{"entity": "publication", "iuid": "fe11d7e323b2447091d2e5073a72931f", "timestamp": "2026-07-22T13:25:06.161Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/fe11d7e323b2447091d2e5073a72931f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/fe11d7e323b2447091d2e5073a72931f"}}, "title": "Breast cancer patient-derived scaffolds enhance the understanding of PD-L1 regulation and T cell cytotoxicity.", "authors": [{"family": "Garre", "given": "Elena", "initials": "E", "orcid": "0000-0002-3827-4977", "researcher": {"href": "https://publications.scilifelab.se/researcher/90df2c41baee4b0c98035fbc5f77a438.json"}}, {"family": "Rhost", "given": "Sara", "initials": "S"}, {"family": "Gustafsson", "given": "Anna", "initials": "A"}, {"family": "Szeponik", "given": "Louis", "initials": "L", "orcid": "0000-0002-8627-6969", "researcher": {"href": "https://publications.scilifelab.se/researcher/ca113a84f7d14ae89fc0165885e21972.json"}}, {"family": "Araujo", "given": "Thais Fenz", "initials": "TF"}, {"family": "Quiding-J\u00e4rbrink", "given": "Marianne", "initials": "M"}, {"family": "Helou", "given": "Khalil", "initials": "K"}, {"family": "St\u00e5hlberg", "given": "Anders", "initials": "A", "orcid": "0000-0003-4243-0191", "researcher": {"href": "https://publications.scilifelab.se/researcher/05306b130d6543eea88a4f518085981e.json"}}, {"family": "Landberg", "given": "G\u00f6ran", "initials": "G", "orcid": "0000-0001-9004-9403", "researcher": {"href": "https://publications.scilifelab.se/researcher/aa3b79caacba4566897f136a1200476f.json"}}], "type": "journal article", "published": "2025-04-16", "journal": {"title": "Commun Biol", "issn": "2399-3642", "volume": "8", "issue": "1", "pages": "621", "issn-l": "2399-3642"}, "abstract": "Recent advances as well as obstacles for immune-based cancer treatment strategies, highlight the notable impact of patient cancer microenvironments on the immune cells and immune targets. Here, we use patient-derived scaffolds (PDS) generated from 110 primary breast cancers to monitor the impact of the cancer microenvironment on immune regulators. Pronounced variation in PD-L1 expression is observed in cancer cells adapted to different patient scaffolds. This variation is further linked to clinical observations and correlated with specific proteins detected in the cell-free PDSs using mass spectrometry. When adding T cells to the PDS-based cancer cultures, the killing efficiency of activated T cells vary between the cultures, whereas non-activated T cells modulate the cancer cell PD-L1 expression to treatment-predictive values, matching killing capacities of activated T cells. Surviving cancer cells show enrichment in cancer stem cell and epithelial-to-mesenchymal transition (EMT) features, suggesting that T cells may not efficiently target cells with metastatic potential. We conclude that clinically relevant insights in how to optimally target and guide immune-based cancer therapies can be obtained by including patient-derived scaffolds and cues from the cancer microenvironment in cancer patient handling and drug development.", "doi": "10.1038/s42003-025-08054-3", "pmid": "40240529", "labels": {"Glycoproteomics and MS Proteomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12003762"}, {"db": "pii", "key": "10.1038/s42003-025-08054-3"}], "notes": [], "created": "2025-10-23T16:17:59.440Z", "modified": "2025-10-23T16:17:59.771Z"}