{"entity": "publication", "iuid": "2951d91afe5d4b5b9392f91279290395", "timestamp": "2026-09-12T04:03:47.493Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2951d91afe5d4b5b9392f91279290395.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2951d91afe5d4b5b9392f91279290395"}}, "title": "Structural characterization of ribosome recycling and fusidic acid inhibition in Staphylococcus aureus.", "authors": [{"family": "Gonz\u00e1lez-L\u00f3pez", "given": "Adri\u00e1n", "initials": "A", "orcid": "0000-0002-4302-6855", "researcher": {"href": "https://publications.scilifelab.se/researcher/4cdef9bebbf940a5812ce94fa81519e9.json"}}, {"family": "Selmer", "given": "Maria", "initials": "M", "orcid": "0000-0001-9079-2774", "researcher": {"href": "https://publications.scilifelab.se/researcher/860221451df4451b8c035ff4cf25c812.json"}}], "type": "journal article", "published": "2026-08-10", "journal": {"title": "Nucleic Acids Res.", "issn": "1362-4962", "volume": "54", "issue": "15", "issn-l": "0305-1048"}, "abstract": "During bacterial ribosome recycling, 70S ribosomes are split into subunits by ribosome recycling factor (RRF) and elongation factor G (EF-G). The antibiotic fusidic acid (FA) inhibits elongation and ribosome recycling by locking EF-G to the ribosome. Yet, no functional ribosome recycling FA complex has been successfully captured. Here, we used single-particle cryo-electron microscopy to resolve multiple FA-stalled intermediates of Staphylococcus aureus ribosomes, including a 70S intermediate with RRF and EF-G in a previously unobserved conformation. Our structures reveal how RRF and EF-G jointly disrupt inter-subunit bridges, promote back-rotation of the small subunit, and move the transfer RNA toward the E site to facilitate ribosome splitting. We further show that FA predominantly inhibits recycling by trapping EF-G on the post-termination complex in the absence of RRF, preventing formation of the active RRF\u2022EF-G complex. These insights advance understanding of the molecular mechanism of bacterial ribosome recycling and the mode of action of FA as an antibiotic.", "doi": "10.1093/nar/gkag778", "pmid": "42573073", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC13454839"}, {"db": "pii", "key": "8757947"}], "notes": [], "created": "2026-09-11T11:46:00.178Z", "modified": "2026-09-11T11:46:01.726Z"}