{"entity": "publication", "iuid": "a0c7031690744d69bd94427f6d63b73c", "timestamp": "2026-08-08T03:40:08.012Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a0c7031690744d69bd94427f6d63b73c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a0c7031690744d69bd94427f6d63b73c"}}, "title": "Genome-wide estrogen receptor \u03b2 chromatin binding in human colon cancer cells reveals its tumor suppressor activity.", "authors": [{"family": "Indukuri", "given": "Rajitha", "initials": "R", "orcid": "0000-0001-6570-842X", "researcher": {"href": "https://publications.scilifelab.se/researcher/94148ec0ffb74f9bb0243c18a1256c22.json"}}, {"family": "Jafferali", "given": "Mohammed Hakim", "initials": "MH"}, {"family": "Song", "given": "Dandan", "initials": "D"}, {"family": "Damdimopoulos", "given": "Anastasios", "initials": "A"}, {"family": "Hases", "given": "Linnea", "initials": "L"}, {"family": "Zhao", "given": "Chunyan", "initials": "C"}, {"family": "Archer", "given": "Amena", "initials": "A", "orcid": "0000-0002-0400-4151", "researcher": {"href": "https://publications.scilifelab.se/researcher/4502538fe3e84cb6a6618c972fa10b08.json"}}, {"family": "Williams", "given": "Cecilia", "initials": "C", "orcid": "0000-0002-0602-2062", "researcher": {"href": "https://publications.scilifelab.se/researcher/89989da8d4e64dd3a30b87fc62ceefae.json"}}], "type": "journal article", "published": "2021-08-01", "journal": {"title": "Int. J. Cancer", "issn": "1097-0215", "volume": "149", "issue": "3", "pages": "692-706", "issn-l": "0020-7136"}, "abstract": "Colorectal cancer (CRC) is the third leading cause of cancer death in the western world. In women, menopausal hormone therapy has been shown to reduce CRC incidence by 20%. Studies demonstrate that estrogen activating estrogen receptor beta (ER\u03b2) protects against CRC. ER\u03b2 is a nuclear receptor that regulates gene expression through interactions with the chromatin. This molecular mechanism is, however, not well characterized in colon. Here, we present for the first time, the cistrome of ER\u03b2 in different colon cancer cell lines. We use cell lines engineered to express ER\u03b2, optimize and validate an ER\u03b2 antibody for chromatin-immunoprecipitation (ChIP), and perform ChIP-Seq. We identify key binding motifs, including ERE, AP-1, and TCF sites, and we determine enrichment of binding to cis-regulatory chromatin sites of genes involved in tumor development, cell migration, cell adhesion, apoptosis, and Wnt signaling pathways. We compare the corresponding cistromes of colon and breast cancer and find that they are conserved for about a third of genes, including GREB1, but that ER\u03b2 tethering to TCF and KLF family motifs is characteristic for colon. We exemplify upregulation of putative CRC tumor suppressor gene CST5 where ER\u03b2 in colon cells binds to cis-regulatory regions nearby (-351 bp) the transcriptional start site. Our work provides a foundation for understanding the mechanism of action of ER\u03b2 in CRC prevention.", "doi": "10.1002/ijc.33573", "pmid": "33754337", "labels": {"NGI Stockholm (Genomics Production)": null, "NGI Stockholm (Genomics Applications)": null, "National Genomics Infrastructure": null, "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [], "notes": [], "created": "2021-06-09T12:16:18.247Z", "modified": "2024-01-16T13:48:38.912Z"}