{"entity": "journal", "iuid": "78dc0b09ef7340c4be25392fc4e148ac", "timestamp": "2026-07-20T22:37:40.219Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Pest%20Manag%20Sci.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Pest%20Manag%20Sci"}}, "title": "Pest Manag Sci", "issn": "1526-4998", "issn-l": null, "publications_count": 3, "publications": [{"entity": "publication", "iuid": "12c089c75858413289c4cd1d95bde9b4", "links": {"self": {"href": "https://publications.scilifelab.se/publication/12c089c75858413289c4cd1d95bde9b4.json"}, "display": {"href": "https://publications.scilifelab.se/publication/12c089c75858413289c4cd1d95bde9b4"}}, "title": "DMI fungicide resistance in Zymoseptoria tritici is unlinked to geographical origin and genetic background: a case study in Europe.", "authors": [{"family": "Oreiro", "given": "Eula Gems", "initials": "EG", "orcid": "0000-0001-9852-1549", "researcher": {"href": "https://publications.scilifelab.se/researcher/f4cbd2fbecbd461fa92ea04a5d9ab362.json"}}, {"family": "Samils", "given": "Berit", "initials": "B"}, {"family": "Kildea", "given": "Steven", "initials": "S", "orcid": "0000-0001-8240-6343", "researcher": {"href": "https://publications.scilifelab.se/researcher/e6329730a7674817aac8bb3662a658ca.json"}}, {"family": "Heick", "given": "Thies", "initials": "T"}, {"family": "Hellin", "given": "Pierre", "initials": "P", "orcid": "0000-0003-4777-6036", "researcher": {"href": "https://publications.scilifelab.se/researcher/0895796af24c4eca91bf783cc507f03c.json"}}, {"family": "Legr\u00e8ve", "given": "Anne", "initials": "A"}, {"family": "Rodemann", "given": "Bernd", "initials": "B"}, {"family": "Berg", "given": "Gunilla", "initials": "G"}, {"family": "J\u00f8rgensen", "given": "Lise N", "initials": "LN"}, {"family": "Friberg", "given": "Hanna", "initials": "H", "orcid": "0000-0003-3181-1597", "researcher": {"href": "https://publications.scilifelab.se/researcher/64ea9c2f457f46c48339ad375383475b.json"}}, {"family": "Berlin", "given": "Anna", "initials": "A"}, {"family": "Zhan", "given": "Jiasui", "initials": "J"}, {"family": "Andersson", "given": "Bj\u00f6rn", "initials": "B"}], "type": "journal article", "published": "2025-02-00", "journal": {"title": "Pest Manag Sci", "issn": "1526-4998", "volume": "81", "issue": "2", "pages": "1103-1112", "issn-l": null}, "abstract": "The hemibiotrophic fungus Zymoseptoria tritici causing Septoria tritici blotch (STB), is a devastating foliar pathogen of wheat worldwide. A common group of fungicides used to control STB are the demethylation inhibitors (DMIs). DMI fungicides restrict fungal growth by inhibiting the sterol 14-\u03b1-demethylase, a protein encoded by CYP51 gene and essential for maintaining fungal cell permeability. However, the adaptation of Z. tritici populations in response to intensive and prolonged DMI usage has resulted in a gradual shift towards reduced sensitivity to this group of fungicides. In this study, 311 isolates were collected pre-treatment from nine wheat-growing regions in Europe in 2019. These isolates were analysed by high-throughput amplicon-based sequencing of nine housekeeping genes and the CYP51 gene.\n\nAnalyses based on housekeeping genes and the CYP51 gene revealed a lack of population structure in Z. tritici samples irrespective of geographical origin. Minimum spanning network (MSN) analysis showed clustering of multilocus genotypes (MLGs) based on CYP51 haplotypes, indicating an effect of selection due to DMI fungicide use. The majority of the haplotypes identified in this study have been reported previously. The diversity and frequencies of mutations varied across regions.\n\nUsing a high-throughput amplicon-sequencing approach, we found several mutations in the CYP51 gene combined in different haplotypes that are likely to cause fungicide resistance. These mutations occurred irrespective of genetic background or geographical origin. Overall, these results contribute to the development of effective and sustainable risk monitoring for DMI fungicide resistance. \u00a9 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.", "doi": "10.1002/ps.8514", "pmid": "39503283", "labels": {"NGI Uppsala (Uppsala Genome Center)": "Service", "NGI Long read": "Service", "National Genomics Infrastructure": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11716363"}], "notes": [], "created": "2024-11-11T07:55:09.530Z", "modified": "2025-08-19T13:18:10.746Z"}, {"entity": "publication", "iuid": "7498c6b2bcfb4ad0aa9b57ed14b2b8a4", "links": {"self": {"href": "https://publications.scilifelab.se/publication/7498c6b2bcfb4ad0aa9b57ed14b2b8a4.json"}, "display": {"href": "https://publications.scilifelab.se/publication/7498c6b2bcfb4ad0aa9b57ed14b2b8a4"}}, "title": "Endophytic Beauveria bassiana induces biosynthesis of flavonoids in oilseed rape following both seed inoculation and natural