{"entity": "publication", "iuid": "c1ee33b53e234983b5dc0bb7365058df", "timestamp": "2026-07-10T08:39:46.212Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/c1ee33b53e234983b5dc0bb7365058df.json"}, "display": {"href": "https://publications.scilifelab.se/publication/c1ee33b53e234983b5dc0bb7365058df"}}, "title": "Selected \u03b2-glucans act as immune-training agents by improving anti-mycobacterial activity in human macrophages - a pilot study.", "authors": [{"family": "Braian", "given": "Clara", "initials": "C"}, {"family": "Karlsson", "given": "Lovisa", "initials": "L"}, {"family": "Das", "given": "Jyotirmoy", "initials": "J"}, {"family": "Lerm", "given": "Maria", "initials": "M"}], "type": "news", "published": "2023-09-21", "journal": {"title": "J Innate Immun", "issn": "1662-8128", "volume": "15", "issue": "1", "pages": "751-764", "issn-l": "1662-811X"}, "abstract": "Epigenetic reprogramming of innate immune cells by \u03b2-glucan in a process called trained immunity, leads to an enhanced host response to a secondary infection. \u03b2-glucans are structural components of plants, algae, fungi and bacteria and thus recognized as non-self by human macrophages. We selected the \u03b2-glucans curdlan from Alcaligenes faecalis, WGP dispersible from Saccharomyces cerevisiae, and \u03b2-glucan-rich culture supernatant of Alternaria and investigated whether they could produce trained immunity effects leading to an increased control of virulent Mycobacterium tuberculosis. We observed a significant M. tuberculosis growth-reduction in macrophages trained with curdlan and Alternaria, which also correlated with increased IL-6 and IL-1\u03b2 release. WGP dispersible-trained macrophages were stratified into 'non responders' and 'responders', according to their ability to control M. tuberculosis, with 'responders' producing higher IL-6 levels. The addition of neutrophils to infected macrophage cultures further enhanced macrophage control of virulent M. tuberculosis, but not in a stimuli-dependent manner. Pathway enrichment analysis of DNA methylome data also highlighted hypomethylation of genes in pathways associated with signaling and cellular reorganization and motility, and 'responders' to WGP-training were enriched in the interferon-gamma signaling pathway. This study adds evidence that certain \u03b2-glucans show promise as immune training agents.", "doi": "10.1159/000533873", "pmid": "37734337", "labels": {"Clinical Genomics Link\u00f6ping": "Collaborative", "Clinical Genomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC10616672"}, {"db": "pii", "key": "000533873"}], "notes": [], "created": "2023-12-03T06:08:06.478Z", "modified": "2023-12-03T06:08:06.481Z"}