{"entity": "publication", "iuid": "05430c40833b4be3b4985f41f27377d6", "timestamp": "2026-08-12T06:02:32.313Z", "links": {"self": {"href": "https://publications.scilifelab.se/publication/05430c40833b4be3b4985f41f27377d6.json"}, "display": {"href": "https://publications.scilifelab.se/publication/05430c40833b4be3b4985f41f27377d6"}}, "title": "Simultaneous visualization of DNA loci in single cells by combinatorial multi-color iFISH.", "authors": [{"family": "Mota", "given": "Ana", "initials": "A", "orcid": "0000-0001-8592-9764", "researcher": {"href": "https://publications.scilifelab.se/researcher/508960cbe03d4490a6e4594fc76e3510.json"}}, {"family": "Schweitzer", "given": "Maud", "initials": "M"}, {"family": "Wernersson", "given": "Erik", "initials": "E"}, {"family": "Crosetto", "given": "Nicola", "initials": "N", "orcid": "0000-0002-3019-6978", "researcher": {"href": "https://publications.scilifelab.se/researcher/bb66f0013e954d99a2be4df7309b7ae3.json"}}, {"family": "Bienko", "given": "Magda", "initials": "M"}], "type": "dataset", "published": "2022-02-10", "journal": {"title": "Sci Data", "issn": "2052-4463", "volume": "9", "issue": "1", "pages": "47", "issn-l": "2052-4463"}, "abstract": "Single-molecule DNA fluorescence in situ hybridization (FISH) techniques enable studying the three-dimensional (3D) organization of the genome at the single cell level. However, there is a major unmet need for open access, high quality, curated and reproducible DNA FISH datasets. Here, we describe a dataset obtained by applying our recently developed iFISH method to simultaneously visualize 16 small (size range: 62-73 kilobases, kb) DNA loci evenly spaced on chromosome 2 in human cells, in a single round of hybridization. We show how combinatorial color coding can be used to precisely localize multiple loci in 3D within single cells, and how inter-locus distances scale inversely with chromosome contact frequencies determined by high-throughput chromosome conformation capture (Hi-C). We provide raw images and 3D coordinates for nearly 10,000 FISH dots. Our dataset provides a free resource that can facilitate studies of 3D genome organization in single cells and can be used to develop automatic FISH analysis algorithms.", "doi": "10.1038/s41597-022-01139-2", "pmid": "35145120", "labels": {"Spatial Proteomics": "Technology development"}, "xrefs": [{"db": "pmc", "key": "PMC8831585"}, {"db": "pii", "key": "10.1038/s41597-022-01139-2"}], "notes": [], "created": "2023-06-26T12:49:04.953Z", "modified": "2023-06-26T12:49:05.083Z"}