{"entity": "researcher", "timestamp": "2026-03-15T17:22:17.817Z", "family": "Jin", "given": "Tao", "initials": "T", "orcid": "0000-0001-9039-0628", "affiliations": ["Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, 41390 Gothenburg, Sweden.", "Department of Rheumatology, Sahlgrenska University Hospital, 41345 Gothenburg, Sweden."], "links": {"self": {"href": "https://publications.scilifelab.se/researcher/03ca682de4fe4a7eb667dc63cae88335.json"}, "display": {"href": "https://publications.scilifelab.se/researcher/03ca682de4fe4a7eb667dc63cae88335"}}, "publications": [{"entity": "publication", "iuid": "55595ac6dbaf426ebbbaa4d12d7c4901", "links": {"self": {"href": "https://publications.scilifelab.se/publication/55595ac6dbaf426ebbbaa4d12d7c4901.json"}, "display": {"href": "https://publications.scilifelab.se/publication/55595ac6dbaf426ebbbaa4d12d7c4901"}}, "title": "Surface-Shaving of Staphylococcus aureus Strains and Quantitative Proteomic Analysis Reveal Differences in Protein Abundance of the Surfaceome.", "authors": [{"family": "Karlsson", "given": "Anders", "initials": "A"}, {"family": "Alarc\u00f3n", "given": "Leonarda Ach\u00e1", "initials": "LA", "orcid": "0000-0002-9453-7479", "researcher": {"href": "https://publications.scilifelab.se/researcher/6fa254340a5b4ec39809654b54fd3481.json"}}, {"family": "Pi\u00f1eiro-Iglesias", "given": "Beatriz", "initials": "B"}, {"family": "Jacobsson", "given": "Gunnar", "initials": "G"}, {"family": "Skovbjerg", "given": "Susann", "initials": "S"}, {"family": "Moore", "given": "Edward R B", "initials": "ERB", "orcid": "0000-0001-7693-924X", "researcher": {"href": "https://publications.scilifelab.se/researcher/10b1c68436094391bfefd7de22757aba.json"}}, {"family": "Kopparapu", "given": "Pradeep Kumar", "initials": "PK", "orcid": "0000-0003-3941-748X", "researcher": {"href": "https://publications.scilifelab.se/researcher/8d0d9c2301c8413899a867ace6877cb2.json"}}, {"family": "Jin", "given": "Tao", "initials": "T", "orcid": "0000-0001-9039-0628", "researcher": {"href": "https://publications.scilifelab.se/researcher/03ca682de4fe4a7eb667dc63cae88335.json"}}, {"family": "Karlsson", "given": "Roger", "initials": "R", "orcid": "0000-0002-5919-2639", "researcher": {"href": "https://publications.scilifelab.se/researcher/bd9b10fd0fa34dd9a3de96f3c4860e32.json"}}], "type": "journal article", "published": "2024-08-21", "journal": {"title": "Microorganisms", "issn": "2076-2607", "volume": "12", "issue": "8", "issn-l": "2076-2607"}, "abstract": "Staphylococcus aureus is a pathogen known to cause a wide range of infections. To find new targets for identification and to understand host-pathogen interactions, many studies have focused on surface proteins. We performed bacterial-cell surface-shaving, followed by tandem mass tag for quantitative mass spectrometry proteomics, to examine the surfaceome of S. aureus. Two steps were performed, the first step including surface protein-deficient mutants of S. aureus Newman strain lacking important virulence genes (clfA and spa, important for adhesion and immune evasion and srtAsrtB, linking surface-associated virulence factors to the surface) and the second step including isolates of different clinical origin. All strains were compared to the Newman strain. In Step 1, altogether, 7880 peptides were identified, corresponding to 1290 proteins. In Step 2, 4949 peptides were identified, corresponding to 919 proteins and for each strain, approximately 20 proteins showed differential expression compared to the Newman strain. The identified surface proteins were related to host-cell-adherence and immune-system-evasion, biofilm formation, and survival under harsh conditions. The results indicate that surface-shaving of intact S. aureus bacterial strains in combination with quantitative proteomics is a useful tool to distinguish differences in protein abundance of the surfaceome, including the expression of