{"entity": "publication", "iuid": "2542fca589754fc69c85633b9604f02c", "timestamp": "2026-07-15T23:19:01.107Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2542fca589754fc69c85633b9604f02c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2542fca589754fc69c85633b9604f02c"}}, "title": "Melanoma cells produce multiple laminin isoforms and strongly migrate on \u03b15 laminin(s) via several integrin receptors.", "authors": [{"family": "Oikawa", "given": "Yuko", "initials": "Y"}, {"family": "Hansson", "given": "Johan", "initials": "J"}, {"family": "Sasaki", "given": "Takako", "initials": "T"}, {"family": "Rousselle", "given": "Patricia", "initials": "P"}, {"family": "Domogatskaya", "given": "Anna", "initials": "A"}, {"family": "Rodin", "given": "Sergey", "initials": "S"}, {"family": "Tryggvason", "given": "Karl", "initials": "K"}, {"family": "Patarroyo", "given": "Manuel", "initials": "M"}], "type": "journal article", "published": "2011-05-01", "journal": {"volume": "317", "issn": "1090-2422", "issue": "8", "pages": "1119-1133", "title": "Exp. Cell Res.", "issn-l": "0014-4827"}, "abstract": "Melanoma cells express and interact with laminins (LMs) and other basement membrane components during invasion and metastasis. In the present study we have investigated the production and migration-promoting activity of laminin isoforms in melanoma. Immunohistochemistry of melanoma specimens and immunoprecipitation/western blotting of melanoma cell lines indicated expression of laminin-111/121, laminin-211, laminin-411/421, and laminin-511/521. Laminin-332 was not detected. In functional assays, laminin-111, laminin-332, and laminin-511, but not laminin-211 and laminin-411, strongly promoted haptotactic cell migration either constitutively or following stimulation with insulin-like growth factors. Both placenta and recombinant laminin-511 preparations were highly active, and the isolated recombinant IVa domain of LM\u03b15 also promoted cell migration. Function-blocking antibodies in cell migration assays revealed \u03b16\u03b21 integrin as the major receptor for laminin-111, and both \u03b13\u03b21 and \u03b16\u03b21 integrins for laminin-332 and laminin-511. In contrast, isolated LM\u03b15 IVa domain-promoted melanoma cell migration was largely mediated via \u03b1V\u03b23 integrin and inhibited by RGD peptides. Given the ubiquitous expression of \u03b15 laminins in melanoma cells and in melanoma-target tissues/anatomical structures, as well as the strong migration-promoting activity of these laminin isoforms, the \u03b15 laminins emerge as putative primary extracellular matrix mediators of melanoma invasion and metastasis via \u03b13\u03b21 and other integrin receptors.", "doi": "10.1016/j.yexcr.2010.12.019", "pmid": "21195710", "labels": {"National Genomics Infrastructure": null, "NGI Stockholm (Genomics Applications)": null, "NGI Stockholm (Genomics Production)": null}, "xrefs": [{"db": "pii", "key": "S0014-4827(10)00577-X"}], "notes": [], "created": "2017-05-04T14:57:18.577Z", "modified": "2020-01-21T13:56:01.105Z"}