{"entity": "publication", "iuid": "7f45bd70f2f443a5a2f012f81cd0eaab", "timestamp": "2026-10-19T15:54:14.418Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/7f45bd70f2f443a5a2f012f81cd0eaab.json"}, "display": {"href": "https://publications.scilifelab.se/publication/7f45bd70f2f443a5a2f012f81cd0eaab"}}, "title": "The spindle-associated antisense RNA ARHGEF17-AS1 functionally contributes to mitotic spindle integrity.", "authors": [{"family": "Mahale", "given": "Sagar", "initials": "S"}, {"family": "Setia", "given": "Meenakshi", "initials": "M"}, {"family": "Sj\u00f6vall", "given": "Daniel", "initials": "D"}, {"family": "Deshpande", "given": "Ananya", "initials": "A"}, {"family": "Nagarajan", "given": "Kamali", "initials": "K"}, {"family": "Reischl", "given": "Silke", "initials": "S"}, {"family": "Jaako", "given": "Pekka", "initials": "P"}, {"family": "Kanduri", "given": "Meena", "initials": "M"}, {"family": "Kanduri", "given": "Chandrasekhar", "initials": "C"}], "type": "journal article", "published": "2026-09-22", "journal": {"title": "Cell Rep", "issn": "2211-1247", "volume": "45", "issue": "10", "pages": "118023", "issn-l": null}, "abstract": "Long non-coding RNAs (lncRNAs) are increasingly recognized as regulators of mitotic progression, yet few have been shown to act directly at the mitotic spindle. Here we identify ARHGEF17-AS1 as a metaphase-enriched antisense RNA that localizes to the mitotic spindle and is associated with spindle pole integrity. ARHGEF17-AS1 interacts with RPS3 and the dynein motor complex and is associated with RPS3 recruitment to the mitotic spindle and with the RPS3-dynein interaction. Depletion of ARHGEF17-AS1 reduces this interaction and decreases RPS3 accumulation at spindle poles. ARHGEF17-AS1 depletion is also associated with lower RPS3 abundance, reduced RNF10 expression, and loss of mitosis-associated RPS3 monoubiquitination at lysine 214. These perturbations coincide with altered spindle microtubule organization and delayed mitotic progression. Together, the data support a model in which ARHGEF17-AS1 contributes to spindle organization through an RNA-mediated pathway involving extra-ribosomal RPS3, dynein, and RNF10, supporting an important role for ARHGEF17-AS1 in mitotic spindle organization.", "doi": "10.1016/j.celrep.2026.118023", "pmid": "42771527", "labels": {"Clinical Genomics Gothenburg": "Service", "Clinical Genomics": "Service"}, "xrefs": [{"db": "pii", "key": "S2211-1247(26)01101-0"}], "notes": [], "created": "2026-10-16T15:07:36.278Z", "modified": "2026-10-16T15:07:36.306Z"}