{"entity": "journal", "iuid": "a7655ed0d3784582afcbe3325213857d", "timestamp": "2026-07-15T16:04:40.620Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Pediatr%20Allergy%20Immunol.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Pediatr%20Allergy%20Immunol"}}, "title": "Pediatr Allergy Immunol", "issn": "1399-3038", "issn-l": "0905-6157", "publications_count": 6, "publications": [{"entity": "publication", "iuid": "f790d37b3e404a3cb7752afc90ae9af5", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f790d37b3e404a3cb7752afc90ae9af5.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f790d37b3e404a3cb7752afc90ae9af5"}}, "title": "Systemic inflammatory biomarkers in relation to lung function and exercise-induced bronchoconstriction in adolescents.", "authors": [{"family": "Ersson", "given": "Karin", "initials": "K", "orcid": "0000-0003-3185-3316", "researcher": {"href": "https://publications.scilifelab.se/researcher/d5f1f5fe2980458f9dd5dff19d5bc371.json"}}, {"family": "Alving", "given": "Kjell", "initials": "K"}, {"family": "Emtner", "given": "Margareta", "initials": "M"}, {"family": "Janson", "given": "Christer", "initials": "C"}, {"family": "Johansson", "given": "Henrik", "initials": "H"}, {"family": "Malinovschi", "given": "Andrei", "initials": "A", "orcid": "0000-0002-4098-7765", "researcher": {"href": "https://publications.scilifelab.se/researcher/7c246a24a09a4e67836f7ca6f1282610.json"}}], "type": "journal article", "published": "2025-10-00", "journal": {"title": "Pediatr Allergy Immunol", "issn": "1399-3038", "volume": "36", "issue": "10", "pages": "e70231", "issn-l": "0905-6157"}, "abstract": "The forced oscillation technique (FOT) complements spirometry in assessing lung function, with higher sensitivity to small airway dysfunction. Systemic inflammation is thought to influence lung development and exercise-induced bronchoconstriction (EIB), but its relationship to circulating inflammatory proteins in adolescents is unclear.\n\nTo investigate associations between systemic inflammatory biomarkers and baseline lung function and post-exercise airway responses in adolescents.\n\nIn 143 adolescents (13-15 years) from a population-based cohort, baseline spirometry, FOT, and baseline blood samples were obtained. Participants completed an exercise challenge to assess EIB via changes in forced expiratory volume in 1 s (FEV1), resistance at 5 Hz (R5), and reactance at 5 Hz (X5). Plasma protein levels were measured using the proximity extension assay technique (Olink Target Inflammation and Immune Response panels). Associations with lung function (FEV1% predicted, R5, and X5 z-scores) and post-exercise responses (\u2206FEV1, \u2206R5, \u2206X5) were analyzed using linear regression with false discovery rate correction. Interaction with atopy was also examined.\n\nHigher plasma levels of C-C motif chemokine 19 (CCL19) were significantly associated with lower FEV1% predicted and lower X5 z-scores at baseline, indicating reduced lung function and impaired small airway function. No proteins were associated with post-exercise airway responses after correction. Five proteins showed significant interactions with atopy in relation to EIB.\n\nElevated CCL19 may reflect systemic inflammatory processes contributing to impaired lung function in early adolescence. The observed atopy-related interactions suggest the need to consider atopy in studies of systemic inflammation and airway physiology.", "doi": "10.1111/pai.70231", "pmid": "41132128", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC12550650"}], "notes": [], "created": "2025-11-25T19:21:13.160Z", "modified": "2025-11-25T19:21:13.273Z"}, {"entity": "publication", "iuid": "e95cfadaa92444e5b0a63a2258e3b952", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e95cfadaa92444e5b0a63a2258e3b952.