{"entity": "publication", "iuid": "40f9f3fc1b444b0e93af1e0487695f12", "timestamp": "2026-07-20T15:53:56.331Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/40f9f3fc1b444b0e93af1e0487695f12.json"}, "display": {"href": "https://publications.scilifelab.se/publication/40f9f3fc1b444b0e93af1e0487695f12"}}, "title": "PDE2A2 regulates mitochondria morphology and apoptotic cell death via local modulation of cAMP/PKA signalling.", "authors": [{"family": "Monterisi", "given": "Stefania", "initials": "S", "orcid": "0000-0002-8802-9742", "researcher": {"href": "https://publications.scilifelab.se/researcher/3b139738d17449fc9a91465d3e5ad9cc.json"}}, {"family": "Lobo", "given": "Miguel J", "initials": "MJ"}, {"family": "Livie", "given": "Craig", "initials": "C"}, {"family": "Castle", "given": "John C", "initials": "JC", "orcid": "0000-0002-6017-7794", "researcher": {"href": "https://publications.scilifelab.se/researcher/8da2f3d0e8e448b1ad6b2c090db980c6.json"}}, {"family": "Weinberger", "given": "Michael", "initials": "M"}, {"family": "Baillie", "given": "George", "initials": "G"}, {"family": "Surdo", "given": "Nicoletta C", "initials": "NC"}, {"family": "Musheshe", "given": "Nshunge", "initials": "N"}, {"family": "Stangherlin", "given": "Alessandra", "initials": "A", "orcid": "0000-0001-7296-1183", "researcher": {"href": "https://publications.scilifelab.se/researcher/e6b0df4758a140ccad8bf23cd15f85ea.json"}}, {"family": "Gottlieb", "given": "Eyal", "initials": "E"}, {"family": "Maizels", "given": "Rory", "initials": "R"}, {"family": "Bortolozzi", "given": "Mario", "initials": "M", "orcid": "0000-0001-7198-9838", "researcher": {"href": "https://publications.scilifelab.se/researcher/35b1c39a3d1d43608e14d7eba3107ca2.json"}}, {"family": "Micaroni", "given": "Massimo", "initials": "M"}, {"family": "Zaccolo", "given": "Manuela", "initials": "M", "orcid": "0000-0002-0934-3662", "researcher": {"href": "https://publications.scilifelab.se/researcher/959c5abbe3ec4af4adb37d5549ba2d5c.json"}}], "type": "journal article", "published": "2017-05-02", "journal": {"title": "Elife", "issn": "2050-084X", "volume": "6", "issue": null, "pages": null, "issn-l": "2050-084X"}, "abstract": "cAMP/PKA signalling is compartmentalised with tight spatial and temporal control of signal propagation underpinning specificity of response. The cAMP-degrading enzymes, phosphodiesterases (PDEs), localise to specific subcellular domains within which they control local cAMP levels and are key regulators of signal compartmentalisation. Several components of the cAMP/PKA cascade are located to different mitochondrial sub-compartments, suggesting the presence of multiple cAMP/PKA signalling domains within the organelle. The function and regulation of these domains remain largely unknown. Here, we describe a novel cAMP/PKA signalling domain localised at mitochondrial membranes and regulated by PDE2A2. Using pharmacological and genetic approaches combined with real-time FRET imaging and high resolution microscopy, we demonstrate that in rat cardiac myocytes and other cell types mitochondrial PDE2A2 regulates local cAMP levels and PKA-dependent phosphorylation of Drp1. We further demonstrate that inhibition of PDE2A, by enhancing the hormone-dependent cAMP response locally, affects mitochondria dynamics and protects from apoptotic cell death.", "doi": "10.7554/eLife.21374", "pmid": "28463107", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC5423767"}], "notes": [], "created": "2020-01-23T16:35:40.005Z", "modified": "2021-06-21T15:38:15.280Z"}