{"entity": "publication", "iuid": "fd5339728a92423293ad65afe5cbdb19", "timestamp": "2026-07-22T14:39:48.089Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/fd5339728a92423293ad65afe5cbdb19.json"}, "display": {"href": "https://publications.scilifelab.se/publication/fd5339728a92423293ad65afe5cbdb19"}}, "title": "Evolutionary conservation of the ribosomal biogenesis factor Rbm19/Mrd1: implications for function.", "authors": [{"family": "Kallberg", "given": "Yvonne", "initials": "Y"}, {"family": "Segerstolpe", "given": "\u00c5sa", "initials": "\u00c5"}, {"family": "Lackmann", "given": "Fredrik", "initials": "F"}, {"family": "Persson", "given": "Bengt", "initials": "B", "orcid": "0000-0003-3165-5344", "researcher": {"href": "https://publications.scilifelab.se/researcher/38f116ef0ed146419cb18e742c270c4a.json"}}, {"family": "Wieslander", "given": "Lars", "initials": "L"}], "type": "journal article", "published": "2012-09-12", "journal": {"volume": "7", "issn": "1932-6203", "issue": "9", "pages": "e43786", "title": "PLoS ONE", "issn-l": "1932-6203"}, "abstract": "Ribosome biogenesis in eukaryotes requires coordinated folding and assembly of a pre-rRNA into sequential pre-rRNA-protein complexes in which chemical modifications and RNA cleavages occur. These processes require many small nucleolar RNAs (snoRNAs) and proteins. Rbm19/Mrd1 is one such protein that is built from multiple RNA-binding domains (RBDs). We find that Rbm19/Mrd1 with five RBDs is present in all branches of the eukaryotic phylogenetic tree, except in animals and Choanoflagellates, that instead have a version with six RBDs and Microsporidia which have a minimal Rbm19/Mrd1 protein with four RBDs. Rbm19/Mrd1 therefore evolved as a multi-RBD protein very early in eukaryotes. The linkers between the RBDs have conserved properties; they are disordered, except for linker 3, and position the RBDs at conserved relative distances from each other. All but one of the RBDs have conserved properties for RNA-binding and each RBD has a specific consensus sequence and a conserved position in the protein, suggesting a functionally important modular design. The patterns of evolutionary conservation provide information for experimental analyses of the function of Rbm19/Mrd1. In vivo mutational analysis confirmed that a highly conserved loop 5-\u03b24-strand in RBD6 is essential for function.", "doi": "10.1371/journal.pone.0043786", "pmid": "22984444", "labels": {"Bioinformatics Support, Infrastructure and Training": null, "Bioinformatics Support and Infrastructure": null, "Bioinformatics (NBIS)": null}, "xrefs": [{"db": "pii", "key": "PONE-D-12-14217"}, {"db": "pmc", "key": "PMC3440411"}], "notes": [], "created": "2017-05-04T14:56:19.726Z", "modified": "2021-07-05T12:34:46.059Z"}