{"entity": "publication", "iuid": "9d51310e57cd402e986a3d86d8e8e9b2", "timestamp": "2026-10-17T09:59:35.729Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/9d51310e57cd402e986a3d86d8e8e9b2.json"}, "display": {"href": "https://publications.scilifelab.se/publication/9d51310e57cd402e986a3d86d8e8e9b2"}}, "title": "Spatial Transcriptomic Assessments of Gene Expression Profiles in Human Peri-Implant Lesions.", "authors": [{"family": "Dionigi", "given": "C", "initials": "C", "orcid": "0000-0002-4195-0729", "researcher": {"href": "https://publications.scilifelab.se/researcher/5ee57750bddd48a3a323e397c64ba435.json"}}, {"family": "Ericson", "given": "A C", "initials": "AC"}, {"family": "Shikhan", "given": "A", "initials": "A"}, {"family": "Larsson", "given": "L", "initials": "L"}, {"family": "Berglundh", "given": "T", "initials": "T"}], "type": "journal article", "published": "2026-01-00", "journal": {"title": "J Clin Periodontol", "issn": "1600-051X", "volume": "53", "issue": "1", "pages": "137-151", "issn-l": null}, "abstract": "To analyse gene expression profiles in human tissue samples obtained from dental implant sites with or without peri-implantitis.\n\nSoft-tissue biopsy samples obtained from patients presenting with implants with severe peri-implantitis and adjacent reference implants with no signs of peri-implantitis were analysed by spatial transcriptomics (n = 4), RNA-sequencing (n = 20) and immunohistochemistry (n = 38).\n\nThe analysis disclosed evident associations between distinct gene clusters and specific tissue compartments in both groups of specimens. Samples from peri-implantitis sites showed higher levels of gene activity than samples from reference implant sites. Gene-set enrichment analysis revealed that several important biological pathways, for example, 'regulation of antimicrobial host response', 'collagen fibril organisation' and 'regulation of angiogenesis', were severely dysregulated in peri-implantitis lesions when compared to reference implant sites. Similarly, immunohistochemical evaluation showed elevated levels of proteins corresponding to the most up-regulated, differentially expressed genes (DEGs), which were identified by spatial transcriptomics and RNA-sequencing.\n\nPeri-implant lesions demonstrated associations between gene clusters and specific tissue compartments. Several biological pathways linked to the activation of host response towards bacterial insults were dysregulated in peri-implantitis specimens when compared to reference implant samples.", "doi": "10.1111/jcpe.70008", "pmid": "41077421", "labels": {"NGI Spatial omics": "Service", "NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Applications)": "Service", "Clinical Genomics Gothenburg": "Service", "Clinical Genomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12695443"}], "notes": [], "created": "2026-10-14T13:05:44.902Z", "modified": "2026-10-16T15:07:34.303Z"}