{"entity": "publication", "iuid": "331439f75b96458c8eb2c119329d0ac9", "timestamp": "2026-09-12T20:26:51.194Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/331439f75b96458c8eb2c119329d0ac9.json"}, "display": {"href": "https://publications.scilifelab.se/publication/331439f75b96458c8eb2c119329d0ac9"}}, "title": "MicroRNA-155 mediates multiple gene regulations pertinent to the role of human adipose-derived mesenchymal stem cells in skin regeneration.", "authors": [{"family": "Shahin", "given": "Hady", "initials": "H"}, {"family": "Belcastro", "given": "Luigi", "initials": "L"}, {"family": "Das", "given": "Jyotirmoy", "initials": "J"}, {"family": "Perdiki Grigoriadi", "given": "Marina", "initials": "M"}, {"family": "Saager", "given": "Rolf B", "initials": "RB"}, {"family": "Steinvall", "given": "Ingrid", "initials": "I"}, {"family": "Sj\u00f6berg", "given": "Folke", "initials": "F"}, {"family": "Olofsson", "given": "Pia", "initials": "P"}, {"family": "Elmasry", "given": "Moustafa", "initials": "M"}, {"family": "El-Serafi", "given": "Ahmed T", "initials": "AT"}], "type": "journal article", "published": "2024-03-18", "journal": {"title": "Front Bioeng Biotechnol", "issn": "2296-4185", "volume": "12", "pages": "1328504", "issn-l": null}, "abstract": "Introduction: The role of Adipose-derived mesenchymal stem cells (AD-MSCs) in skin wound healing remains to be fully characterized. This study aims to evaluate the regenerative potential of autologous AD-MSCs in a non-healing porcine wound model, in addition to elucidate key miRNA-mediated epigenetic regulations that underlie the regenerative potential of AD-MSCs in wounds. Methods: The regenerative potential of autologous AD-MSCs was evaluated in porcine model using histopathology and spatial frequency domain imaging. Then, the correlations between miRNAs and proteins of AD-MSCs were evaluated using an integration analysis in primary human AD-MSCs in comparison to primary human keratinocytes. Transfection study of AD-MSCs was conducted to validate the bioinformatics data. Results: Autologous porcine AD-MSCs improved wound epithelialization and skin properties in comparison to control wounds. We identified 26 proteins upregulated in human AD-MSCs, including growth and angiogenic factors, chemokines and inflammatory cytokines. Pathway enrichment analysis highlighted cell signalling-associated pathways and immunomodulatory pathways. miRNA-target modelling revealed regulations related to genes encoding for 16 upregulated proteins. miR-155-5p was predicted to regulate Fibroblast growth factor 2 and 7, C-C motif chemokine ligand 2 and Vascular cell adhesion molecule 1. Transfecting human AD-MSCs cell line with anti-miR-155 showed transient gene silencing of the four proteins at 24 h post-transfection. Discussion: This study proposes a positive miR-155-mediated gene regulation of key factors involved in wound healing. The study represents a promising approach for miRNA-based and cell-free regenerative treatment for difficult-to-heal wounds. The therapeutic potential of miR-155 and its identified targets should be further explored in-vivo.", "doi": "10.3389/fbioe.2024.1328504", "pmid": "38562669", "labels": {"Clinical Genomics Link\u00f6ping": "Collaborative", "Clinical Genomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC10982420"}, {"db": "pii", "key": "1328504"}], "notes": [], "created": "2024-12-10T08:36:50.057Z", "modified": "2024-12-10T08:36:58.034Z"}