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e95cfadaa92444e5b0a63a2258e3b952"}}, "title": "Multiarray screening identifies plasma proteins associated with Th17 cell differentiation and viral defense in coincident asthma and obesity.", "authors": [{"family": "Manell", "given": "Hannes", "initials": "H", "orcid": "0000-0002-5209-4841", "researcher": {"href": "https://publications.scilifelab.se/researcher/97d8735a4595454bbb1c5d18435b97b3.json"}}, {"family": "Tsolakis", "given": "Nikolaos", "initials": "N"}, {"family": "Janson", "given": "Christer", "initials": "C"}, {"family": "Malinovschi", "given": "Andrei", "initials": "A"}, {"family": "Alving", "given": "Kjell", "initials": "K"}], "type": "journal article", "published": "2024-07-00", "journal": {"title": "Pediatr Allergy Immunol", "issn": "1399-3038", "volume": "35", "issue": "7", "pages": "e14187", "issn-l": "0905-6157"}, "abstract": "The immunological mechanisms behind the clinical association between asthma and obesity in adolescence are not fully understood. This study aimed to find new plasma protein biomarkers associated specifically with coincident asthma and obesity in adolescents.\n\nThis was a cross-sectional study in children and adolescents 10-19 years old (N = 390). Relative plasma concentrations of 113 protein biomarkers related to inflammation and immune response were determined by proximity extension assay (Target 96; Olink, Uppsala, Sweden). Differences in protein concentrations between healthy controls (n = 84), subjects with asthma (n = 138), subjects with obesity (n = 107), and subjects with both asthma and obesity (AO; n = 58) were analyzed by ANCOVA, adjusting for age and sex, and in a separate model adjusting also for the sum of specific IgE antibody concentrations to a mix of food allergens (fx5) and aeroallergens (Phadiatop). Proteins elevated in the AO group but not in the obesity or asthma groups were considered specifically elevated in asthma and obesity.\n\nFive proteins were elevated specifically in the AO group compared to controls (here sorted from largest to smallest effect of asthma and obesity combined): CCL8, IL-33, IL-17C, FGF-23, and CLEC7A. The effects of adjusting also for specific IgE were small but IL-33, IL-17C, and FGF-23 were no longer statistically significant.\n\nWe identified several new potential plasma biomarkers specifically elevated in coincident asthma and obesity in adolescents. Four of the proteins, CCL8, IL-33, IL-17C, and CLEC7A, have previously been associated with viral mucosal host defense and Th17 cell differentiation.", "doi": "10.1111/pai.14187", "pmid": "38967090", "labels": {"Affinity Proteomics Uppsala": "Service"}, "xrefs": [], "notes": [], "created": "2024-11-27T22:21:38.369Z", "modified": "2024-11-27T22:21:38.455Z"}, {"entity": "publication", "iuid": "055792514ea54dbaa757467d73ba8d5b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/055792514ea54dbaa757467d73ba8d5b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/055792514ea54dbaa757467d73ba8d5b"}}, "title": "Immune-related microRNAs in breast milk and their relation to regulatory T cells in breastfed children.", "authors": [{"family": "Ahlberg", "given": "Emelie", "initials": "E", "orcid": "0000-0002-7119-6114", "researcher": {"href": "https://publications.scilifelab.se/researcher/676300abed2d4948ba3e3937ca6c6e4a.json"}}, {"family": "Mart\u00ed", "given": "Magal\u00ed", "initials": "M", "orcid": "0000-0002-1927-4656", "researcher": {"href": "https://publications.scilifelab.se/researcher/6aad3e4e37b042c7a38e9f4717998410.json"}}, {"family": "Govindaraj", "given": "Dhanapal", "initials": "D", "orcid": "0000-0003-1303-8254", "researcher": {"href": "https://publications.scilifelab.se/researcher/8b619a8a42d74fb997fe908813c115ec.json"}}, {"family": "Severin", "given": "Elisabet", "initials": "E"}, {"family": "Duch\u00e9n", "given": "Karel", "initials": "K", "orcid": "0000-0002-0570-8898", "researcher": {"href": "https://publications.scilifelab.se/researcher/5784b47b03734e5292d3d0501a98a65c.json"}}, {"family": "Jenmalm", "given": "Maria C", "initials": "MC", "orcid": "0000-0002-2117-5366", "researcher": {"href": "https://publications.scilifelab.se/researcher/bf1f485192744e0c95ccecdb5471b577.json"}}, {"family": "Ting\u00f6", "given": "Lina", "initials": "L", "orcid": "0000-0002-2482-9281", "researcher": {"href": "https://publications.scilifelab.se/researcher/e2efcdbe9af6460290bf64e5d9843fcc.json"}}], "type": "randomized controlled trial", "published": "2023-04-00", "journal": {"title": "Pediatr Allergy Immunol", "issn": "1399-3038", "volume": "34", "issue": "4", "pages": "e13952", "issn-l": "0905-6157"}, "abstract": "The immunomodulatory capacity of breast milk may partially be mediated by microRNAs (miRNA), small RNA molecules that regulate gene expression on a post-transcriptional level and are hypothesized to be involved in modulation of immunological pathways. Here, we evaluate the expression of immune-related miRNAs in breast milk after pre- and postnatal supplementation with Limosilactobacillus reuteri and omega-3 (\u03c9-3) polyunsaturated fatty acids (PUFAs), and the association to infant regulatory T cell (Treg) frequencies.