{"entity": "publication", "iuid": "4b0eeb6b9dbe4f5bb00e055228ef7146", "timestamp": "2026-09-06T20:50:15.127Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/4b0eeb6b9dbe4f5bb00e055228ef7146.json"}, "display": {"href": "https://publications.scilifelab.se/publication/4b0eeb6b9dbe4f5bb00e055228ef7146"}}, "title": "Adaptations in metabolism and protein translation give rise to the Crabtree effect in yeast.", "authors": [{"family": "Malina", "given": "Carl", "initials": "C", "orcid": "0000-0001-7855-5311", "researcher": {"href": "https://publications.scilifelab.se/researcher/d6c98aaa2a8841d995e7544da08d6825.json"}}, {"family": "Yu", "given": "Rosemary", "initials": "R", "orcid": "0000-0001-9901-4055", "researcher": {"href": "https://publications.scilifelab.se/researcher/d0ef0147940a4ad2920f703175a748ed.json"}}, {"family": "Bj\u00f6rkeroth", "given": "Johan", "initials": "J", "orcid": "0000-0002-2263-4886", "researcher": {"href": "https://publications.scilifelab.se/researcher/cffa10d5ff634326b3bb7e0835772e56.json"}}, {"family": "Kerkhoven", "given": "Eduard J", "initials": "EJ", "orcid": "0000-0002-3593-5792", "researcher": {"href": "https://publications.scilifelab.se/researcher/0df361f8014144e79479631fcbffad53.json"}}, {"family": "Nielsen", "given": "Jens", "initials": "J", "orcid": "0000-0002-9955-6003", "researcher": {"href": "https://publications.scilifelab.se/researcher/7a596e289be4438a8a2653b1f25fea8b.json"}}], "type": "journal article", "published": "2021-12-21", "journal": {"title": "Proc. Natl. Acad. Sci. U.S.A.", "issn": "1091-6490", "volume": "118", "issue": "51", "issn-l": "0027-8424"}, "abstract": "Aerobic fermentation, also referred to as the Crabtree effect in yeast, is a well-studied phenomenon that allows many eukaryal cells to attain higher growth rates at high glucose availability. Not all yeasts exhibit the Crabtree effect, and it is not known why Crabtree-negative yeasts can grow at rates comparable to Crabtree-positive yeasts. Here, we quantitatively compared two Crabtree-positive yeasts, Saccharomyces cerevisiae and Schizosaccharomyces pombe, and two Crabtree-negative yeasts, Kluyveromyces marxianus and Scheffersomyces stipitis, cultivated under glucose excess conditions. Combining physiological and proteome quantification with genome-scale metabolic modeling, we found that the two groups differ in energy metabolism and translation efficiency. In Crabtree-positive yeasts, the central carbon metabolism flux and proteome allocation favor a glucose utilization strategy minimizing proteome cost as proteins translation parameters, including ribosomal content and/or efficiency, are lower. Crabtree-negative yeasts, however, use a strategy of maximizing ATP yield, accompanied by higher protein translation parameters. Our analyses provide insight into the underlying reasons for the Crabtree effect, demonstrating a coupling to adaptations in both metabolism and protein translation.", "doi": "10.1073/pnas.2112836118", "pmid": "34903663", "labels": {"Glycoproteomics and MS Proteomics": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC8713813"}, {"db": "pii", "key": "2112836118"}], "notes": [], "created": "2023-03-07T14:35:22.349Z", "modified": "2024-01-16T13:46:29.769Z"}