{"entity": "publication", "iuid": "1d494d4aae3148d6adc0f0e34b6fea33", "timestamp": "2026-07-15T10:06:19.829Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/1d494d4aae3148d6adc0f0e34b6fea33.json"}, "display": {"href": "https://publications.scilifelab.se/publication/1d494d4aae3148d6adc0f0e34b6fea33"}}, "title": "A link between the newly described colistin resistance gene mcr-9 and clinical Enterobacteriaceae isolates carrying blaSHV-12 from horses in Sweden.", "authors": [{"family": "B\u00f6rjesson", "given": "Stefan", "initials": "S", "orcid": "0000-0003-2219-2659", "researcher": {"href": "https://publications.scilifelab.se/researcher/e98c1d640f9a4eeaba4563eeec9e7dff.json"}}, {"family": "Greko", "given": "Christina", "initials": "C"}, {"family": "Myren\u00e5s", "given": "Mattias", "initials": "M"}, {"family": "Land\u00e9n", "given": "Annica", "initials": "A"}, {"family": "Nilsson", "given": "Oskar", "initials": "O"}, {"family": "Pedersen", "given": "Karl", "initials": "K"}], "type": "journal article", "published": "2020-03-00", "journal": {"title": "J Glob Antimicrob Resist", "issn": "2213-7173", "volume": "20", "issue": null, "pages": "285-289", "issn-l": "2213-7165"}, "abstract": "The aim of this study was to investigate the occurrence of the newly described transferable colistin resistance gene mcr-9 in extended-spectrum \u03b2-lactamase (ESBL)-producing clinical Enterobacteriaceae isolates from horses in Sweden.\n\nA total of 56 whole-genome sequenced ESBL-producing Enterobacteriaceae isolates from horses were subjected to in silico detection of antimicrobial resistance genes and identification of plasmid replicons types. The colistin minimum inhibitory concentration (MIC) for mcr-positive isolates was determined by broth microdilution. Relatedness between Enterobacteriaceae carrying mcr genes was determined by multilocus sequence typing (MLST) and core genome MLST.\n\nThirty ESBL-producing Enterobacteriaceae isolates from horses were positive for the colistin resistance gene mcr-9. These isolates included Enterobacter cloacae, Escherichia coli, Klebsiella oxytoca and Citrobacter freundii and belonged to diverse MLST sequence types within each species. Two of the mcr-9-containing isolates originated from the same horse. All mcr-9-positive isolates had colistin MICs below or equal to the EUCAST epidemiological cut-off value of 2 mg/L and were negative for the two potential regulatory genes qseB-like and qseC-like for mcr-9. Except for one isolate carrying only blaTEM-1B, all of the isolates carried blaSHV-12 and blaTEM-1B, and were all considered multidrug-resistant as they harboured genes encoding resistance to aminoglycosides, chloramphenicol, fosfomycin, macrolides, quinolones, sulfonamides, trimethoprim and tetracyclines. Plasmid replicon types IncHI2 and IncHI2A were detected in all mcr-9-positive isolates.\n\nThe occurrence of mcr-9 was common among clinical ESBL-producing Enterobacteriaceae isolates from horses in Sweden and was linked to the ESBL-encoding gene blaSHV-12 and plasmid replicon types IncHI2 and IncHI2A.", "doi": "10.1016/j.jgar.2019.08.007", "pmid": "31494305", "labels": {"Clinical Genomics Stockholm": "Service", "Bioinformatics Support for Computational Resources": "Service", "Clinical Genomics": "Service"}, "xrefs": [{"db": "pii", "key": "S2213-7165(19)30205-X"}], "notes": [], "created": "2020-11-22T14:28:37.132Z", "modified": "2024-01-16T13:48:42.885Z"}