colonization.", "authors": [{"family": "Muola", "given": "Anne", "initials": "A"}, {"family": "Birge", "given": "Traci", "initials": "T"}, {"family": "Helander", "given": "Marjo", "initials": "M"}, {"family": "Mathew", "given": "Suni", "initials": "S"}, {"family": "Harazinova", "given": "Vili", "initials": "V"}, {"family": "Saikkonen", "given": "Kari", "initials": "K"}, {"family": "Fuchs", "given": "Benjamin", "initials": "B", "orcid": "0000-0002-3678-3284", "researcher": {"href": "https://publications.scilifelab.se/researcher/c0bc0ab9ad0749b1b4ae4d6613e0c161.json"}}], "type": "journal article", "published": "2024-05-00", "journal": {"title": "Pest Manag Sci", "issn": "1526-4998", "volume": "80", "issue": "5", "pages": "2461-2470", "issn-l": null}, "abstract": "Cultivation of oilseed rape Brassica napus is pesticide-intensive, and alternative plant protection strategies are needed because both pesticide resistance and legislation narrow the range of effective chemical pesticides. The entomopathogenic fungus Beauveria bassiana is used as a biocontrol agent against various insect pests, but little is known about its endophytic potential and role in plant protection for oilseed rape. First, we studied whether B. bassiana can establish as an endophyte in oilseed rape, following seed inoculation. To evaluate the plant protection potential of endophytic B. bassiana on oilseed rape, we next examined its ability to induce plant metabolite biosynthesis. In another experiment, we tested the effect of seed inoculation on seedling survival in a semi-field experiment.\n\nBeauveria bassiana endophytically colonized oilseed rape following seed inoculation, and, in addition, natural colonization was also recorded. Maximum colonization rate was 40%, and generally increased with inoculation time. Seed inoculation did not affect the germination probability or growth of oilseed rape, but B. bassiana inoculated seeds germinated more slowly compared to controls. Endophytic colonization of B. bassiana induced biosynthesis of several flavonoids in oilseed rape leaves under controlled conditions. In the experiment conducted in semi-field conditions, inoculated seedlings had slightly higher mortality compared to control seedlings.\n\nBeauveria bassiana showed endophytic potential on oilseed rape via both natural colonization and seed inoculation, and it induced the biosynthesis of flavonoids. However, its use as an endophyte for plant protection against pests or pathogens for oilseed rape remains unclear. \u00a9 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.", "doi": "10.1002/ps.7672", "pmid": "37467342", "labels": {"Swedish Metabolomics Centre": "Service"}, "xrefs": [], "notes": [], "created": "2023-08-30T07:07:07.448Z", "modified": "2025-11-18T12:06:20.941Z"}, {"entity": "publication", "iuid": "692f75677a434eac84c073598d33eed7", "links": {"self": {"href": "https://publications.scilifelab.se/publication/692f75677a434eac84c073598d33eed7.json"}, "display": {"href": "https://publications.scilifelab.se/publication/692f75677a434eac84c073598d33eed7"}}, "title": "Bioproduction of (Z,E)-9,12-tetradecadienyl acetate (ZETA), the major pheromone component of Plodia, Ephestia, and Spodoptera species in yeast.", "authors": [{"family": "Ding", "given": "Bao-Jian", "initials": "BJ"}, {"family": "Wang", "given": "Hong-Lei", "initials": "HL"}, {"family": "Al-Saleh", "given": "Mohammed Ali", "initials": "MA"}, {"family": "L\u00f6fstedt", "given": "Christer", "initials": "C"}, {"family": "Antony", "given": "Binu", "initials": "B", "orcid": "0000-0002-6292-620X", "researcher": {"href": "https://publications.scilifelab.se/researcher/df53677d68fd42c1b557011462b73dde.json"}}], "type": "journal article", "published": "2022-03-00", "journal": {"title": "Pest Manag Sci", "issn": "1526-4998", "volume": "78", "issue": "3", "pages": "1048-1059", "issn-l": null}, "abstract": "(Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption, detection, monitoring, and mass trapping in raw and processed food storage facilities. In this study, we demonstrate the biological production of ZETA pheromone by engineered yeast Saccharomyces cerevisiae.\n\nWe mined the pheromone gland transcriptome data of the almond moth, Ephestia (Cadra) cautella (Walker), to trace a novel E12 fatty acyl desaturase and expressed candidates heterologously in yeast and Sf9 systems. Furthermore, we demonstrated a tailor-made ZETA pheromone bioproduction in yeast through metabolic engineering using this E12 desaturase, in combination with three genes from various sources coding for a Z9 desaturase, a fatty acyl reductase, and an acetyltransferase, respectively. Electrophysiological assays (gas chromatography coupled to an electroantennographic detector) proved that the transgenic yeast-produced ZETA pheromone component elicits distinct antennal responses.\n\nThe reconstructed biosynthetic pathway in yeast efficiently produces ZETA pheromone, leaves an undetectable level of biosynthetic intermediates, and paves the way for the economically competitive high-demand ZETA pheromone's bioproduction technology for high-value storage pest control.", "doi": "10.1002/ps.6716", "pmid": "34773383", "labels": {"Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC9300079"}], "notes": [], "created": "2022-11-09T15:51:20.620Z", "modified": "2024-01-16T13:48:37.381Z"}], "created": "2022-11-09T15:51:20.660Z", "modified": "2022-11-09T15:51:20.660Z"}