{"entity": "publication", "iuid": "d8b81b81144a4b4c87d482492b60c637", "timestamp": "2026-10-27T19:08:01.346Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d8b81b81144a4b4c87d482492b60c637.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d8b81b81144a4b4c87d482492b60c637"}}, "title": "MicroRNA profiles of prostate cancer bone metastases and miR-375 as an inhibitor of the aggressive metastasis subtype MetB.", "authors": [{"family": "J\u00e4remo", "given": "Helena", "initials": "H"}, {"family": "Semenas", "given": "Julius", "initials": "J"}, {"family": "Halin Bergstr\u00f6m", "given": "Sofia", "initials": "S"}, {"family": "Thysell", "given": "Elin", "initials": "E"}, {"family": "Lundholm", "given": "Marie", "initials": "M"}, {"family": "Freyhult", "given": "Eva", "initials": "E"}, {"family": "Josefsson", "given": "Andreas", "initials": "A"}, {"family": "Wel\u00e9n", "given": "Karin", "initials": "K"}, {"family": "Thellenberg Karlsson", "given": "Camilla", "initials": "C"}, {"family": "Crnalic", "given": "Sead", "initials": "S"}, {"family": "Bergh", "given": "Anders", "initials": "A"}, {"family": "Brattsand", "given": "Maria", "initials": "M"}, {"family": "Wikstr\u00f6m", "given": "Pernilla", "initials": "P", "orcid": "0000-0002-6347-1999", "researcher": {"href": "https://publications.scilifelab.se/researcher/66e1e640e83948a4a3f8a1ddd49bd953.json"}}], "type": "journal article", "published": "2026-08-25", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "16", "issue": "1", "issn-l": "2045-2322"}, "abstract": "Novel treatments for metastatic prostate cancer (PC) are needed. Molecular subtypes of bone metastatic PC (MetA-C) were recently identified, with the proliferation-driven MetB subtype linked to very poor prognosis. Non-coding microRNAs (miRs) regulate gene expression and constitute potential therapeutic targets. This study aimed to explore miR profiles in bone metastases in relation to the MetA-C subtypes, with focus on the aggressive MetB subtype. Genome-wide microarray analysis of clinical bone metastasis samples (n = 96, 62 patients) identified miR-375 to be inversely associated with the MetB subtype. The metastatic C4-2B cell line was transduced to overexpress or sequester miR-375 and effects were evaluated by microarray analysis, cell proliferation/adhesion assays, and tumor growth in mice. miR-375 overexpression in C4-2B altered transcription profiles and shifted the phenotype from being MetB-like to becoming MetA-like. In cell culture, miR-375 overexpression reduced cell proliferation, increased adhesion, and downregulated YAP1 expression among other direct gene targets, while miR-375 inhibition had opposite effects. In mice, miR-375 overexpression reduced tumor establishment and growth. Our data link clinical and experimental findings of miR-375 being a potential inhibitor of the aggressive MetB subtype in PC.", "doi": "10.1038/s41598-026-67032-9", "pmid": "42642447", "labels": {"Bioinformatics Support, Infrastructure and Training": "Collaborative", "Bioinformatics Support and Infrastructure": "Collaborative", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC13507049"}, {"db": "pii", "key": "10.1038/s41598-026-67032-9"}], "notes": [], "created": "2026-10-26T15:41:07.482Z", "modified": "2026-10-26T15:41:07.496Z"}