{"entity": "publication", "iuid": "ecc798da00bd489298e00c1eb42e9dad", "timestamp": "2026-08-09T05:10:19.096Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ecc798da00bd489298e00c1eb42e9dad.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ecc798da00bd489298e00c1eb42e9dad"}}, "title": "Migratory CD103+CD11b+ cDC2s in Peyer's patches are critical for gut IgA responses following oral immunization.", "authors": [{"family": "Gribonika", "given": "Inta", "initials": "I"}, {"family": "Str\u00f6mberg", "given": "Anneli", "initials": "A"}, {"family": "Chandode", "given": "Rakesh K", "initials": "RK"}, {"family": "Sch\u00f6n", "given": "Karin", "initials": "K"}, {"family": "Lahl", "given": "Katharina", "initials": "K"}, {"family": "Bemark", "given": "Mats", "initials": "M"}, {"family": "Lycke", "given": "Nils", "initials": "N"}], "type": "journal article", "published": "2024-08-00", "journal": {"title": "Mucosal Immunol", "issn": "1935-3456", "volume": "17", "issue": "4", "pages": "509-523", "issn-l": "1933-0219"}, "abstract": "Induction and regulation of specific intestinal immunoglobulin (Ig)A responses critically depend on dendritic cell (DC) subsets and the T cells they activate in the Peyer's patches (PP). We found that oral immunization with cholera toxin (CT) as an adjuvant resulted in migration-dependent changes in the composition and localization of PP DC subsets with increased numbers of cluster of differentiation (CD)103- conventional DC (cDC)2s and lysozyme-expressing DC (LysoDCs) in the subepithelial dome and of CD103+ cDC2s that expressed CD101 in the T cell zones, while oral ovalbumin (OVA) tolerization was instead associated with greater accumulation of cDC1s and peripherally induced regulatory T cells (pTregs) in this area. Decreased IgA responses were observed after CT-adjuvanted immunization in huCD207DTA mice lacking CD103+ cDC2s, while oral OVA tolerization was inefficient in cDC1-deficient Batf3-/- mice. Using OVA transgenic T cell receptor CD4 T cell adoptive transfer models, we found that co-transferred endogenous wildtype CD4 T cells can hinder the induction of OVA-specific IgA responses through secretion of interleukin-10. CT could overcome this blocking effect, apparently through a modulating effect on pTregs while promoting an expansion of follicular helper T cells. The data support a model where cDC1-induced pTreg normally suppresses PP responses for any given antigen and where CT's oral adjuvanticity effect is dependent on promoting follicular helper T cell responses through induction of CD103+ cDC2s.", "doi": "10.1016/j.mucimm.2024.03.004", "pmid": "38492746", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pii", "key": "S1933-0219(24)00023-0"}], "notes": [], "created": "2024-11-15T12:05:07.359Z", "modified": "2024-11-15T12:05:07.363Z"}