{"entity": "journal", "iuid": "8d4487f7ce834aada87a6ba0a8c52c01", "timestamp": "2026-07-21T08:07:51.572Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Pharmaceutics.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Pharmaceutics"}}, "title": "Pharmaceutics", "issn": "1999-4923", "issn-l": null, "publications_count": 2, "publications": [{"entity": "publication", "iuid": "b806633fe59244ba83201a73b0a8fe68", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b806633fe59244ba83201a73b0a8fe68.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b806633fe59244ba83201a73b0a8fe68"}}, "title": "Ginsenoside Rg3 Reduces the Toxicity of Graphene Oxide Used for pH-Responsive Delivery of Doxorubicin to Liver and Breast Cancer Cells.", "authors": [{"family": "Rahimi", "given": "Shadi", "initials": "S", "orcid": "0000-0002-8376-9880", "researcher": {"href": "https://publications.scilifelab.se/researcher/df63c5c027c448e290f3d00f0173844a.json"}}, {"family": "van Leeuwen", "given": "Daniel", "initials": "D"}, {"family": "Roshanzamir", "given": "Fariba", "initials": "F", "orcid": "0000-0002-2424-761X", "researcher": {"href": "https://publications.scilifelab.se/researcher/e3a055ea38ce4fb2a569eb5f801de7e7.json"}}, {"family": "Pandit", "given": "Santosh", "initials": "S", "orcid": "0000-0002-8357-758X", "researcher": {"href": "https://publications.scilifelab.se/researcher/ef2b4388693848c68cf42174ddeb65be.json"}}, {"family": "Shi", "given": "Lei", "initials": "L"}, {"family": "Sasanian", "given": "Nima", "initials": "N"}, {"family": "Nielsen", "given": "Jens", "initials": "J"}, {"family": "Esbj\u00f6rner", "given": "Elin K", "initials": "EK"}, {"family": "Mijakovic", "given": "Ivan", "initials": "I", "orcid": "0000-0002-8860-6853", "researcher": {"href": "https://publications.scilifelab.se/researcher/c0e5dfcc215b494a860ca6d9254b0b56.json"}}], "type": "journal article", "published": "2023-01-24", "journal": {"title": "Pharmaceutics", "issn": "1999-4923", "volume": "15", "issue": "2", "issn-l": null}, "abstract": "Doxorubicin (DOX) is extensively used in chemotherapy, but it has serious side effects and is inefficient against some cancers, e.g., hepatocarcinoma. To ameliorate the delivery of DOX and reduce its side effects, we designed a pH-responsive delivery system based on graphene oxide (GO) that is capable of a targeted drug release in the acidic tumor microenvironment. GO itself disrupted glutathione biosynthesis and induced reactive oxygen species (ROS) accumulation in human cells. It induced IL17-directed JAK-STAT signaling and VEGF gene expression, leading to increased cell proliferation as an unwanted effect. To counter this, GO was conjugated with the antioxidant, ginsenoside Rg3, prior to loading with DOX. The conjugation of Rg3 to GO significantly reduced the toxicity of the GO carrier by abolishing ROS production. Furthermore, treatment of cells with GO-Rg3 did not induce IL17-directed JAK-STAT signaling and VEGF gene expression-nor cell proliferation-suggesting GO-Rg3 as a promising drug carrier. The anticancer activity of GO-Rg3-DOX conjugates was investigated against Huh7 hepatocarcinoma and MDA-MB-231 breast cancer cells. GO-Rg3-DOX conjugates significantly reduced cancer cell viability, primarily via downregulation of transcription regulatory genes and upregulation of apoptosis genes. GO-Rg3 is an effective, biocompatible, and pH responsive DOX carrier with potential to improve chemotherapy-at least against liver and breast cancers.", "doi": "10.3390/pharmaceutics15020391", "pmid": "36839713", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9965446"}, {"db": "pii", "key": "pharmaceutics15020391"}], "notes": [], "created": "2023-12-01T10:50:28.523Z", "modified": "2023-12-01T10:50:28.649Z"}, {"entity": "publication", "iuid": "e6083a339dec4dd7bf5e5f04089797d7", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e6083a339dec4dd7bf5e5f04089797d7.