{"entity": "publication", "iuid": "4cd28ffb0bb040aeb1d18f01821866e5", "timestamp": "2026-08-10T23:34:31.666Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/4cd28ffb0bb040aeb1d18f01821866e5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/4cd28ffb0bb040aeb1d18f01821866e5"}}, "title": "Expression of chondroitin/dermatan sulfate glycosyltransferases during early zebrafish development.", "authors": [{"family": "Filipek-G\u00f3rniok", "given": "Beata", "initials": "B", "orcid": "0000-0002-6757-5410", "researcher": {"href": "https://publications.scilifelab.se/researcher/11f0b7b3e0b045f082d2aff1dd23ec0e.json"}}, {"family": "Holmborn", "given": "Katarina", "initials": "K"}, {"family": "Haitina", "given": "Tatjana", "initials": "T"}, {"family": "Habicher", "given": "Judith", "initials": "J"}, {"family": "Oliveira", "given": "Marta Bastos", "initials": "MB"}, {"family": "Hellgren", "given": "Charlotte", "initials": "C"}, {"family": "Eriksson", "given": "Inger", "initials": "I"}, {"family": "Kjell\u00e9n", "given": "Lena", "initials": "L"}, {"family": "Kreuger", "given": "Johan", "initials": "J"}, {"family": "Ledin", "given": "Johan", "initials": "J", "orcid": "0000-0002-7319-7735", "researcher": {"href": "https://publications.scilifelab.se/researcher/92e482abc18c49d881d3bf0132b3fbcd.json"}}], "type": "journal article", "published": "2013-08-00", "journal": {"volume": "242", "issn": "1097-0177", "issue": "8", "pages": "964-975", "title": "Dev. Dyn.", "issn-l": "1058-8388"}, "abstract": "Chondroitin/dermatan sulfate (CS/DS) proteoglycans present in the extracellular matrix have important structural and regulatory functions.\n\nSix human genes have previously been shown to catalyze CS/DS polymerization. Here we show that one of these genes, chpf, is represented by two copies in the zebrafish genome, chpfa and chpfb, while the other five human CS/DS glycosyltransferases csgalnact1, csgalnact2, chpf2, chsy1, and chsy3 all have single zebrafish orthologues. The putative zebrafish CS/DS glycosyltransferases are spatially and temporally expressed. Interestingly, overlapping expression of multiple glycosyltransferases coincides with high CS/DS deposition. Finally, whereas the relative levels of the related polysaccharide HS reach steady-state at around 2 days post fertilization, there is a continued relative increase of the CS amounts per larvae during the first 6 days of development, matching the increased cartilage formation.\n\nThere are 7 CS/DS glycosyltransferases in zebrafish, which, based on homology, can be divided into the CSGALNACT, CHSY, and CHPF families. The overlap between intense CS/DS production and the expression of multiple CS/DS glycosyltransferases suggests that efficient CS/DS biosynthesis requires a combination of several glycosyltransferases.", "doi": "10.1002/dvdy.23981", "pmid": "23703795", "labels": {"Genome Engineering Zebrafish": null}, "xrefs": [], "notes": [], "created": "2017-05-04T15:03:43.737Z", "modified": "2021-07-06T16:05:51.247Z"}