{"entity": "publication", "iuid": "a0c0acf600de4866b5133701a7e87eb5", "timestamp": "2026-08-10T22:44:37.947Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/a0c0acf600de4866b5133701a7e87eb5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/a0c0acf600de4866b5133701a7e87eb5"}}, "title": "TMAO enhances TNF-\u03b1 mediated fibrosis and release of inflammatory mediators from renal fibroblasts.", "authors": [{"family": "Stefania", "given": "Kapetanaki", "initials": "K"}, {"family": "Ashok", "given": "Kumawat Kumar", "initials": "KK"}, {"family": "Geena", "given": "Paramel Varghese", "initials": "PV"}, {"family": "Katarina", "given": "Persson", "initials": "P"}, {"family": "Isak", "given": "Demirel", "initials": "D"}], "type": "journal article", "published": "2024-04-20", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "14", "issue": "1", "pages": "9070", "issn-l": "2045-2322"}, "abstract": "Trimethylamine-N-oxide (TMAO) is a gut microbiota-derived metabolite and TNF-\u03b1 is proinflammatory cytokine, both known to be associated with renal inflammation, fibrosis and chronic kidney disease. However, today there are no data showing the combined effect of TMAO and TNF-\u03b1 on renal fibrosis-and inflammation. The aim of this study was to investigate whether TMAO can enhance the inflammatory and fibrotic effects of TNF-\u03b1 on renal fibroblasts. We found that the combination of TNF-\u03b1 and TMAO synergistically increased fibronectin release and total collagen production from renal fibroblasts. The combination of TMAO and TNF-\u03b1 also promoted increased cell proliferation. Both renal proliferation and collagen production were mediated through Akt/mTOR/ERK signaling. We also found that TMAO enhanced TNF-\u03b1 mediated renal inflammation by inducing the release of several cytokines (IL-6, LAP TGF-beta-1), chemokines (CXCL-6, MCP-3), inflammatory-and growth mediators (VEGFA, CD40, HGF) from renal fibroblasts. In conclusion, we showed that TMAO can enhance TNF-\u03b1 mediated renal fibrosis and release of inflammatory mediators from renal fibroblasts in vitro. Our results can promote further research evaluating the combined effect of TMAO and inflammatory mediators on the development of kidney disease.", "doi": "10.1038/s41598-024-58084-w", "pmid": "38643262", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11032383"}, {"db": "pii", "key": "10.1038/s41598-024-58084-w"}], "notes": [], "created": "2024-11-27T22:23:39.656Z", "modified": "2024-11-27T22:23:39.661Z"}