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e6083a339dec4dd7bf5e5f04089797d7"}}, "title": "NanoSIMS Imaging Reveals the Impact of Ligand-ASO Conjugate Stability on ASO Subcellular Distribution.", "authors": [{"family": "Kay", "given": "Emma", "initials": "E", "orcid": "0000-0001-9668-6753", "researcher": {"href": "https://publications.scilifelab.se/researcher/0ff189b997464665bc014252804d7795.json"}}, {"family": "Stulz", "given": "Rouven", "initials": "R"}, {"family": "Becquart", "given": "C\u00e9cile", "initials": "C", "orcid": "0000-0002-7968-7129", "researcher": {"href": "https://publications.scilifelab.se/researcher/53c4dff8c3154c6884112040dd0469a0.json"}}, {"family": "Lovric", "given": "Jelena", "initials": "J"}, {"family": "T\u00e4ngemo", "given": "Carolina", "initials": "C"}, {"family": "Thomen", "given": "Aur\u00e9lien", "initials": "A"}, {"family": "Ba\u017edarevi\u0107", "given": "D\u017eenita", "initials": "D"}, {"family": "Najafinobar", "given": "Neda", "initials": "N"}, {"family": "Dahl\u00e9n", "given": "Anders", "initials": "A"}, {"family": "Pielach", "given": "Anna", "initials": "A"}, {"family": "Fernandez-Rodriguez", "given": "Julia", "initials": "J", "orcid": "0000-0003-4522-0966", "researcher": {"href": "https://publications.scilifelab.se/researcher/3bd9c743e99d479cb752aeec947acab3.json"}}, {"family": "Str\u00f6mberg", "given": "Roger", "initials": "R", "orcid": "0000-0002-7902-2688", "researcher": {"href": "https://publications.scilifelab.se/researcher/b76bd49836bf4c3eb0cfeac29ed6a238.json"}}, {"family": "\u00c4mm\u00e4l\u00e4", "given": "Carina", "initials": "C"}, {"family": "Andersson", "given": "Shalini", "initials": "S", "orcid": "0000-0002-7051-2189", "researcher": {"href": "https://publications.scilifelab.se/researcher/4bec6d49d3824027a9f1add9fc09588d.json"}}, {"family": "Kurczy", "given": "Michael", "initials": "M"}], "type": "journal article", "published": "2022-02-21", "journal": {"title": "Pharmaceutics", "issn": "1999-4923", "volume": "14", "issue": "2", "pages": "463", "issn-l": null}, "abstract": "The delivery of antisense oligonucleotides (ASOs) to specific cell types via targeted endocytosis is challenging due to the low cell surface expression of target receptors and inefficient escape of ASOs from the endosomal pathway. Conjugating ASOs to glucagon-like peptide 1 (GLP1) leads to efficient target knockdown, specifically in pancreatic \u03b2-cells. It is presumed that ASOs dissociate from GLP1 intracellularly to enable an ASO interaction with its target RNA. It is unknown where or when this happens following GLP1-ASO binding to GLP1R and endocytosis. Here, we use correlative nanoscale secondary ion mass spectroscopy (NanoSIMS) and transmission electron microscopy to explore GLP1-ASO subcellular trafficking in GLP1R overexpressing HEK293 cells. We isotopically label both eGLP1 and ASO, which do not affect the eGLP1-ASO conjugate function. We found that the eGLP1 peptide and ASO are not detected at the same level in the same endosomes, within 30 min of GLP1R-HEK293 cell exposure to eGLP1-ASO. When we utilized different linker chemistry to stabilize the GLP1-ASO conjugate, we observed more ASO located with GLP1 compared to cell incubation with the less stable conjugate. Overall, our work suggests that the ASO separates from GLP1 relatively early in the endocytic pathway, and that linker chemistry might impact the GLP1-ASO function.", "doi": "10.3390/pharmaceutics14020463", "pmid": "35214195", "labels": {"Integrated Microscopy Technologies Gothenburg": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC8876276"}, {"db": "pii", "key": "pharmaceutics14020463"}], "notes": [], "created": "2023-02-16T08:24:22.943Z", "modified": "2023-12-01T10:57:23.359Z"}], "created": "2023-02-16T08:24:23.082Z", "modified": "2023-02-16T08:24:23.082Z"}