\n\nOne-hundred and twenty women included in a double-blind, randomized, placebo-controlled allergy intervention trial received L. reuteri and/or \u03c9-3 PUFAs daily from gestational week 20. Using Taqman qPCR, 24 miRNAs were analyzed from breast milk obtained at birth (colostrum) and after 3 months (mature milk) of lactation. The proportion of activated and resting Treg cells were analyzed in infant blood using flow cytometry at 6, 12, and 24 months.\n\nRelative expression changed significantly over the lactation period for most of the miRNAs; however, the expression was not significantly influenced by any of the supplements. Colostrum miR-181a-3p correlated with resting Treg cell frequencies at 6 months. Colostrum miR-148a-3p and let-7d-3p correlated with the frequencies of activated Treg cells at 24 months, as did mature milk miR-181a-3p and miR-181c-3p.\n\nMaternal supplementation with L. reuteri and \u03c9-3 PUFAs did not significantly affect the relative miRNA expression in breast milk. Interestingly, some of the miRNAs correlate with Treg subpopulations in the breastfed children, supporting the hypothesis that breast milk miRNAs could be important in infant immune regulation.\n\nClinicalTrials.gov-ID: NCT01542970.", "doi": "10.1111/pai.13952", "pmid": "37102392", "labels": {"Clinical Genomics Link\u00f6ping": "Service", "Clinical Genomics": "Service"}, "xrefs": [{"db": "ClinicalTrials.gov", "key": "NCT01542970"}], "notes": [], "created": "2023-12-03T05:45:47.197Z", "modified": "2023-12-03T05:45:47.376Z"}, {"entity": "publication", "iuid": "8e454ae8c8a04cc2874f7a2f09a423f9", "links": {"self": {"href": "https://publications.scilifelab.se/publication/8e454ae8c8a04cc2874f7a2f09a423f9.json"}, "display": {"href": "https://publications.scilifelab.se/publication/8e454ae8c8a04cc2874f7a2f09a423f9"}}, "title": "Virological and immunological features of SARS-COV-2 infected children with distinct symptomatology.", "authors": [{"family": "Cotugno", "given": "Nicola", "initials": "N", "orcid": "0000-0002-7748-1581", "researcher": {"href": "https://publications.scilifelab.se/researcher/56dc2dd7b83d4fbd9d20a008f89604da.json"}}, {"family": "Ruggiero", "given": "Alessandra", "initials": "A", "orcid": "0000-0002-1041-7489", "researcher": {"href": "https://publications.scilifelab.se/researcher/7f80953fcc4f42fabfc21a70d6a39aba.json"}}, {"family": "Pascucci", "given": "Giuseppe Rubens", "initials": "GR", "orcid": "0000-0002-5978-1193", "researcher": {"href": "https://publications.scilifelab.se/researcher/27e5b15541cb4305aacfbcdc76e8070c.json"}}, {"family": "Bonfante", "given": "Francesco", "initials": "F"}, {"family": "Petrara", "given": "Maria Raffaella", "initials": "MR"}, {"family": "Pighi", "given": "Chiara", "initials": "C"}, {"family": "Cifaldi", "given": "Loredana", "initials": "L"}, {"family": "Zangari", "given": "Paola", "initials": "P"}, {"family": "Bernardi", "given": "Stefania", "initials": "S"}, {"family": "Cursi", "given": "Laura", "initials": "L"}, {"family": "Santilli", "given": "Veronica", "initials": "V"}, {"family": "Manno", "given": "Emma Concetta", "initials": "EC"}, {"family": "Amodio", "given": "Donato", "initials": "D", "orcid": "0000-0003-4550-3018", "researcher": {"href": "https://publications.scilifelab.se/researcher/c1c02699a9564c09bff0a28036089857.json"}}, {"family": "Linardos", "given": "Giulia", "initials": "G"}, {"family": "Piccioni", "given": "Livia", "initials": "L"}, {"family": "Barbieri", "given": "Maria Antonietta", "initials": "MA"}, {"family": "Perrotta", "given": "Daniela", "initials": "D"}, {"family": "Campana", "given": "Andrea", "initials": "A"}, {"family": "Don\u00e0", "given": "Daniele", "initials": "D"}, {"family": "Giaquinto", "given": "Carlo", "initials": "C"}, {"family": "CACTUS Study Team", "given": "", "initials": ""}, {"family": "Concato", "given": "Carlo", "initials": "C"}, {"family": "Brodin", "given": "Petter", "initials": "P", "orcid": "0000-0002-8103-0046", "researcher": {"href": "https://publications.scilifelab.se/researcher/40097353cdb24e52bf2330eb687042bf.json"}}, {"family": "Rossi", "given": "Paolo", "initials": "P"}, {"family": "De