcd265804a4.json"}}], "type": "journal article", "published": "2019-11-04", "journal": {"title": "Plant Methods", "issn": "1746-4811", "volume": "15", "issue": "1", "pages": "126", "issn-l": "1746-4811"}, "abstract": "Plants rely on concentration gradients of the native auxin, indole-3-acetic acid (IAA), to modulate plant growth and development. Both metabolic and transport processes participate in the dynamic regulation of IAA homeostasis. Free IAA levels can be reduced by inactivation mechanisms, such as conjugation and degradation. IAA can be conjugated via ester linkage to glucose, or via amide linkage to amino acids, and degraded via oxidation. Members of the UDP glucosyl transferase (UGT) family catalyze the conversion of IAA to indole-3-acetyl-1-glucosyl ester (IAGlc); by contrast, IAA is irreversibly converted to indole-3-acetyl-l-aspartic acid (IAAsp) and indole-3-acetyl glutamic acid (IAGlu) by Group II of the GRETCHEN HAGEN3 (GH3) family of acyl amido synthetases. Dioxygenase for auxin oxidation (DAO) irreversibly oxidizes IAA to oxindole-3-acetic acid (oxIAA) and, in turn, oxIAA can be further glucosylated to oxindole-3-acetyl-1-glucosyl ester (oxIAGlc) by UGTs. These metabolic pathways have been identified based on mutant analyses, in vitro activity measurements, and in planta feeding assays. In vitro assays for studying protein activity are based on producing Arabidopsis enzymes in a recombinant form in bacteria or yeast followed by recombinant protein purification. However, the need to extract and purify the recombinant proteins represents a major obstacle when performing in vitro assays.\n\nIn this work we report a rapid, reproducible and cheap method to screen the enzymatic activity of recombinant proteins that are known to inactivate IAA. The enzymatic reactions are carried out directly in bacteria that produce the recombinant protein. The enzymatic products can be measured by direct injection of a small supernatant fraction from the bacterial culture on ultrahigh-performance liquid chromatography coupled to electrospray ionization tandem spectrometry (UHPLC-ESI-MS/MS). Experimental procedures were optimized for testing the activity of different classes of IAA-modifying enzymes without the need to purify recombinant protein.\n\nThis new method represents an alternative to existing in vitro assays. It can be applied to the analysis of IAA metabolites that are produced upon supplementation of substrate to engineered bacterial cultures and can be used for a rapid screening of orthologous candidate genes from non-model species.", "doi": "10.1186/s13007-019-0509-6", "pmid": "31700527", "labels": {"Swedish Metabolomics Centre": "Service"}, "xrefs": [{"db": "pii", "key": "509"}, {"db": "pmc", "key": "PMC6827244"}], "notes": [], "created": "2020-01-07T15:51:28.889Z", "modified": "2025-10-17T13:03:17.248Z"}]}