{"entity": "journal", "iuid": "97cb0b86fedc40ee8445e2e2e6e45252", "timestamp": "2026-08-18T01:46:10.715Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Talanta.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Talanta"}}, "title": "Talanta", "issn": "1873-3573", "issn-l": null, "publications_count": 3, "publications": [{"entity": "publication", "iuid": "0f4c20c99096436cbbe67ed86c7cdf12", "links": {"self": {"href": "https://publications.scilifelab.se/publication/0f4c20c99096436cbbe67ed86c7cdf12.json"}, "display": {"href": "https://publications.scilifelab.se/publication/0f4c20c99096436cbbe67ed86c7cdf12"}}, "title": "Position specific isotope analysis of diethylamine by 2H and 13C NMR - Dual nucleus analysis in forensic investigation of illegal use of chemical weapons.", "authors": [{"family": "Lindberg", "given": "Sandra", "initials": "S"}, {"family": "Sparrman", "given": "Tobias", "initials": "T"}, {"family": "Schleucher", "given": "J\u00fcrgen", "initials": "J"}, {"family": "Norlin", "given": "Rikard", "initials": "R"}], "type": "journal article", "published": "2025-09-24", "journal": {"title": "Talanta", "issn": "1873-3573", "volume": "298", "issue": "Pt A", "pages": "128912", "issn-l": null}, "abstract": "Diethylamine (DEA) is a known precursor to some of the most toxic chemical warfare agents (CWA) as both V-agents and Fourth Generation Agents (FGAs) contain the dialkylamine functionality. DEA is also a readily available commercial substance with extensive use in the chemical industry for legitimate purposes. Because of this potential dual use, it is desirable to develop methods to trace the origin of dialkylamines if they are used for illicit purposes. We herein demonstrate that it is possible to differentiate six commercial batches of DEA using position-specific isotope analysis (PSIA) by both 2H and 13C NMR. Using a high-field NMR spectrometer together with a cryogenic 2H probe, we have produced 2H-{1H} NMR data with high accuracy and precision. The PSIA by NMR results show that the intramolecular 2H ratios of all six DEAs are significantly different while two of the six DEAs have unique 13C ratios. Further, the intramolecular isotopic variations can be used to link the DEAs to different suppliers. The two nuclei separately contribute to isotopic profiles of the DEAs. However, combining the two techniques provides a higher-resolved isotopic profile that can differentiate all DEAs and thus be useful in forensic investigations of illegal use of chemical weapons.", "doi": "10.1016/j.talanta.2025.128912", "pmid": "41022010", "labels": {"Swedish NMR Centre": "Collaborative"}, "xrefs": [{"db": "pii", "key": "S0039-9140(25)01403-1"}], "notes": [], "created": "2025-11-25T19:19:53.213Z", "modified": "2025-11-25T19:19:53.228Z"}, {"entity": "publication", "iuid": "2c3a844cdf4c4c3096e72bbe72e081c5", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2c3a844cdf4c4c3096e72bbe72e081c5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2c3a844cdf4c4c3096e72bbe72e081c5"}}, "title": "Quantitation of circulating short-chain fatty acids in small volume blood samples from animals and humans.", "authors": [{"family": "Fristedt", "given": "Rikard", "initials": "R"}, {"family": "Ruppert", "given": "Vanessa", "initials": "V"}, {"family": "Trower", "given": "Tania", "initials": "T"}, {"family": "Cooney", "given": "Janine", "initials": "J"}, {"family": "Landberg", "given": "Rikard", "initials": "R"}], "type": "journal article", "published": "2024-05-15", "journal": {"title": "Talanta", "issn": "1873-3573", "volume": "272", "pages": "125743", "issn-l": null}, "abstract": "The role of gut microbiota in human health has been intensively studied and more recently shifted from emphasis on composition towards function. Function is partly mediated through formed metabolites. Short-chain fatty acids (SCFAs) such as acetate, propionate, and butyrate as well as their branched analogues represent major products from gut fermentation of dietary fibre and proteins, respectively. Robust and high-throughput analysis of SCFAs in small volume blood samples have proven difficult. Major obstacles come from the ubiquitous presence of SCFAs that leads to contaminations and unstable analytical results because of the high volatility of these small molecules. Comprehensive and comparable data on the variation of SCFAs in blood samples from different blood matrices and mammal species including humans is lacking. Therefore, our aim was to develop and evaluate a stable and robust method for quantitation of 8 SCFAs and related fermentation products in small volume blood plasma samples and to investigate their variation in humans and different animal species.