{"entity": "journal", "iuid": "ae74eeea35d14d21888f655e3b49ce0d", "timestamp": "2026-07-20T12:53:35.516Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Nano%20Lett..json"}, "display": {"href": "https://publications.scilifelab.se/journal/Nano%20Lett."}}, "title": "Nano Lett.", "issn": "1530-6992", "issn-l": "1530-6984", "publications_count": 4, "publications": [{"entity": "publication", "iuid": "d424cf3383cb4f00b90f3c15f327aeae", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d424cf3383cb4f00b90f3c15f327aeae.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d424cf3383cb4f00b90f3c15f327aeae"}}, "title": "Dual-Color Expansion Microscopy of Membrane Proteins Using Bioorthogonal Labeling", "authors": [{"family": "Edwards", "given": "Steven", "initials": "S", "orcid": "0000-0001-7930-7977", "researcher": {"href": "https://publications.scilifelab.se/researcher/899ddd1ca8b148f696e5d30f30d762b1.json"}}, {"family": "Meineke", "given": "Birthe", "initials": "B", "orcid": "0000-0002-8912-0783", "researcher": {"href": "https://publications.scilifelab.se/researcher/e1cc783424be4fe2b41d82bba5c2a412.json"}}, {"family": "Bauer", "given": "Sebastian", "initials": "S", "orcid": "0009-0009-7265-1527", "researcher": {"href": "https://publications.scilifelab.se/researcher/b46eb06fbcd1476b8d55bd823a46a19c.json"}}, {"family": "Blom", "given": "Hans", "initials": "H", "orcid": "0000-0002-5584-9170", "researcher": {"href": "https://publications.scilifelab.se/researcher/3ce356a74dc84e0ea6af85397f11d869.json"}}, {"family": "Els\u00e4sser", "given": "Simon", "initials": "S", "orcid": "0000-0001-8724-4849", "researcher": {"href": "https://publications.scilifelab.se/researcher/fcf26e35e037499aa1441a7738ba61af.json"}}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications.scilifelab.se/researcher/1ec23336e2ef4e298f340876f1136dce.json"}}], "type": "journal-article", "published": "2026-02-04", "journal": {"title": "Nano Lett.", "issn": "1530-6984", "volume": "26", "issue": "4", "pages": "1321-1326", "issn-l": null}, "abstract": "With recent advances in fluorescence microscopy, resolution is often limited by the size of the label and the resulting linkage error, rather than the microscope itself. Site-specific incorporation of noncanonical amino acids (ncAAs) combined with bioorthogonal click chemistry provides a powerful tool for fluorescent protein labeling, overcoming the spatial uncertainty inherent to antibody-based probes. Here, we present a method to further improve labeling precision by combining ncAA labeling with expansion microscopy (ExM) for dual-color super-resolution imaging. After optimizing labeling procedures and fluorophore selection, we visualize and resolve the nanoscale distribution of Na,K-ATPase \u03b11 and \u03b21 subunits in expanded HEK 293T cells. We validate our approach by super-resolution STED imaging of the ncAA labeled \u03b21 subunit in unexpanded cells. This work presents a strong framework for multiplexed, high-resolution imaging, suggesting that ncAA labeling combined with ExM enables biological imaging at the nanometer scale.", "doi": "10.1021/acs.nanolett.5c05301", "pmid": "41571281", "labels": {"Integrated Microscopy Technologies Stockholm": "Technology development"}, "xrefs": [{"db": "pmc", "key": "PMC12879918"}], "notes": [], "created": "2026-01-24T20:15:09.343Z", "modified": "2026-02-11T12:54:50.277Z"}, {"entity": "publication", "iuid": "b69e3a293cb74fbc9c8c46ecf0a78fbb", "links": {"self": {"href": "https://publications.scilifelab.se/publication/b69e3a293cb74fbc9c8c46ecf0a78fbb.json"}, "display": {"href": "https://publications.scilifelab.se/publication/b69e3a293cb74fbc9c8c46ecf0a78fbb"}}, "title": "Imaging Single Particle Profiler to Study Nanoscale Bioparticles Using Conventional Confocal