{"entity": "publication", "iuid": "ce4e0d1017bd4a9a92ef181ba3ca099a", "timestamp": "2026-07-19T19:28:26.888Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ce4e0d1017bd4a9a92ef181ba3ca099a.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ce4e0d1017bd4a9a92ef181ba3ca099a"}}, "title": "USP18 Impacts Cisplatin Resistance in Ovarian Cancer Cells by Modulating DNA Repair.", "authors": [{"family": "Corno", "given": "Cristina", "initials": "C"}, {"family": "Costantino", "given": "Matteo", "initials": "M"}, {"family": "Pettinari", "given": "Pietro", "initials": "P"}, {"family": "Mirra", "given": "Luca", "initials": "L"}, {"family": "Stucchi", "given": "Simone", "initials": "S"}, {"family": "Arrighetti", "given": "Noemi", "initials": "N"}, {"family": "Perta", "given": "Nunzio", "initials": "N"}, {"family": "Di Muccio", "given": "Giovanni", "initials": "G"}, {"family": "Robin", "given": "Mathilde", "initials": "M"}, {"family": "Beretta", "given": "Giovanni L", "initials": "GL"}, {"family": "Corna", "given": "Elisabetta", "initials": "E"}, {"family": "Carenini", "given": "Nives", "initials": "N"}, {"family": "Cleris", "given": "Loredana", "initials": "L"}, {"family": "Colombo", "given": "Diego", "initials": "D"}, {"family": "Luison", "given": "Elena", "initials": "E"}, {"family": "Ciniselli", "given": "Chiara Maura", "initials": "CM"}, {"family": "Lecchi", "given": "Mara", "initials": "M"}, {"family": "Verderio", "given": "Paolo", "initials": "P"}, {"family": "Figini", "given": "Mariangela", "initials": "M"}, {"family": "Linder", "given": "Stig", "initials": "S"}, {"family": "Tosi", "given": "Diego", "initials": "D"}, {"family": "La Teana", "given": "Anna", "initials": "A"}, {"family": "D'Arcy", "given": "Padraig", "initials": "P"}, {"family": "Perego", "given": "Paola", "initials": "P"}], "type": "journal article", "published": "2026-05-29", "journal": {"title": "Int. J. Biol. Sci.", "issn": "1449-2288", "volume": "22", "issue": "11", "pages": "5780-5798", "issn-l": "1449-2288"}, "abstract": "Deubiquitinases (DUBs) are proteases with emerging roles in cancer, yet their contribution to drug resistance in ovarian cancer remains underexplored. Ovarian cancer patients often fail to benefit from platinum-based therapy, highlighting the need to identify novel factors driving drug resistance. Thus, we performed a CRISPR/Cas9 screen targeting the DUB family to identify genes essential for cisplatin-resistant ovarian carcinoma cell survival. CRISPR/Cas9 DUB knockout screens, preclinical pharmacology approaches, RNA sequencing, proteomic analyses, computational tools, surface plasma resonance were applied. We identified USP18 as a survival factor in cisplatin-resistant ovarian cancer cells. USP18 expression was elevated at the mRNA and protein levels across five cisplatin-resistant variants. Knockdown and CRISPR/Cas9 editing of USP18 sensitized cells to cisplatin, coinciding with impaired repair of cisplatin-induced DNA damage. Enhanced sensitivity to cisplatin was evident from studies in mice. RNA-seq of USP18 RNA interfered and edited cells revealed the modulation of pathways including DNA repair. A peptide-based USP18 inhibitor suppressed growth of cisplatin-resistant cells, supporting USP18 role in sustaining their growth. We identified USP18 as a novel mediator of cisplatin resistance in ovarian cancer, acting through DNA repair modulation. Targeting USP18 may offer a therapeutic strategy to improve outcomes in platinum-resistant ovarian cancer.", "doi": "10.7150/ijbs.130399", "pmid": "42328450", "labels": {"CRISPR Functional Genomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC13282739"}, {"db": "pii", "key": "ijbsv22p5780"}], "notes": [], "created": "2026-07-13T09:09:05.184Z", "modified": "2026-07-13T09:09:05.204Z"}