{"entity": "publication", "iuid": "dc442da430d445dc9fb4f880fea083a9", "timestamp": "2026-09-12T19:41:22.104Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/dc442da430d445dc9fb4f880fea083a9.json"}, "display": {"href": "https://publications.scilifelab.se/publication/dc442da430d445dc9fb4f880fea083a9"}}, "title": "Parallel barcoding of antibodies for DNA-assisted proteomics.", "authors": [{"family": "Dezfouli", "given": "Mahya", "initials": "M"}, {"family": "Vickovic", "given": "Sanja", "initials": "S"}, {"family": "Iglesias", "given": "Maria Jesus", "initials": "MJ"}, {"family": "Schwenk", "given": "Jochen M", "initials": "JM", "orcid": "0000-0001-8141-8449", "researcher": {"href": "https://publications.scilifelab.se/researcher/aba5822711b246b397fffacb7ae403b3.json"}}, {"family": "Ahmadian", "given": "Afshin", "initials": "A"}], "type": "journal article", "published": "2014-11-00", "journal": {"volume": "14", "issn": "1615-9861", "issue": "21-22", "pages": "2432-2436", "title": "Proteomics", "issn-l": "1615-9853"}, "abstract": "DNA-assisted proteomics technologies enable ultra-sensitive measurements in multiplex format using DNA-barcoded affinity reagents. Although numerous antibodies are available, nowadays targeting nearly the complete human proteome, the majority is not accessible at the quantity, concentration, or purity recommended for most bio-conjugation protocols. Here, we introduce a magnetic bead-assisted DNA-barcoding approach, applicable for several antibodies in parallel, as well as reducing required reagents quantities up to a thousand-fold. The success of DNA-barcoding and retained functionality of antibodies were demonstrated in sandwich immunoassays and standard quantitative Immuno-PCR assays. Specific DNA-barcoding of antibodies for multiplex applications was presented on suspension bead arrays with read-out on a massively parallel sequencing platform in a procedure denoted Immuno-Sequencing. Conclusively, human plasma samples were analyzed to indicate the functionality of barcoded antibodies in intended proteomics applications.", "doi": "10.1002/pmic.201400215", "pmid": "25263329", "labels": {"National Genomics Infrastructure": null, "NGI Stockholm (Genomics Applications)": null, "NGI Stockholm (Genomics Production)": null, "Affinity Proteomics Stockholm": "Technology development"}, "xrefs": [], "notes": [], "created": "2017-05-04T14:58:59.241Z", "modified": "2021-07-08T12:07:34.293Z"}