{"entity": "publication", "iuid": "463f9c74eb7f4ac3b30f4d31e9faa2ab", "timestamp": "2026-09-14T03:34:15.131Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/463f9c74eb7f4ac3b30f4d31e9faa2ab.json"}, "display": {"href": "https://publications.scilifelab.se/publication/463f9c74eb7f4ac3b30f4d31e9faa2ab"}}, "title": "A Dual-Halogen Labeling Strategy to Detect and Quantify Double-Stranded RNAs Using NanoSIMS: A Proof-of-Concept Study.", "authors": [{"family": "Vicentini", "given": "Quentin", "initials": "Q", "orcid": "0009-0006-7659-6164", "researcher": {"href": "https://publications.scilifelab.se/researcher/e828ca0fda334d5d891720c2328bdc79.json"}}, {"family": "Kurczy", "given": "Michael", "initials": "M", "orcid": "0000-0001-6579-9691", "researcher": {"href": "https://publications.scilifelab.se/researcher/02749b50f73548da89f25ee2a0fd30c1.json"}}, {"family": "Estupi\u00f1\u00e1n", "given": "H Yesid", "initials": "HY"}, {"family": "Hekman", "given": "Dennis", "initials": "D"}, {"family": "Becquart", "given": "C\u00e9cile", "initials": "C"}, {"family": "Haag", "given": "Lars", "initials": "L"}, {"family": "Andersson", "given": "Shalini", "initials": "S"}, {"family": "El-Andaloussi", "given": "Samir", "initials": "S"}, {"family": "Dahl\u00e9n", "given": "Anders", "initials": "A", "orcid": "0000-0002-2641-7918", "researcher": {"href": "https://publications.scilifelab.se/researcher/230d4758846244bf881fa6d6e06257cf.json"}}], "type": "journal article", "published": "2026-08-25", "journal": {"title": "ACS Omega", "issn": "2470-1343", "issn-l": "2470-1343", "volume": "11", "issue": "33", "pages": "49739-49745"}, "abstract": "Oligonucleotide drugs, such as small interfering RNAs (siRNAs) and small activating RNAs (saRNAs), are rapidly advancing in the clinic. However, a complete picture of their intracellular uptake, trafficking, and fate remains elusive. Additionally, the double-stranded nature of the compounds makes it challenging to track both the guide and passenger strands individually. To address this, we present a proof-of-concept study of nanoscale secondary ion mass spectrometry (NanoSIMS) in combination with a dual-labeling strategy using 5-bromo- and 5-iodo-modified uracil to detect, discriminate, and quantify both strands without relying on bulky fluorescent tags. By evaluating GalNAc-conjugated siRNAs and saRNAs in HepG2 cells, we demonstrate that NanoSIMS can effectively codetect guide and passenger strands within the endolysosomal compartments. For the siRNA design, imaging revealed, as expected, a robust correlation between the strands. Conversely, we highlight the use of NanoSIMS as a potential aid in the optimization of guide strand stability by exploring different chemical designs for the saRNA. This work highlights the potential of NanoSIMS to quantitatively monitor individual double-stranded RNA strands and their subcellular localization in vitro, offering a valuable tool to generate critical trafficking and quantitative data for oligonucleotide therapeutics.", "doi": "10.1021/acsomega.6c04146", "pmid": "42662369", "labels": {"NanoSIMS": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC13520541"}], "notes": [], "created": "2026-09-13T21:59:45.510Z", "modified": "2026-09-13T22:05:22.276Z"}