{"entity": "publication", "iuid": "8d4a8412b91143359d590e01636497ae", "timestamp": "2026-09-08T18:24:40.343Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/8d4a8412b91143359d590e01636497ae.json"}, "display": {"href": "https://publications.scilifelab.se/publication/8d4a8412b91143359d590e01636497ae"}}, "title": "Extracellular vesicle surface engineering with integrins (ITGAL & ITGB2) to specifically target ICAM-1-expressing endothelial cells.", "authors": [{"family": "Bergqvist", "given": "Markus", "initials": "M", "orcid": "0009-0003-5716-3716", "researcher": {"href": "https://publications.scilifelab.se/researcher/91d33e374d0642479a80683a989f095a.json"}}, {"family": "Park", "given": "Kyong-Su", "initials": "KS", "orcid": "0000-0003-0902-7800", "researcher": {"href": "https://publications.scilifelab.se/researcher/26e308b29fe144b49f4db27f4b819c18.json"}}, {"family": "Karimi", "given": "Nasibeh", "initials": "N", "orcid": "0000-0003-1499-6876", "researcher": {"href": "https://publications.scilifelab.se/researcher/14632eec875c42038aa0b6983e0ec107.json"}}, {"family": "Yu", "given": "Lijuan", "initials": "L", "orcid": "0000-0003-3558-3800", "researcher": {"href": "https://publications.scilifelab.se/researcher/8b0b4ca8553144b19c236bc09c1c9b81.json"}}, {"family": "L\u00e4sser", "given": "Cecilia", "initials": "C", "orcid": "0000-0003-1279-1746", "researcher": {"href": "https://publications.scilifelab.se/researcher/e14e17d2cdb24a9f93991d83811c78b9.json"}}, {"family": "L\u00f6tvall", "given": "Jan", "initials": "J", "orcid": "0000-0001-9195-9249", "researcher": {"href": "https://publications.scilifelab.se/researcher/0cbcaf6b5698411c92e0de9e8fcf390f.json"}}], "type": "journal article", "published": "2025-01-30", "journal": {"title": "J Nanobiotechnology", "issn": "1477-3155", "volume": "23", "issue": "1", "pages": "64", "issn-l": null}, "abstract": "Extracellular vesicles (EVs) are taken up by most cells, however specific or preferential cell targeting remains a hurdle. This study aims to develop an EV that targets cells involved in inflammation, specifically those expressing intercellular adhesion molecule-1 (ICAM-1). To target these cells, we overexpress the ICAM-1 binding receptor \"lymphocyte function-associated antigen-1\" (LFA-1) in HEK293F cells, by sequential transfection of plasmids of the two LFA-1 subunits, ITGAL and ITGB2 (CD11a and CD18). The LFA-1 receptor was strongly overexpressed on the EVs released by the transfected cells. We further loaded these EVs with a therapeutic peptide, targeting myeloid differentiation primary response 88 (Myd88; EVMyd88), through a developed EV open-and-close procedure. Myd88 is a downstream common intracellular messenger for most TLR-receptors. EV expression of LFA-1 increases EV binding to ICAM-1-expressing cells, an effect that was dose-dependently inhibited by a specific neutralizing ICAM-1 antibody. Further, activated human endothelial cells treated with LFA-1 EVMyd88 had increased uptake of these EVs, resulting in dose-dependent inhibition of induced release of IL-8, presumably by targeting Myd88. We conclude that LFA-1-expressing EVMyd88 may be a candidate suitable for delivering therapeutic peptides in inflammatory diseases associated with TLR-activation.", "doi": "10.1186/s12951-025-03125-3", "pmid": "39885580", "labels": {"Glycoproteomics and MS Proteomics": "Service", "Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11780982"}, {"db": "pii", "key": "10.1186/s12951-025-03125-3"}], "notes": [], "created": "2025-10-23T13:06:45.347Z", "modified": "2025-11-05T13:48:02.553Z"}