{"entity": "publication", "iuid": "7dc1eec982f6407b9279d515c64d5013", "timestamp": "2026-07-22T23:35:39.203Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/7dc1eec982f6407b9279d515c64d5013.json"}, "display": {"href": "https://publications.scilifelab.se/publication/7dc1eec982f6407b9279d515c64d5013"}}, "title": "Transcriptomic profiling of the oocyte-cumulus-granulosa cell complex from estrogen receptor \u03b2 knockout mice.", "authors": [{"family": "T\u00f6h\u00f6nen", "given": "Virpi", "initials": "V"}, {"family": "Antonson", "given": "Per", "initials": "P"}, {"family": "Boggavarapu", "given": "Nageswara Rao", "initials": "NR"}, {"family": "Ali", "given": "Heba", "initials": "H"}, {"family": "Motaholi", "given": "Leticia Apolinario", "initials": "LA"}, {"family": "Gustafsson", "given": "Jan-\u00c5ke", "initials": "J\u00c5"}, {"family": "Varshney", "given": "Mukesh", "initials": "M"}, {"family": "Rodriguez-Wallberg", "given": "Kenny A", "initials": "KA"}, {"family": "Katayama", "given": "Shintaro", "initials": "S"}, {"family": "Nalvarte", "given": "Ivan", "initials": "I"}, {"family": "Inzunza", "given": "Jose", "initials": "J"}], "type": "journal article", "published": "2024-11-00", "journal": {"title": "F S Sci", "issn": "2666-335X", "volume": "5", "issue": "4", "pages": "306-317", "issn-l": null}, "abstract": "To study the role of estrogen receptor \u03b2 in follicle development and maturation and the response to gonadotropin stimulation aiming at superovulation.\n\nExperimental study and transcriptomic analysis.\n\nKarolinka Institutet, medical university.\n\nHealthy wild-type (WT) and estrogen receptor \u03b2 knockout (Esr2-KO) female mice undergoing superovulation at 4 weeks, 7 weeks, and 6 months of age.\n\nNot applicable.\n\nOocyte yield after superovulation, transcriptomic profiling of cumulus-granulosa cell complexes and oocytes, and immunohistochemical analyses.\n\nSuperovulation of estrogen receptor \u03b2 (ER\u03b2) knockout mice resulted in reduced oocyte yield at 6 months of age compared with WT mice, but younger mice had similar yields. RNA-seq analysis of cumulus cells from superovulated WT and Esr2-KO mice identified genes and pathways associated with among others adhesion, proliferation, Wnt-signaling, and placed ER\u03b2 in bipotential granulosa cell cluster. Loss of ER\u03b2 increased expression of the other estrogen receptors Esr1 and Gper1.\n\nOur results show that ER\u03b2 has an important role in regulating ovulation in response to exogenous gonadotropins in 6-month-old mice, but not in younger mice. Our transcriptomic and immunohistochemical observations suggest a dysregulation of the granulosa cell communication and lack of tight coordination between granulosa cell replication and antrum expansion. A significant upregulation of other estrogen receptors may support a compensatory mechanism sustaining fertility during younger age in Esr2-KO mice.", "doi": "10.1016/j.xfss.2024.08.004", "pmid": "39168303", "labels": {"NGI Short read": "Service", "National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Production)": "Service"}, "xrefs": [{"db": "pii", "key": "S2666-335X(24)00056-9"}], "notes": [], "created": "2025-09-03T12:06:32.682Z", "modified": "2025-09-03T12:06:32.731Z"}