Microscopy.", "authors": [{"family": "Sych", "given": "Taras", "initials": "T", "orcid": "0000-0002-7330-9063", "researcher": {"href": "https://publications.scilifelab.se/researcher/d104a7d8096b4a4ab2a9fe618489fc7e.json"}}, {"family": "G\u00f6rgens", "given": "Andr\u00e9", "initials": "A", "orcid": "0000-0001-9198-0857", "researcher": {"href": "https://publications.scilifelab.se/researcher/ea5f85e2c23a4515a39a2fa23d665a99.json"}}, {"family": "Steiner", "given": "Lo\u00efc", "initials": "L", "orcid": "0000-0001-6640-3513", "researcher": {"href": "https://publications.scilifelab.se/researcher/ef6ac5c6f9324434b11fc1cccd0a9027.json"}}, {"family": "Gucluler", "given": "Gozde", "initials": "G"}, {"family": "Huge", "given": "Ylva", "initials": "Y"}, {"family": "Alamdari", "given": "Farhood", "initials": "F"}, {"family": "Johansson", "given": "Markus", "initials": "M"}, {"family": "Aljabery", "given": "Firas", "initials": "F"}, {"family": "Sherif", "given": "Amir", "initials": "A", "orcid": "0000-0002-3675-3050", "researcher": {"href": "https://publications.scilifelab.se/researcher/e27ad5d3def147849d4821d86075c3d2.json"}}, {"family": "Gabrielsson", "given": "Susanne", "initials": "S", "orcid": "0000-0003-1771-1346", "researcher": {"href": "https://publications.scilifelab.se/researcher/1d446cb52d7e4a01ad8478a9cfe22ae1.json"}}, {"family": "El Andaloussi", "given": "Samir", "initials": "S", "orcid": "0000-0003-4468-9113", "researcher": {"href": "https://publications.scilifelab.se/researcher/bd1036a42043441da3e444f4eac58010.json"}}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "journal article", "published": "2025-02-12", "journal": {"title": "Nano Lett.", "issn": "1530-6992", "volume": "25", "issue": "6", "pages": "2173-2180", "issn-l": "1530-6984"}, "abstract": "Single particle profiling (SPP) is a unique methodology to study nanoscale bioparticles such as liposomes, lipid nanoparticles, extracellular vesicles, and lipoproteins in a single particle and high throughput manner. The initial version requires the single photon counting modules for data acquisition, which limits its adoptability. Here, we present imaging-based SPP (iSPP) that can be performed by imaging a spot over time in the common imaging mode with confocal detectors. We also provide user-friendly software with a graphical user interface to analyze such data and give quantitative insights on the content and properties of nanoscale bioparticles. We use iSPP to decipher lipid-protein interactions, membrane modifications by drugs, and the heterogeneity of extracellular vesicles isolated from cell lines and human urine. This easily applicable modality of the single particle profiler will facilitate nanoscale bioparticle research in laboratories with access to any confocal microscope.", "doi": "10.1021/acs.nanolett.4c05117", "pmid": "39878336", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11827106"}], "notes": [], "created": "2025-03-11T12:50:21.409Z", "modified": "2025-03-24T08:21:15.353Z"}, {"entity": "publication", "iuid": "3557fe28e64c41ac899c296e03978ade", "links": {"self": {"href": "https://publications.scilifelab.se/publication/3557fe28e64c41ac899c296e03978ade.json"}, "display": {"href": "https://publications.scilifelab.se/publication/3557fe28e64c41ac899c296e03978ade"}}, "title": "A Multiparametric and High-Throughput Platform for Host\u2013Virus Binding Screens", "authors": [{"family": "Schlegel", "given": "Jan", "initials": "J", "orcid": "0000-0003-3159-8079", "researcher": {"href": "https://publications.scilifelab.se/researcher/c94351acc3934c3084f1ed0429a1879a.json"}}, {"family": "Porebski", "given": "Bartlomiej", "initials": "B"}, {"family": "Andronico", "given": "Luca", "initials": "L"}, {"family": "Hanke", "given": "Leo", "initials": "L"}, {"family": "Edwards", "given": "Steven", "initials": "S"}, {"family": "Brismar", "given": "Hjalmar", "initials": "H", "orcid": "0000-0003-0578-4003", "researcher": {"href": "https://publications.scilifelab.se/researcher/1ec23336e2ef4e298f340876f1136dce.json"}}, {"family": "Murrell", "given": "Ben", "initials": "B"}, {"family": "McInerney", "given": "Gerald