{"entity": "publication", "iuid": "c6ac13283afd4ea48ddc2b39be64df94", "timestamp": "2026-04-11T07:27:40.629Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c6ac13283afd4ea48ddc2b39be64df94.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c6ac13283afd4ea48ddc2b39be64df94"}}, "title": "Cellular Barcoding Links B-1a B Cell Potential to a Fetal Hematopoietic Stem Cell State at the Single-Cell Level.", "authors": [{"family": "Kristiansen", "given": "Trine A", "initials": "TA"}, {"family": "Jaensson Gyllenb\u00e4ck", "given": "Elin", "initials": "E"}, {"family": "Zriwil", "given": "Alya", "initials": "A"}, {"family": "Bj\u00f6rklund", "given": "Tomas", "initials": "T"}, {"family": "Daniel", "given": "Jeremy A", "initials": "JA"}, {"family": "Sitnicka", "given": "Ewa", "initials": "E"}, {"family": "Soneji", "given": "Shamit", "initials": "S"}, {"family": "Bryder", "given": "David", "initials": "D"}, {"family": "Yuan", "given": "Joan", "initials": "J"}], "type": "journal article", "published": "2016-08-16", "journal": {"volume": "45", "issn": "1097-4180", "issue": "2", "pages": "346-357", "title": "Immunity", "issn-l": "1074-7613"}, "abstract": "Hematopoietic stem cells (HSCs) undergo a functional switch in neonatal mice hallmarked by a decrease in self-renewing divisions and entry into quiescence. Here, we investigated whether the developmental attenuation of B-1a cell output is a consequence of a shift in stem cell state during ontogeny. Using cellular barcoding for in\u00a0vivo single-cell fate analyses, we found that fetal liver definitive HSCs gave rise to both B-1a and B-2 cells. Whereas B-1a potential diminished in all HSCs with time, B-2 output was maintained. B-1a and B-2 plasticity could be reinitiated in a subset of adult HSCs by ectopic expression of the RNA binding protein LIN28B, a key regulator of fetal hematopoiesis, and this coincided with the clonal reversal to fetal-like elevated self-renewal and repopulation potential. These results anchor the attenuation of B-1a cell output to fetal HSC behavior and demonstrate that the developmental decline in regenerative potential represents a reversible HSC state.", "doi": "10.1016/j.immuni.2016.07.014", "pmid": "27533015", "labels": {"National Genomics Infrastructure": "Service", "NGI Uppsala (Uppsala Genome Center)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pii", "key": "S1074-7613(16)30287-4"}], "notes": [], "created": "2017-05-03T12:59:52.312Z", "modified": "2024-01-16T13:48:49.647Z"}