{"entity": "publication", "iuid": "7aff62d808ef404a8b5a402908f43327", "timestamp": "2026-07-22T14:41:06.346Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/7aff62d808ef404a8b5a402908f43327.json"}, "display": {"href": "https://publications.scilifelab.se/publication/7aff62d808ef404a8b5a402908f43327"}}, "title": "Dysregulation in Akt/mTOR/HIF-1 signaling identified by proteo-transcriptomics of SARS-CoV-2 infected cells.", "authors": [{"family": "Appelberg", "given": "Sofia", "initials": "S"}, {"family": "Gupta", "given": "Soham", "initials": "S"}, {"family": "Svensson Akusj\u00e4rvi", "given": "Sara", "initials": "S"}, {"family": "Ambikan", "given": "Anoop T", "initials": "AT"}, {"family": "Mikaeloff", "given": "Flora", "initials": "F"}, {"family": "Saccon", "given": "Elisa", "initials": "E"}, {"family": "V\u00e9gv\u00e1ri", "given": "\u00c1kos", "initials": "\u00c1"}, {"family": "Benfeitas", "given": "Rui", "initials": "R"}, {"family": "Sperk", "given": "Maike", "initials": "M"}, {"family": "St\u00e5hlberg", "given": "Marie", "initials": "M"}, {"family": "Krishnan", "given": "Shuba", "initials": "S"}, {"family": "Singh", "given": "Kamal", "initials": "K"}, {"family": "Penninger", "given": "Josef M", "initials": "JM"}, {"family": "Mirazimi", "given": "Ali", "initials": "A"}, {"family": "Neogi", "given": "Ujjwal", "initials": "U", "orcid": "0000-0002-0844-3338", "researcher": {"href": "https://publications.scilifelab.se/researcher/2f8094017c2a4d0a94d72813cab526f7.json"}}], "type": "journal article", "published": "2020-12-00", "journal": {"title": "Emerg Microbes Infect", "issn": "2222-1751", "volume": "9", "issue": "1", "pages": "1748-1760", "issn-l": "2222-1751"}, "abstract": "How severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infections engage cellular host pathways and innate immunity in infected cells remains largely elusive. We performed an integrative proteo-transcriptomics analysis in SARS-CoV-2 infected Huh7 cells to map the cellular response to the invading virus over time. We identified four pathways, ErbB, HIF-1, mTOR and TNF signaling, among others that were markedly modulated during the course of the SARS-CoV-2 infection in vitro. Western blot validation of the downstream effector molecules of these pathways revealed a dose-dependent activation of Akt, mTOR, S6K1 and 4E-BP1 at 24 hours post infection (hpi). However, we found a significant inhibition of HIF-1\u03b1 through 24hpi and 48hpi of the infection, suggesting a crosstalk between the SARS-CoV-2 and the Akt/mTOR/HIF-1 signaling pathways. Inhibition of the mTOR signaling pathway using Akt inhibitor MK-2206 showed a significant reduction in virus production. Further investigations are required to better understand the molecular sequelae in order to guide potential therapy in the management of severe coronavirus disease 2019 (COVID-19) patients.", "doi": "10.1080/22221751.2020.1799723", "pmid": "32691695", "labels": {"Bioinformatics Support and Infrastructure": "Service", "Bioinformatics Support, Infrastructure and Training": "Service", "Bioinformatics Support for Computational Resources": "Service", "Bioinformatics (NBIS)": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC7473213"}], "notes": [], "created": "2020-09-24T09:28:47.205Z", "modified": "2024-01-16T13:48:41.261Z"}