\n\nDerivatization was a successful approach for measurement of SCFAs in biological samples but quenching of the derivatization reaction was crucial to obtain long-term stability of the derivatized analytes. In total 9 compounds (including succinic acid) were separated in 5 min. The method was linear over the range 0.6-3200 nM formic (FA), acetic (AA), 0.3-1600 nM propionic (PA), and 0.16-800 nM for butyric (BA)-, isobutyric (IBA)-, valeric (VA)-, isovaleric (IVA)-, succinic (SA) and caproic acid (CA). The precision ranged \u226412 % within days and \u226428 % between days (except for CA and VA) in three different plasma quality control (QC) samples (29 batches analyzed over 3 months). The extraction recovery was on average 94 % for the different SCFAs. Typical interquartile range (IQR) concentrations (\u03bcM) of SCFAs in human plasma samples were 168 \u03bcM (FA), 64 \u03bcM (AA), 2.2 \u03bcM (PA), 0.54 \u03bcM (BA), 0.66 \u03bcM (IBA), 0.18 \u03bcM (VA), 0.40 \u03bcM (IVA), and 0.34 \u03bcM (CA). In total, 55 samples per batch/day were successfully analyzed and in total 5380 human plasma samples measured over a 3-year timespan.\n\nThe developed UHPLC-MS based method was suitable for measuring SCFAs in small blood volume samples and enabled robust quantitative data.", "doi": "10.1016/j.talanta.2024.125743", "pmid": "38382298", "labels": {"Chalmers Mass Spectrometry Infrastructure": "Collaborative"}, "xrefs": [{"db": "pii", "key": "S0039-9140(24)00122-X"}], "notes": [], "created": "2024-11-27T15:35:20.861Z", "modified": "2024-11-27T15:35:20.913Z"}, {"entity": "publication", "iuid": "d253ba8793ec401bbf89e56ecc5272fc", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d253ba8793ec401bbf89e56ecc5272fc.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d253ba8793ec401bbf89e56ecc5272fc"}}, "title": "Reagentless impedimetric immunosensor for monitoring of methotrexate in human blood serum using multiwalled carbon nanotube@polypyrrole/polytyramine film electrode.", "authors": [{"family": "Adeniyi", "given": "Kayode Omotayo", "initials": "KO"}, {"family": "Osmanaj", "given": "Blerina", "initials": "B"}, {"family": "Manavalan", "given": "Gopinathan", "initials": "G"}, {"family": "Mikkola", "given": "Jyri-Pekka", "initials": "JP"}, {"family": "Berisha", "given": "Avni", "initials": "A"}, {"family": "Tesfalidet", "given": "Solomon", "initials": "S"}], "type": "journal article", "published": "2024-02-01", "journal": {"title": "Talanta", "issn": "1873-3573", "volume": "268", "issue": "Pt 1", "pages": "125316", "issn-l": null}, "abstract": "Ensuring effective monitoring of methotrexate (MTX) levels in the bloodstream of cancer patients undergoing high-dose methotrexate chemotherapy is crucial to prevent potentially harmful side effects. However, the absence of portable analytical devices suitable for point-of-care bedside monitoring has presented a significant obstacle to achieving real-time MTX monitoring. In this study, we developed an impedimetric immunosensor that doesn't require reagents for measuring MTX levels in undiluted human blood serum. This reagentless approach simplifies the assay process, enabling rapid and straightforward MTX quantification. The immunosensor transducer was fabricated by electrodepositing conductive network of porous multiwalled carbon nanotube@polypyrrole/polytyramine on screen-printed gold microchip electrode (SP-Au/MWCNT70@PPy-PTA). Polyclonal anti-MTX antibodies were immobilized on the film, acting as the immunorecognition element. Non-specific binding was prevented by blocking the transducer interface with denatured bovine serum albumin (dBSA) fibrils, resulting in SP-Au/MWCNT70@PPy-PTA/anti-MTXAb|dBSA film electrode. When MTX binds to the SP-Au/MWCNT70@PPy-PTA/anti-MTXAb|dBSA interface, the film conductance and electron transfer resistance changes. This conductivity attenuation allows for electrochemical impedimetric signal transduction without a redox-probe solution. The electrochemical impedance spectroscopy (EIS) results showed increased charge transfer resistance and phase angle as MTX concentrations increased. The SP-Au/MWCNT70@PPy-PTA/anti-MTXAb|dBSA demonstrated high sensitivity, with a linear response from 0.02 to 20.0 \u03bcM and a detection limit of 1.93 nM. The detection limit was 50 times lower than the intended safe level of MTX in human serum. The immunosensor exhibited minimal cross-reactivity with endogenous MTX analogs and serum proteins. The SP-Au/MWCNT70@PPy-PTA/anti-MTXAb|dBSA immunosensor presents a simple and rapid method for therapeutic drug monitoring compared to traditional immunoassay systems.", "doi": "10.1016/j.talanta.2023.125316", "pmid": "37864856", "labels": {"Integrated Microscopy Technologies Ume\u00e5": "Service"}, "xrefs": [{"db": "pii", "key": "S0039-9140(23)01067-6"}], "notes": [], "created": "2024-11-07T14:55:47.311Z", "modified": "2024-11-07T14:55:47.398Z"}], "created": "2024-11-07T14:55:47.382Z", "modified": "2024-11-07T14:55:47.383Z"}