Rossi", "given": "Anita", "initials": "A", "orcid": "0000-0001-6435-7509", "researcher": {"href": "https://publications.scilifelab.se/researcher/6ae101a8104840a09a3c965b2ad6d862.json"}}, {"family": "Palma", "given": "Paolo", "initials": "P", "orcid": "0000-0002-3066-4719", "researcher": {"href": "https://publications.scilifelab.se/researcher/275341cd978547cfa2aaf179cbd74011.json"}}], "type": "journal article", "published": "2021-11-00", "journal": {"title": "Pediatr Allergy Immunol", "issn": "1399-3038", "issn-l": "0905-6157", "volume": "32", "issue": "8", "pages": "1833-1842"}, "abstract": "Although SARS-CoV-2 immunizations have started in most countries, children are not currently included in the vaccination programs; thus, it remains crucial to define their anti-SARS-CoV-2 immune response in order to minimize the risk for other epidemic waves. This study sought to provide a description of the virology ad anti-SARS-CoV-2 immunity in children with distinct symptomatology.\n\nBetween March and July 2020, we recruited 15 SARS-CoV-2 asymptomatic (AS) and 51 symptomatic (SY) children, stratified according to WHO clinical classification. We measured SARS-CoV-2 viral load using ddPCR and qPCR in longitudinally collected nasopharyngeal swab samples. To define anti-SARS-CoV-2 antibodies, we measured neutralization activity and total IgG load (DiaSorin). We also evaluated antigen-specific B and CD8+T cells, using a labeled S1+S2 protein and ICAM expression, respectively. Plasma protein profiling was performed with Olink.\n\nVirological profiling showed that AS patients had lower viral load at diagnosis (p = .004) and faster virus clearance (p = .0002) compared with SY patients. Anti-SARS-CoV-2 humoral and cellular response did not appear to be associated with the presence of symptoms. AS and SY patients showed similar titers of SARS-CoV-2 IgG, levels of neutralizing activity, and frequency of Ag-specific B and CD8+ T cells, whereas pro-inflammatory plasma protein profile was found to be associated with symptomatology.\n\nWe demonstrated the development of anti-SARS-CoV-2 humoral and cellular response with any regard to symptomatology, suggesting the ability of both SY and AS patients to contribute toward herd immunity. The virological profiling of AS patients suggested that they have lower virus load associated with faster virus clearance.", "doi": "10.1111/pai.13585", "pmid": "34174102", "labels": {"Affinity Proteomics Stockholm": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC8420243"}], "notes": [], "created": "2022-07-11T08:29:44.391Z", "modified": "2023-06-19T11:10:16.918Z"}, {"entity": "publication", "iuid": "f005a36d6a6944bc939d90904088154d", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f005a36d6a6944bc939d90904088154d.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f005a36d6a6944bc939d90904088154d"}}, "title": "Pre- and postnatal Lactobacillus reuteri treatment alters DNA methylation of infant T helper cells.", "authors": [{"family": "Forsberg", "given": "Anna", "initials": "A"}, {"family": "Huoman", "given": "Johanna", "initials": "J", "orcid": "0000-0003-2509-2418", "researcher": {"href": "https://publications.scilifelab.se/researcher/471444d05e834a9b86a5a6d187da2c04.json"}}, {"family": "S\u00f6derholm", "given": "Simon", "initials": "S", "orcid": "0000-0001-5350-7102", "researcher": {"href": "https://publications.scilifelab.se/researcher/75f4744864904171b6a708e7a8d2b471.json"}}, {"family": "Bhai Mehta", "given": "Ratnesh", "initials": "R"}, {"family": "Nilsson", "given": "Lennart", "initials": "L", "orcid": "0000-0002-5680-6367", "researcher": {"href": "https://publications.scilifelab.se/researcher/19f74feeb54c4d588b879ea9e8843662.json"}}, {"family": "Abrahamsson", "given": "Thomas R", "initials": "TR"}, {"family": "Ernerudh", "given": "Jan", "initials": "J"}, {"family": "Gustafsson", "given": "Mika", "initials": "M"}, {"family": "Jenmalm", "given": "Maria C", "initials": "MC", "orcid": "0000-0002-2117-5366", "researcher": {"href": "https://publications.scilifelab.se/researcher/bf1f485192744e0c95ccecdb5471b577.json"}}], "type": "journal article", "published": "2020-07-00", "journal": {"title": "Pediatr Allergy Immunol", "issn": "1399-3038", "volume": "31", "issue": "5", "pages": "544-553", "issn-l": "0905-6157"}, "abstract": "Perinatal childhood exposures, including probiotic supplementation, may affect epigenetic modifications and impact on immune maturation and allergy development. The aim of this study was to assess the effects of pre- and postnatal Lactobacillus reuteri supplementation on DNA methylation in relation to immune maturation and allergy development.