{"entity": "publication", "iuid": "4981f4e297634c109a11f7a33ef378df", "timestamp": "2026-05-19T06:30:28.216Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/4981f4e297634c109a11f7a33ef378df.json"}, "display": {"href": "https://publications.scilifelab.se/publication/4981f4e297634c109a11f7a33ef378df"}}, "title": "Muscle wasting and the temporal gene expression pattern in a novel rat intensive care unit model.", "authors": [{"family": "Llano-Diez", "given": "Monica", "initials": "M"}, {"family": "Gustafson", "given": "Ann-Marie", "initials": "AM"}, {"family": "Olsson", "given": "Carl", "initials": "C"}, {"family": "Goransson", "given": "Hanna", "initials": "H"}, {"family": "Larsson", "given": "Lars", "initials": "L"}], "type": "journal article", "published": "2011-12-13", "journal": {"volume": "12", "issn": "1471-2164", "issue": null, "pages": "602", "title": "BMC Genomics", "issn-l": "1471-2164"}, "abstract": "Acute quadriplegic myopathy (AQM) or critical illness myopathy (CIM) is frequently observed in intensive care unit (ICU) patients. To elucidate duration-dependent effects of the ICU intervention on molecular and functional networks that control the muscle wasting and weakness associated with AQM, a gene expression profile was analyzed at time points varying from 6 hours to 14 days in a unique experimental rat model mimicking ICU conditions, i.e., post-synaptically paralyzed, mechanically ventilated and extensively monitored animals.\n\nDuring the observation period, 1583 genes were significantly up- or down-regulated by factors of two or greater. A significant temporal gene expression pattern was constructed at short (6 h-4 days), intermediate (5-8 days) and long (9-14 days) durations. A striking early and maintained up-regulation (6 h-14d) of muscle atrogenes (muscle ring-finger 1/tripartite motif-containing 63 and F-box protein 32/atrogin-1) was observed, followed by an up-regulation of the proteolytic systems at intermediate and long durations (5-14d). Oxidative stress response genes and genes that take part in amino acid catabolism, cell cycle arrest, apoptosis, muscle development, and protein synthesis together with myogenic factors were significantly up-regulated from 5 to 14 days. At 9-14 d, genes involved in immune response and the caspase cascade were up-regulated. At 5-14d, genes related to contractile (myosin heavy chain and myosin binding protein C), regulatory (troponin, tropomyosin), developmental, caveolin-3, extracellular matrix, glycolysis/gluconeogenesis, cytoskeleton/sarcomere regulation and mitochondrial proteins were down-regulated. An activation of genes related to muscle growth and new muscle fiber formation (increase of myogenic factors and JunB and down-regulation of myostatin) and up-regulation of genes that code protein synthesis and translation factors were found from 5 to 14 days.\n\nNovel temporal patterns of gene expression have been uncovered, suggesting a unique, coordinated and highly complex mechanism underlying the muscle wasting associated with AQM in ICU patients and providing new target genes and avenues for intervention studies.", "doi": "10.1186/1471-2164-12-602", "pmid": "22165895", "labels": {"Array and Analysis Facility": null}, "xrefs": [{"db": "pii", "key": "1471-2164-12-602"}, {"db": "pmc", "key": "PMC3266306"}], "notes": [], "created": "2017-05-04T15:02:51.187Z", "modified": "2017-05-30T14:49:24.252Z"}