{"entity": "publication", "iuid": "e43e7e015dec4e649c76420282600e5a", "timestamp": "2026-07-13T03:20:05.268Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e43e7e015dec4e649c76420282600e5a.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e43e7e015dec4e649c76420282600e5a"}}, "title": "Identification of noncoding transcripts from within CENP-A chromatin at fission yeast centromeres.", "authors": [{"family": "Choi", "given": "Eun Shik", "initials": "ES"}, {"family": "Str\u00e5lfors", "given": "Annelie", "initials": "A"}, {"family": "Castillo", "given": "Araceli G", "initials": "AG"}, {"family": "Durand-Dubief", "given": "Micka\u00ebl", "initials": "M"}, {"family": "Ekwall", "given": "Karl", "initials": "K"}, {"family": "Allshire", "given": "Robin C", "initials": "RC"}], "type": "journal article", "published": "2011-07-01", "journal": {"volume": "286", "issn": "1083-351X", "issue": "26", "pages": "23600-23607", "title": "J. Biol. Chem.", "issn-l": "0021-9258"}, "abstract": "The histone H3 variant CENP-A is the most favored candidate for an epigenetic mark that specifies the centromere. In fission yeast, adjacent heterochromatin can direct CENP-A(Cnp1) chromatin establishment, but the underlying features governing where CENP-A(Cnp1) chromatin assembles are unknown. We show that, in addition to centromeric regions, a low level of CENP-A(Cnp1) associates with gene promoters where histone H3 is depleted by the activity of the Hrp1(Chd1) chromatin-remodeling factor. Moreover, we demonstrate that noncoding RNAs are transcribed by RNA polymerase II (RNAPII) from CENP-A(Cnp1) chromatin at centromeres. These analyses reveal a similarity between centromeres and a subset of RNAPII genes and suggest a role for remodeling at RNAPII promoters within centromeres that influences the replacement of histone H3 with CENP-A(Cnp1).", "doi": "10.1074/jbc.M111.228510", "pmid": "21531710", "labels": {"National Genomics Infrastructure": null, "NGI Stockholm (Genomics Applications)": null, "NGI Stockholm (Genomics Production)": null}, "xrefs": [{"db": "pii", "key": "M111.228510"}, {"db": "pmc", "key": "PMC3123123"}], "notes": [], "created": "2017-05-04T14:57:20.723Z", "modified": "2020-01-21T13:56:05.862Z"}