\n\nDNA methylation patterns were investigated for allergy-related T helper subsets using a locus-specific method and at a genome-wide scale using the Illumina 450K array. From a randomised, double-blind, placebo-controlled allergy prevention trial with pre- and postnatal probiotic supplementation, CD4+ T helper cells were obtained at birth (from cord blood), and 12 and 24 months of age (total (placebo/probiotics); locus-specific method: CB = 32 (17/15), 12 months = 24 (9/15), 24 months = 35 (15/20); Illumina: CB = 19 (10/9), 12 months = 10 (6/4), 24 months = 19(11/8)).\n\nComparing probiotics to placebo, the greatest genome-wide differential DNA methylation was observed at birth, where the majority of sites were hypomethylated, indicating transcriptional accessibility in the probiotic group. Bioinformatic analyses, including network analyses, revealed a module containing 91 genes, enriched for immune-related pathways such as chemotaxis, PI3K-Akt, MAPK and TGF-\u03b2 signalling. A majority of the module genes were associated with atopic manifestations (OR = 1.43, P = 2.4 \u00d7 10-6 ), and a classifier built on this model could predict allergy development (AUC = 0.78, P = 3.0 \u00d7 10e-3 ). Pathways such as IFN-\u03b3 signalling and T-cell activation were more hypermethylated at birth compared with later in life in both intervention groups over time, in line with DNA methylation patterns in the IFNG locus obtained by the locus-specific methodology.\n\nMaternal L. reuteri supplementation during pregnancy alters DNA methylation patterns in CD4+ T cells towards enhanced immune activation at birth, which may affect immune maturation and allergy development.", "doi": "10.1111/pai.13240", "pmid": "32150651", "labels": {"National Genomics Infrastructure": "Service", "NGI Uppsala (SNP&SEQ Technology Platform)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [], "notes": [], "created": "2020-03-12T15:01:36.562Z", "modified": "2024-01-16T13:48:42.269Z"}, {"entity": "publication", "iuid": "50e2dcce11a04e099509c3b4e9ae3bfe", "links": {"self": {"href": "https://publications.scilifelab.se/publication/50e2dcce11a04e099509c3b4e9ae3bfe.json"}, "display": {"href": "https://publications.scilifelab.se/publication/50e2dcce11a04e099509c3b4e9ae3bfe"}}, "title": "Late presenting atypical severe combined immunodeficiency (SCID) associated with a novel missense mutation in DCLRE1C.", "authors": [{"family": "Sundin", "given": "Mikael", "initials": "M"}, {"family": "Uhlin", "given": "Mikael", "initials": "M"}, {"family": "Gaballa", "given": "Ahmed", "initials": "A"}, {"family": "Ramme", "given": "Kim", "initials": "K"}, {"family": "Marits", "given": "Per", "initials": "P"}, {"family": "Nilsson", "given": "Jakob", "initials": "J"}], "type": "letter", "published": "2017-10-05", "journal": {"volume": null, "issn": "1399-3038", "issue": null, "title": "Pediatr Allergy Immunol", "issn-l": "0905-6157"}, "abstract": "Immunodeficiency associated with mutations in the DNA cross-link repair 1C gene (DCLRE1C) can have variable clinical presentations including severe combined immunodeficiency (SCID), Omenn syndrome, atypical SCID or common variable immunodeficiency (CVID) (1-3). DCLRE1C encodes the protein Artemis, a nuclease with intrinsic 5'-3' exonuclease activity on single-stranded DNA that is involved in non-homologous end joining (NHEJ). Artemis is essential for V(D)J recombination of the immunoglobulin and T-cell receptor genes that occur during B- and T-cell development.", "doi": "10.1111/pai.12812", "pmid": "28981982", "labels": {"Clinical Genomics Stockholm": "Service", "Clinical Genomics": "Service"}, "xrefs": [], "notes": [], "created": "2017-11-03T12:53:37.439Z", "modified": "2017-11-03T12:55:15.399Z"}], "created": "2017-11-03T12:53:37.463Z", "modified": "2020-11-27T13:14:02.279Z"}