{"entity": "publication", "iuid": "f2b37f297e674bd59ee4fa8b88976193", "timestamp": "2026-08-12T06:06:07.355Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f2b37f297e674bd59ee4fa8b88976193.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f2b37f297e674bd59ee4fa8b88976193"}}, "title": "miR-20b regulates expression of proteinase-activated receptor-1 (PAR-1) thrombin receptor in melanoma cells.", "authors": [{"family": "Saleiban", "given": "Amina", "initials": "A"}, {"family": "Fax\u00e4lv", "given": "Lars", "initials": "L"}, {"family": "Claesson", "given": "Kjersti", "initials": "K"}, {"family": "J\u00f6nsson", "given": "Jan-Ingvar", "initials": "JI", "orcid": "0000-0003-4814-978X", "researcher": {"href": "https://publications.scilifelab.se/researcher/fcc87190783c48229913c2a35a2782c8.json"}}, {"family": "Osman", "given": "Abdimajid", "initials": "A"}], "type": "journal article", "published": "2014-05-00", "journal": {"volume": "27", "issn": "1755-148X", "issue": "3", "pages": "431-441", "title": "Pigment Cell Melanoma Res", "issn-l": "1755-1471"}, "abstract": "The proteinase-activated receptor 1 (PAR-1) plays a central role in melanoma progression and its expression level is believed to correlate with the degree of cancer invasiveness. Here, we show that PAR-1 is post-transcriptionally regulated by miR-20b microRNA in human melanoma cells. PAR-1 was found to be expressed in metastatic melanoma cells but was barely detectable in primary melanoma. By transducing primary melanoma cells with a lentivirus containing a 3'-UTR construct of PAR-1 mRNA, we could show that endogenous melanoma microRNAs interacted with PAR-1 3'-UTR and silenced a fused luciferase reporter. Transfection of an inhibitor against miR-20b into primary melanoma cells reversed this process. Finally, transfection of miR-20b mimic into metastatic melanoma cells caused downregulation of the luciferase reporter. We conclude that miR-20b regulates expression of melanoma PAR-1 receptor, which may explain the differential expression of PAR-1 observed in human melanoma.", "doi": "10.1111/pcmr.12217", "pmid": "24405508", "labels": {"Bioinformatics Support, Infrastructure and Training": null, "Bioinformatics Support and Infrastructure": null, "Bioinformatics (NBIS)": null}, "xrefs": [], "notes": [], "created": "2017-05-04T14:56:34.377Z", "modified": "2021-07-08T09:31:31.596Z"}