{"entity": "publication", "iuid": "c28dc85e503f4ad8b3fbe19acd8f4bcf", "timestamp": "2026-07-11T04:55:30.727Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c28dc85e503f4ad8b3fbe19acd8f4bcf.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c28dc85e503f4ad8b3fbe19acd8f4bcf"}}, "title": "Darkened Leaves Use Different Metabolic Strategies for Senescence and Survival.", "authors": [{"family": "Law", "given": "Simon R", "initials": "SR"}, {"family": "Chrobok", "given": "Daria", "initials": "D", "orcid": "0000-0002-4842-7690", "researcher": {"href": "https://publications.scilifelab.se/researcher/a4db812aeb344bc1b1bd61a149ed163b.json"}}, {"family": "Juvany", "given": "Marta", "initials": "M", "orcid": "0000-0003-1093-3317", "researcher": {"href": "https://publications.scilifelab.se/researcher/e1c5bd691038499bbc1a4265b22b192a.json"}}, {"family": "Delhomme", "given": "Nicolas", "initials": "N", "orcid": "0000-0002-3053-0796", "researcher": {"href": "https://publications.scilifelab.se/researcher/107fbbd40f1444fb838ad4c0365738fa.json"}}, {"family": "Lind\u00e9n", "given": "Pernilla", "initials": "P"}, {"family": "Brouwer", "given": "Bastiaan", "initials": "B", "orcid": "0000-0002-6609-982X", "researcher": {"href": "https://publications.scilifelab.se/researcher/fe8be89e1d614381aac8a1311fad1ff6.json"}}, {"family": "Ahad", "given": "Abdul", "initials": "A"}, {"family": "Moritz", "given": "Thomas", "initials": "T", "orcid": "0000-0002-4258-3190", "researcher": {"href": "https://publications.scilifelab.se/researcher/95ad5b7fe48f42eda1328f54a385e097.json"}}, {"family": "Jansson", "given": "Stefan", "initials": "S", "orcid": "0000-0002-7906-6891", "researcher": {"href": "https://publications.scilifelab.se/researcher/fb9d3c17f4514903b3731d15c622a53d.json"}}, {"family": "Gardestr\u00f6m", "given": "Per", "initials": "P", "orcid": "0000-0001-5900-7395", "researcher": {"href": "https://publications.scilifelab.se/researcher/767df182c20a466c95227f285419407f.json"}}, {"family": "Keech", "given": "Olivier", "initials": "O", "orcid": "0000-0002-0546-7721", "researcher": {"href": "https://publications.scilifelab.se/researcher/cbfa829eb0b74b67aed7865dda0e15d3.json"}}], "type": "journal article", "published": "2018-05-00", "journal": {"title": "Plant Physiol.", "issn": "1532-2548", "volume": "177", "issue": "1", "pages": "132-150", "issn-l": "0032-0889"}, "abstract": "In plants, an individually darkened leaf initiates senescence much more rapidly than a leaf from a whole darkened plant. Combining transcriptomic and metabolomic approaches in Arabidopsis ( Arabidopsis thaliana), we present an overview of the metabolic strategies that are employed in response to different darkening treatments. Under darkened plant conditions, the perception of carbon starvation drove a profound metabolic readjustment in which branched-chain amino acids and potentially monosaccharides released from cell wall loosening became important substrates for maintaining minimal ATP production. Concomitantly, the increased accumulation of amino acids with a high nitrogen-carbon ratio may provide a safety mechanism for the storage of metabolically derived cytotoxic ammonium and a pool of nitrogen for use upon returning to typical growth conditions. Conversely, in individually darkened leaf, the metabolic profiling that followed our 13C-enrichment assays revealed a temporal and differential exchange of metabolites, including sugars and amino acids, between the darkened leaf and the rest of the plant. This active transport could be the basis for a progressive metabolic shift in the substrates fueling mitochondrial activities, which are central to the catabolic reactions facilitating the retrieval of nutrients from the senescing leaf. We propose a model illustrating the specific metabolic strategies employed by leaves in response to these two darkening treatments, which support either rapid senescence or a strong capacity for survival.", "doi": "10.1104/pp.18.00062", "pmid": "29523713", "labels": {"Swedish Metabolomics Centre": "Service"}, "xrefs": [{"db": "pii", "key": "pp.18.00062"}, {"db": "pmc", "key": "PMC5933110"}], "notes": [], "created": "2019-01-10T10:01:37.175Z", "modified": "2025-10-17T13:03:18.171Z"}