{"entity": "publication", "iuid": "71de611ecb654cf29bce7e2b76610cbe", "timestamp": "2026-10-19T22:58:36.446Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/71de611ecb654cf29bce7e2b76610cbe.json"}, "display": {"href": "https://publications.scilifelab.se/publication/71de611ecb654cf29bce7e2b76610cbe"}}, "title": "Dissecting placental host-pathogen interactions: Rift Valley fever virus infection in early human trophoblast stem cells.", "authors": [{"family": "Gwon", "given": "Yong-Dae", "initials": "YD"}, {"family": "Haider", "given": "Sandra", "initials": "S"}, {"family": "Kn\u00f6fler", "given": "Martin", "initials": "M"}, {"family": "Bradley", "given": "Matthew", "initials": "M"}, {"family": "Henriksson", "given": "Johan", "initials": "J"}, {"family": "Evander", "given": "Magnus", "initials": "M"}], "type": "journal article", "published": "2026-05-15", "journal": {"title": "iScience", "issn": "2589-0042", "volume": "29", "issue": "5", "pages": "115584", "issn-l": "2589-0042"}, "abstract": "Rift Valley fever virus (RVFV) is a mosquito-borne Phlebovirus and zoonotic pathogen affecting maternal-fetal health. Vertical transmission is linked to miscarriage and severe fetal outcomes, but mechanisms of placental pathogenesis remain unclear. We used first-trimester human trophoblast stem cells (hTSCs) to model infection at the maternal-fetal interface. Immunofluorescence, qRT-PCR, western blotting, and single-cell transcriptomics showed that hTSCs are highly susceptible to RVFV. Strand-specific viral transcriptomics confirmed the ambisense S segment and revealed preferential transcription of the M and S segments over L. RVFV induced G1 arrest, impairing trophoblast proliferation and differentiation, and drove widespread transcriptional reprogramming, including strong interferon lambda 1 (IFNL1) but modest type I interferon responses, and dysregulation of inflammatory and preeclampsia-associated genes such as RUNX1 and TGFBRAP1. Recombinant IFN-\u03bb pretreatment reduced RVFV protein expression, highlighting hTSCs as a robust model and IFN-\u03bb as a promising antiviral strategy.", "doi": "10.1016/j.isci.2026.115584", "pmid": "42023155", "labels": {"NGI Stockholm (Genomics Production)": "Service", "National Genomics Infrastructure": "Service", "NGI Short read": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC13098504"}, {"db": "pii", "key": "S2589-0042(26)00959-4"}], "notes": [], "created": "2026-10-14T12:34:18.658Z", "modified": "2026-10-14T12:34:18.666Z"}