{"entity": "publication", "iuid": "4a6ebd48c6af40f79918a31bb57a0488", "timestamp": "2026-08-10T23:28:31.919Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/4a6ebd48c6af40f79918a31bb57a0488.json"}, "display": {"href": "https://publications.scilifelab.se/publication/4a6ebd48c6af40f79918a31bb57a0488"}}, "title": "The role of caspase-1, caspase-4 and NLRP3 in regulating the host cell response evoked by uropathogenic Escherichia coli.", "authors": [{"family": "Lindblad", "given": "Anna", "initials": "A"}, {"family": "Johansson", "given": "Charlotte", "initials": "C"}, {"family": "Persson", "given": "Katarina", "initials": "K"}, {"family": "Demirel", "given": "Isak", "initials": "I"}], "type": "journal article", "published": "2022-02-07", "journal": {"title": "Sci Rep", "issn": "2045-2322", "volume": "12", "issue": "1", "pages": "2005", "issn-l": "2045-2322"}, "abstract": "The inflammasome-associated proteins caspase-1, caspase-4 and NLRP3 have been emphasised to be essential in the host cell response during urinary tract infection (UTI) by regulating IL-1\u03b2 release. Our aim was to investigate how the inflammasome-associated proteins regulate the cell response of bladder epithelial cells during infection with uropathogenic Escherichia coli (UPEC). Human bladder epithelial cells (5637) and CRISPR/Cas9 generated caspase-1, caspase-4 and NLRP3 knockdown cells were stimulated with the UPEC strain CFT073. Using Olink proteomics and real time RT-PCR, we showed that caspase-1, caspase-4 and NLRP3 are vital for the expression of many inflammatory genes and proteins from bladder epithelial cells. When investigating the effect of inflammasome-associated proteins on neutrophils, we found that conditioned medium from UPEC-infected caspase-4 knockdown cells significantly increased phagocytosis of CFT073 and significantly decreased ROS production from neutrophils. In contrast, conditioned medium from UPEC-infected NLRP3 knockdown cells significantly decreased the phagocytosis of CFT073 and significantly increased the ROS production from neutrophils. In conclusion, we showed that the inflammasome-associated proteins contribute to the host cell response during UPEC infection.", "doi": "10.1038/s41598-022-06052-7", "pmid": "35132157", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC8821701"}, {"db": "pii", "key": "10.1038/s41598-022-06052-7"}], "notes": [], "created": "2023-11-29T19:13:01.325Z", "modified": "2023-11-29T19:13:01.338Z"}