{"entity": "publication", "iuid": "a37a5acc6c434e50afcfc8a6a13a8700", "timestamp": "2026-07-11T06:13:42.153Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a37a5acc6c434e50afcfc8a6a13a8700.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a37a5acc6c434e50afcfc8a6a13a8700"}}, "title": "Structural basis of specific lysine transport by Pseudomonas aeruginosa permease LysP.", "authors": [{"family": "Bicer", "given": "Deniz", "initials": "D"}, {"family": "Matsuoka", "given": "Rei", "initials": "R", "orcid": "0000-0001-6718-2572", "researcher": {"href": "https://publications.scilifelab.se/researcher/2ff469bc61824dfdbd2f1bd2d9eda8fb.json"}}, {"family": "Moumbock", "given": "Aur\u00e9lien F A", "initials": "AFA", "orcid": "0000-0002-6034-2016", "researcher": {"href": "https://publications.scilifelab.se/researcher/d82e18423c15489da2e66f44bc546208.json"}}, {"family": "Sukumar", "given": "Preethi", "initials": "P"}, {"family": "Suades", "given": "Albert", "initials": "A"}, {"family": "Cheruvara", "given": "Harish", "initials": "H"}, {"family": "Quigley", "given": "Andrew", "initials": "A", "orcid": "0000-0002-5022-9845", "researcher": {"href": "https://publications.scilifelab.se/researcher/3463df3e436f4accbef2c85d631d535b.json"}}, {"family": "Drew", "given": "David", "initials": "D"}, {"family": "Pardon", "given": "Els", "initials": "E", "orcid": "0000-0002-2466-0172", "researcher": {"href": "https://publications.scilifelab.se/researcher/375719a54c844150a4d5cb2b4f3ebb57.json"}}, {"family": "Steyaert", "given": "Jan", "initials": "J", "orcid": "0000-0002-3825-874X", "researcher": {"href": "https://publications.scilifelab.se/researcher/2a13eb5511e1448fb982b5bd1bb1d6f1.json"}}, {"family": "Henderson", "given": "Peter J F", "initials": "PJF", "orcid": "0000-0002-9187-0938", "researcher": {"href": "https://publications.scilifelab.se/researcher/9a039ab6f9b946079084fa5c5a1d73cb.json"}}, {"family": "Caffrey", "given": "Martin", "initials": "M", "orcid": "0000-0002-2931-4551", "researcher": {"href": "https://publications.scilifelab.se/researcher/4860054d06e24ccfb39fe781305f73d8.json"}}, {"family": "Griese", "given": "Julia J", "initials": "JJ", "orcid": "0000-0003-3686-3062", "researcher": {"href": "https://publications.scilifelab.se/researcher/308d2df438d8443ca932fb5111e4590e.json"}}, {"family": "Nji", "given": "Emmanuel", "initials": "E", "orcid": "0000-0001-6991-1046", "researcher": {"href": "https://publications.scilifelab.se/researcher/88dfdecdfcb44a97b8513508e294aebd.json"}}], "type": "journal article", "published": "2025-12-04", "journal": {"title": "Nat Commun", "issn": "2041-1723", "issn-l": "2041-1723"}, "abstract": "Under conditions of extreme acidity, the lysine-specific permease, LysP, not only mediates the import of L-lysine it also interacts with the transcriptional regulator, CadC, to activate expression of the cadAB operon. This operon encodes the lysine decarboxylase, CadA, which converts lysine to cadaverine while consuming a cytoplasmic proton, and the antiporter, CadB, which exports protonated cadaverine in exchange for extracellular lysine. Together, these processes contribute to cytoplasmic pH homeostasis and support bacterial acid resistance - a mechanism essential for the survival of pathogenic bacteria in acidic host environments. Here, we present the cryo-EM structure of LysP from Pseudomonas aeruginosa in an inward-occluded conformation (3.2-5.3 \u00c5 resolution), bound to L-lysine and a nanobody. L-Lysine is coordinated by hydrophobic contacts, cation-\u03c0 interactions, and by hydrogen bonding mostly with polar uncharged residues. Reconstitution of LysP into proteoliposomes confirms specific L-lysine transport, which is competitively inhibited by L-4-thialysine. These findings provide a structural framework for understanding selective lysine recognition and inhibition, with implications for antibacterial drug design.", "doi": "10.1038/s41467-025-66618-7", "pmid": "41345107", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41467-025-66618-7"}], "notes": [], "created": "2025-12-09T13:04:51.180Z", "modified": "2025-12-09T13:04:57.345Z"}