{"entity": "publication", "iuid": "f25799db31874a55adf3d174418bff26", "timestamp": "2026-04-12T03:17:57.291Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f25799db31874a55adf3d174418bff26.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f25799db31874a55adf3d174418bff26"}}, "title": "Transcription profiling of peripheral B cells in antibody-positive primary Sj\u00f6gren's syndrome reveals upregulated expression of CX3CR1 and a type I and type II interferon signature.", "authors": [{"family": "Imgenberg-Kreuz", "given": "J", "initials": "J", "orcid": "0000-0002-7230-8990", "researcher": {"href": "https://publications.scilifelab.se/researcher/5d4c2f630d484ee780c2c12aaabdb939.json"}}, {"family": "Sandling", "given": "J K", "initials": "JK"}, {"family": "Bj\u00f6rk", "given": "A", "initials": "A"}, {"family": "Nordlund", "given": "J", "initials": "J", "orcid": "0000-0001-8699-9959", "researcher": {"href": "https://publications.scilifelab.se/researcher/ddf48c9262134821bcc6ce1180049753.json"}}, {"family": "Kvarnstr\u00f6m", "given": "M", "initials": "M"}, {"family": "Eloranta", "given": "M-L", "initials": "ML"}, {"family": "R\u00f6nnblom", "given": "L", "initials": "L"}, {"family": "Wahren-Herlenius", "given": "M", "initials": "M", "orcid": "0000-0002-0915-7245", "researcher": {"href": "https://publications.scilifelab.se/researcher/a8451e7f5e6e4e4da0bace3dfafaeb38.json"}}, {"family": "Syv\u00e4nen", "given": "A-C", "initials": "AC", "orcid": "0000-0002-9681-9146", "researcher": {"href": "https://publications.scilifelab.se/researcher/f7012e35025543379380cb90efd71243.json"}}, {"family": "Nordmark", "given": "G", "initials": "G", "orcid": "0000-0002-3829-7431", "researcher": {"href": "https://publications.scilifelab.se/researcher/188fda53498740dbb007441cc94bb1ad.json"}}], "type": "journal article", "published": "2018-05-00", "journal": {"volume": "87", "issn": "1365-3083", "issue": "5", "pages": "e12662", "title": "Scand. J. Immunol.", "issn-l": "0300-9475"}, "abstract": "B cells play a key role in the pathogenesis of primary Sj\u00f6gren's syndrome (pSS). The aim of this study was to analyse the transcriptome of CD19+ B cells from patients with pSS and healthy controls to decipher the B cell-specific contribution to pSS. RNA from purified CD19+ B cells from 12 anti-SSA antibody-positive untreated female patients with pSS and 20 healthy blood donors was subjected to whole transcriptome sequencing. A false discovery rate corrected significance threshold of \u03b1 < 0.05 was applied to define differential gene expression. As validation, gene expression in B cells from 17 patients with pSS and 16 healthy controls was analysed using a targeted gene panel. RNA-sequencing identified 4047 differentially expressed autosomal genes in pSS B cells. Upregulated expression of type I and type II interferon (IFN)-induced genes was observed, establishing an IFN signature in pSS B cells. Among the top upregulated and validated genes were CX3CR1, encoding the fractalkine receptor involved in regulation of B-cell malignancies, CCL5/RANTES and CCR1. Increased expression of several members of the TNF superfamily was also identified; TNFSF4/Ox40L, TNFSF10/TRAIL, TNFSF13B/BAFF, TNFRSF17/BCMA as well as S100A8 and -A9/calprotectin, TLR7, STAT1 and STAT2. Among genes with downregulated expression in pSS B cells were SOCS1 and SOCS3, CD70 and TNFAIP3/A20. We conclude that B cells from patients with anti-SSA antibody-positive pSS display immune activation with upregulated expression of chemokines, chemokine receptors and a prominent type I and type II IFN signature, while suppressors of cytokine signalling are downregulated. This adds insight into the autoimmune process and suggests potential targets for future functional studies.", "doi": "10.1111/sji.12662", "pmid": "29655283", "labels": {"National Genomics Infrastructure": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [], "notes": [], "created": "2018-05-03T11:10:51.391Z", "modified": "2024-01-16T13:48:46.360Z"}