{"entity": "publication", "iuid": "f5ae0bcb00e84e21ab0bdf4d81044a0c", "timestamp": "2026-08-10T14:14:30.297Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f5ae0bcb00e84e21ab0bdf4d81044a0c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f5ae0bcb00e84e21ab0bdf4d81044a0c"}}, "title": "HiRIEF LC-MS enables deep proteome coverage and unbiased proteogenomics.", "authors": [{"family": "Branca", "given": "Rui M M", "initials": "RM", "orcid": "0000-0003-3890-6476", "researcher": {"href": "https://publications.scilifelab.se/researcher/87d6256540174d3da581d4572f9d182a.json"}}, {"family": "Orre", "given": "Lukas M", "initials": "LM", "orcid": "0000-0002-0384-1003", "researcher": {"href": "https://publications.scilifelab.se/researcher/7b4e49a93b0143db88059c4d1e9fdc59.json"}}, {"family": "Johansson", "given": "Henrik J", "initials": "HJ", "orcid": "0000-0003-4729-4205", "researcher": {"href": "https://publications.scilifelab.se/researcher/18aebf211fa640f48a7c8d860c168e5a.json"}}, {"family": "Granholm", "given": "Viktor", "initials": "V"}, {"family": "Huss", "given": "Mikael", "initials": "M"}, {"family": "P\u00e9rez-Bercoff", "given": "\u00c5sa", "initials": "\u00c5"}, {"family": "Forshed", "given": "Jenny", "initials": "J"}, {"family": "K\u00e4ll", "given": "Lukas", "initials": "L", "orcid": "0000-0001-5689-9797", "researcher": {"href": "https://publications.scilifelab.se/researcher/9c9f4444f83e43d79c4772a430ca3969.json"}}, {"family": "Lehti\u00f6", "given": "Janne", "initials": "J", "orcid": "0000-0002-8100-9562", "researcher": {"href": "https://publications.scilifelab.se/researcher/8406a97bac744a59b1bc951978994581.json"}}], "type": "journal article", "published": "2014-01-00", "journal": {"title": "Nat. Methods", "issn": "1548-7105", "volume": "11", "issue": "1", "pages": "59-62", "issn-l": "1548-7091"}, "abstract": "We present a liquid chromatography-mass spectrometry (LC-MS)-based method permitting unbiased (gene prediction-independent) genome-wide discovery of protein-coding loci in higher eukaryotes. Using high-resolution isoelectric focusing (HiRIEF) at the peptide level in the 3.7-5.0 pH range and accurate peptide isoelectric point (pI) prediction, we probed the six-reading-frame translation of the human and mouse genomes and identified 98 and 52 previously undiscovered protein-coding loci, respectively. The method also enabled deep proteome coverage, identifying 13,078 human and 10,637 mouse proteins.", "doi": "10.1038/nmeth.2732", "pmid": "24240322", "labels": {"National Genomics Infrastructure": "Service", "Bioinformatics Support, Infrastructure and Training": "Service", "NGI Stockholm (Genomics Applications)": "Service", "NGI Stockholm (Genomics Production)": "Service", "Bioinformatics Support and Infrastructure": "Service", "Bioinformatics (NBIS)": "Service"}, "xrefs": [{"db": "pii", "key": "nmeth.2732"}], "notes": [], "created": "2023-06-16T12:47:04.864Z", "modified": "2023-06-16T12:47:27.234Z"}