{"entity": "publication", "iuid": "e2c7e0df97dc45de9a909aafc53e6ff6", "timestamp": "2026-08-12T06:11:00.946Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e2c7e0df97dc45de9a909aafc53e6ff6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e2c7e0df97dc45de9a909aafc53e6ff6"}}, "title": "ZBED6 regulates Igf2 expression partially through its regulation of miR483 expression.", "authors": [{"family": "Naboulsi", "given": "Rakan", "initials": "R"}, {"family": "Larsson", "given": "M\u00e5rten", "initials": "M"}, {"family": "Andersson", "given": "Leif", "initials": "L", "orcid": "0000-0002-4085-6968", "researcher": {"href": "https://publications.scilifelab.se/researcher/bd3343c12f994b1fabcae23027d3a76d.json"}}, {"family": "Younis", "given": "Shady", "initials": "S"}], "type": "journal article", "published": "2021-09-30", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "11", "issue": "1", "pages": "19484", "issn-l": "2045-2322"}, "abstract": "The expression of Igf2 in mammals shows a complex regulation involving multiple promoters and epigenetic mechanisms. We previously identified a novel regulatory mechanism based on the interaction between the transcriptional factor ZBED6 and Igf2 intron. Disruption of the ZBED6-Igf2 interaction leads to a dramatic up-regulation of IGF2 expression postnatally. In the current study we characterize an additional layer of regulation involving miR483 encoded by another Igf2 intron. We found a highly significant up-regulation of miR483 expression when the ZBED6-Igf2 axis is disrupted in transgenic mice. Furthermore, CRISPR/Cas9 mediated knock-out of miR483 in C2C12 myoblast cells, both wild-type and cells with disrupted ZBED6-Igf2 axis (Igf2dGGCT), resulted in down-regulation of Igf2 expression and a reduced proliferation rate. This was further validated using miR483 mimics and inhibitors. RNA-seq analysis revealed a significant enrichment of genes involved in the PI3K-Akt signaling pathway among genes down-regulated in miR483-/- cells, including Igf2 down-regulation. The opposite pattern was observed in Igf2dGGCT cells, where Igf2 is up-regulated. Our data suggest a positive feedback between miR483 and Igf2 promoter activity, strongly affecting how ZBED6 controls Igf2 expression in various cell types.", "doi": "10.1038/s41598-021-98777-0", "pmid": "34593874", "labels": {"NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41598-021-98777-0"}, {"db": "pmc", "key": "PMC8484269"}], "notes": [], "created": "2022-01-16T20:56:35.213Z", "modified": "2022-01-16T20:56:35.229Z"}