virulence factors.", "doi": "10.3390/microorganisms12081725", "pmid": "39203567", "labels": {"Glycoproteomics and MS Proteomics": "Service", "Clinical Genomics Gothenburg": "Service", "Clinical Genomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11357550"}, {"db": "pii", "key": "microorganisms12081725"}], "notes": [], "created": "2024-09-15T11:45:41.565Z", "modified": "2024-11-27T16:31:45.417Z"}, {"entity": "publication", "iuid": "9192b9790f6b4cd5b16bdc78b2e02338", "links": {"self": {"href": "https://publications.scilifelab.se/publication/9192b9790f6b4cd5b16bdc78b2e02338.json"}, "display": {"href": "https://publications.scilifelab.se/publication/9192b9790f6b4cd5b16bdc78b2e02338"}}, "title": "Local Immune Activation and Age Impact on Humoral Immunity in Mice, with a Focus on IgG Sialylation.", "authors": [{"family": "Gupta", "given": "Priti", "initials": "P"}, {"family": "S\u00e1ghy", "given": "Tibor", "initials": "T", "orcid": "0000-0002-5835-3912", "researcher": {"href": "https://publications.scilifelab.se/researcher/20edaf4a38234ccab1aed842f7f6ac90.json"}}, {"family": "Bollmann", "given": "Miriam", "initials": "M", "orcid": "0000-0003-1639-8079", "researcher": {"href": "https://publications.scilifelab.se/researcher/200b2c01c5644aae924517e456730c4f.json"}}, {"family": "Jin", "given": "Tao", "initials": "T", "orcid": "0000-0001-9039-0628", "researcher": {"href": "https://publications.scilifelab.se/researcher/03ca682de4fe4a7eb667dc63cae88335.json"}}, {"family": "Ohlsson", "given": "Claes", "initials": "C"}, {"family": "Carlsten", "given": "Hans", "initials": "H"}, {"family": "Corciulo", "given": "Carmen", "initials": "C", "orcid": "0000-0002-1865-3494", "researcher": {"href": "https://publications.scilifelab.se/researcher/ab4485384ad1401780c1aa6bb208041a.json"}}, {"family": "Engdahl", "given": "Cecilia", "initials": "C", "orcid": "0000-0002-8914-0178", "researcher": {"href": "https://publications.scilifelab.se/researcher/e5fc234e678947849cb9c790767d14b3.json"}}], "type": "journal article", "published": "2024-04-29", "journal": {"title": "Vaccines (Basel)", "issn": "2076-393X", "volume": "12", "issue": "5", "issn-l": null}, "abstract": "Age alters the host's susceptibility to immune induction. Humoral immunity with circulating antibodies, particularly immunoglobulin G (IgG), plays an essential role in immune response. IgG glycosylation in the fragment crystallizable (Fc) region, including sialylation, is important in regulating the effector function by interacting with Fc gamma receptors (Fc\u03b3Rs). Glycosylation is fundamentally changed with age and inflammatory responses. We aimed to explore the regulation of humoral immunity by comparing responses to antigen-induced immune challenges in young and adult mice using a local antigen-induced arthritis mouse model. This study examines the differences in immune response between healthy and immune-challenged states across these groups. Our initial assessment of the arthritis model indicated that adult mice presented more severe knee swelling than their younger counterparts. In contrast, we found that neither histological assessment, bone mineral density, nor the number of osteoclasts differs. Our data revealed an age-associated but not immune challenge increase in total IgG; the only subtype affected by immune challenge was IgG1 and partially IgG3. Interestingly, the sialylation of IgG2b and IgG3 is affected by age and immune challenges but not stimulated further by immune challenges in adult mice. This suggests a shift in IgG towards a pro-inflammatory and potentially pathogenic state with age and inflammation.", "doi": "10.3390/vaccines12050479", "pmid": "38793730", "labels": {"Glycoproteomics and MS Proteomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11125885"}, {"db": "pii", "key": "vaccines12050479"}], "notes": [], "created": "2024-11-27T15:35:54.028Z", "modified": "2024-11-27T15:35:54.296Z"}]}