{"entity": "publication", "iuid": "366b9dcbbd5948908195760c17d29905", "timestamp": "2026-07-22T14:21:15.251Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/366b9dcbbd5948908195760c17d29905.json"}, "display": {"href": "https://publications.scilifelab.se/publication/366b9dcbbd5948908195760c17d29905"}}, "title": "Doublet decoding of tRNASer3 demonstrates plasticity of ribosomal decoding center.", "authors": [{"family": "Krishnaswamy", "given": "Shruthi", "initials": "S", "orcid": "0009-0004-3486-4939", "researcher": {"href": "https://publications.scilifelab.se/researcher/e3419361bb4d468997ff52ecd38dfc51.json"}}, {"family": "Akbar", "given": "Shirin", "initials": "S", "orcid": "0000-0003-2437-9679", "researcher": {"href": "https://publications.scilifelab.se/researcher/f4fff2c547b64e3c81e0c935f911028a.json"}}, {"family": "Larsson", "given": "Daniel S D", "initials": "DSD", "orcid": "0000-0001-7683-6419", "researcher": {"href": "https://publications.scilifelab.se/researcher/9e2b9321c1064208b9c0a1b11dde571d.json"}}, {"family": "Chen", "given": "Yang", "initials": "Y"}, {"family": "Selmer", "given": "Maria", "initials": "M", "orcid": "0000-0001-9079-2774", "researcher": {"href": "https://publications.scilifelab.se/researcher/860221451df4451b8c035ff4cf25c812.json"}}], "type": "journal article", "published": "2025-06-26", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "16", "issue": "1", "pages": "5402", "issn-l": "2041-1723"}, "abstract": "Frameshifts can be caused by specific combinations of tRNA and mRNA. The wildtype AGC-decoding E. coli tRNASer3GCU has been shown to induce -1 ribosomal frameshifting on GCA alanine codons, and proposed to read a two-base codon instead of a canonical triplet. However, it has remained unclear whether this type of non-cognate decoding can be accommodated by the ribosome. Here, we perform single-particle cryo-EM reconstructions on E. coli 70S ribosomes with the frameshift-inducing tRNASer3 bound to the non-cognate GCA codon or the cognate AGC codon in the ribosomal A site. The structures demonstrate that doublet decoding is made possible when A1493, the conserved monitoring base in 16S rRNA, mimics a first codon base, forming a Hoogsteen base pair with U36 from the anticodon and stacking with the mRNA. This interaction pushes the first two bases of the A-site codon in position for base pairing with C35 and G34 of the anticodon.", "doi": "10.1038/s41467-025-61016-5", "pmid": "40571681", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12202706"}, {"db": "pii", "key": "10.1038/s41467-025-61016-5"}], "notes": [], "created": "2025-10-29T14:57:32.646Z", "modified": "2025-10-29T14:57:53.989Z"}