{"entity": "publication", "iuid": "2034709ecac646fe9f1ac1a71faa2c00", "timestamp": "2026-07-17T05:02:52.460Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2034709ecac646fe9f1ac1a71faa2c00.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2034709ecac646fe9f1ac1a71faa2c00"}}, "title": "Structural basis of rapid actin dynamics in the evolutionarily divergent Leishmania parasite.", "authors": [{"family": "Kotila", "given": "Tommi", "initials": "T", "orcid": "0000-0002-9046-5834", "researcher": {"href": "https://publications.scilifelab.se/researcher/d530363e23ac4e3e8e24e7cdecfd0fe5.json"}}, {"family": "Wioland", "given": "Hugo", "initials": "H", "orcid": "0000-0001-5254-9642", "researcher": {"href": "https://publications.scilifelab.se/researcher/578f075141c245deb2f4c08702ef5090.json"}}, {"family": "Selvaraj", "given": "Muniyandi", "initials": "M", "orcid": "0000-0001-7793-6323", "researcher": {"href": "https://publications.scilifelab.se/researcher/1e74a48173fa4365897dd74ab6143c97.json"}}, {"family": "Kogan", "given": "Konstantin", "initials": "K", "orcid": "0000-0003-3270-9391", "researcher": {"href": "https://publications.scilifelab.se/researcher/1c6ce8d9a1474d5b9532650d2f5014d0.json"}}, {"family": "Antenucci", "given": "Lina", "initials": "L"}, {"family": "J\u00e9gou", "given": "Antoine", "initials": "A", "orcid": "0000-0003-0356-3127", "researcher": {"href": "https://publications.scilifelab.se/researcher/4e53291bfb224bfcb856be6e8ad06d9d.json"}}, {"family": "Huiskonen", "given": "Juha T", "initials": "JT", "orcid": "0000-0002-0348-7323", "researcher": {"href": "https://publications.scilifelab.se/researcher/c858a2e60971462f9e53653320c9eb0f.json"}}, {"family": "Romet-Lemonne", "given": "Guillaume", "initials": "G"}, {"family": "Lappalainen", "given": "Pekka", "initials": "P", "orcid": "0000-0001-6227-0354", "researcher": {"href": "https://publications.scilifelab.se/researcher/f9125bfd659d4e0784ce349140f84f5f.json"}}], "type": "journal article", "published": "2022-06-15", "journal": {"title": "Nat Commun", "issn": "2041-1723", "volume": "13", "issue": "1", "pages": "3442", "issn-l": "2041-1723"}, "abstract": "Actin polymerization generates forces for cellular processes throughout the eukaryotic kingdom, but our understanding of the 'ancient' actin turnover machineries is limited. We show that, despite > 1 billion years of evolution, pathogenic Leishmania major parasite and mammalian actins share the same overall fold and co-polymerize with each other. Interestingly, Leishmania harbors a simple actin-regulatory machinery that lacks cofilin 'cofactors', which accelerate filament disassembly in higher eukaryotes. By applying single-filament biochemistry we discovered that, compared to mammalian proteins, Leishmania actin filaments depolymerize more rapidly from both ends, and are severed > 100-fold more efficiently by cofilin. Our high-resolution cryo-EM structures of Leishmania ADP-, ADP-Pi- and cofilin-actin filaments identify specific features at actin subunit interfaces and cofilin-actin interactions that explain the unusually rapid dynamics of parasite actin filaments. Our findings reveal how divergent parasites achieve rapid actin dynamics using a remarkably simple set of actin-binding proteins, and elucidate evolution of the actin cytoskeleton.", "doi": "10.1038/s41467-022-31068-y", "pmid": "35705539", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9200798"}, {"db": "pii", "key": "10.1038/s41467-022-31068-y"}], "notes": [], "created": "2022-11-30T09:31:12.829Z", "modified": "2023-11-26T14:07:44.750Z"}