M", "initials": "GM"}, {"family": "Fernandez-Capetillo", "given": "Oscar", "initials": "O"}, {"family": "Sezgin", "given": "Erdinc", "initials": "E", "orcid": "0000-0002-4915-388X", "researcher": {"href": "https://publications.scilifelab.se/researcher/34d3b05d68d64f698ff08dc655d2fe26.json"}}], "type": "journal-article", "published": "2023-05-10", "journal": {"title": "Nano Lett.", "issn": "1530-6984", "issn-l": null, "volume": "23", "issue": "9", "pages": "3701-3707"}, "abstract": null, "doi": "10.1021/acs.nanolett.2c04884", "pmid": null, "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [], "notes": [], "created": "2023-02-10T11:19:08.447Z", "modified": "2023-12-05T07:21:23.301Z"}, {"entity": "publication", "iuid": "1f477773d0a9429885c8dab2a73981b1", "links": {"self": {"href": "https://publications.scilifelab.se/publication/1f477773d0a9429885c8dab2a73981b1.json"}, "display": {"href": "https://publications.scilifelab.se/publication/1f477773d0a9429885c8dab2a73981b1"}}, "title": "Peptide Functionalized Gold Nanoparticles as a Stimuli Responsive Contrast Medium in Multiphoton Microscopy.", "authors": [{"family": "Borglin", "given": "Johan", "initials": "J"}, {"family": "Seleg\u00e5rd", "given": "Robert", "initials": "R"}, {"family": "Aili", "given": "Daniel", "initials": "D", "orcid": "0000-0002-7001-9415", "researcher": {"href": "https://publications.scilifelab.se/researcher/cc760610215a4dee9061f28723b97e62.json"}}, {"family": "Ericson", "given": "Marica B", "initials": "MB", "orcid": "0000-0002-5987-5915", "researcher": {"href": "https://publications.scilifelab.se/researcher/af1f53e7cd234c36bdde7121e7204ed5.json"}}], "type": "journal article", "published": "2017-03-08", "journal": {"title": "Nano Lett.", "issn": "1530-6992", "volume": "17", "issue": "3", "pages": "2102-2108", "issn-l": "1530-6984"}, "abstract": "There is a need for biochemical contrast mediators with high signal-to-noise ratios enabling noninvasive biomedical sensing, for example, for neural sensing and protein-protein interactions, in addition to cancer diagnostics. The translational challenge is to develop a biocompatible approach ensuring high biochemical contrast while avoiding a raise of the background signal. We here present a concept where gold nanoparticles (AuNPs) can be utilized as a stimuli responsive contrast medium by chemically triggering their ability to exhibit multiphoton-induced luminescence (MIL) when performing multiphoton laser scanning microscopy (MPM). Proof-of-principle is demonstrated using peptide-functionalized AuNPs sensitive to zinc ions (Zn 2+). Dispersed particles are invisible in the MPM until addition of millimolar concentrations of Zn2+ upon which MIL is enabled through particle aggregation caused by specific peptide interactions and folding. The process can be reversed by removal of the Zn2+ using a chelator, thereby resuspending the AuNPs. In addition, the concept was demonstrated by exposing the particles to matrix metalloproteinase-7 (MMP-7) causing peptide digestion resulting in AuNP aggregation, significantly elevating the MIL signal from the background. The approach is based on the principle that aggregation shifts the plasmon resonance, elevating the absorption cross section in the near-infrared wavelength region enabling onset of MIL. This Letter demonstrates how biochemical sensing can be obtained in far-field MPM and should be further exploited as a future tool for noninvasive optical biosensing.", "doi": "10.1021/acs.nanolett.7b00611", "pmid": "28215085", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [], "notes": [], "created": "2020-01-23T16:34:34.853Z", "modified": "2021-06-21T15:41:57.977Z"}], "created": "2020-01-23T16:34:34.867Z", "modified": "2020-11-27T13:14:04.724Z"}