{"entity": "journal", "iuid": "d707e797d6164884b7f890058b606577", "timestamp": "2026-07-13T10:14:01.313Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Mucosal%20Immunol.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Mucosal%20Immunol"}}, "title": "Mucosal Immunol", "issn": "1935-3456", "issn-l": "1933-0219", "publications_count": 6, "publications": [{"entity": "publication", "iuid": "ff9cb80f60b040cfa8d506bea536c913", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ff9cb80f60b040cfa8d506bea536c913.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ff9cb80f60b040cfa8d506bea536c913"}}, "title": "A zebrafish model of intestinal epithelial damage reveals macrophages and igfbp1a as major modulators of mucosal healing.", "authors": [{"family": "Morales Castro", "given": "Rodrigo A", "initials": "RA"}, {"family": "Kern", "given": "Bianca C", "initials": "BC"}, {"family": "D\u00edaz-Basabe", "given": "Ang\u00e9lica", "initials": "A"}, {"family": "Meinen", "given": "Eveline R", "initials": "ER"}, {"family": "Zhao", "given": "Danxia", "initials": "D"}, {"family": "Zhou", "given": "Yuqing", "initials": "Y"}, {"family": "Castillo", "given": "Francisca", "initials": "F"}, {"family": "Monasterio", "given": "Gustavo", "initials": "G"}, {"family": "Farcas", "given": "Vlad", "initials": "V"}, {"family": "Ch\u00e1vez", "given": "Myra N", "initials": "MN"}, {"family": "Fransson", "given": "Jennifer", "initials": "J"}, {"family": "Villablanca", "given": "Eduardo J", "initials": "EJ"}], "type": "journal article", "published": "2025-04-17", "journal": {"title": "Mucosal Immunol", "issn": "1935-3456", "issn-l": "1933-0219"}, "abstract": "Promoting intestinal regeneration and enhancing mucosal healing have emerged as promising therapeutic alternatives for treating intestinal disorders that compromise epithelial barrier integrity and function. However, the cellular and molecular mechanisms underlying these processes remain poorly understood. This knowledge gap is partly due to the lack of reliable and cost-effective in vivo models for studying the mechanisms governing intestinal damage and regeneration. Here, we developed a controlled, inducible, and targeted intestinal epithelial cell (IEC) ablation transgenic zebrafish model that recapitulates features of intestinal damage and regeneration observed in humans. Single-cell RNAseq and live imaging revealed accumulation of macrophages in the recovering intestine, contributing to its regeneration. Furthermore, we observed overexpression of insulin-like growth factor binding protein 1a (igfbp1a) during intestinal damage. Morpholino-mediated knockdown of igfbp1a exacerbated intestinal damage and impaired subsequent regeneration. In summary, we introduced a novel zebrafish model of intestinal damage that enables in vivo high-throughput screening for identifying and validating novel modulators of mucosal healing and intestinal regeneration.", "doi": "10.1016/j.mucimm.2025.04.004", "pmid": "40252728", "labels": {"Integrated Microscopy Technologies Stockholm": "Service"}, "xrefs": [{"db": "pii", "key": "S1933-0219(25)00042-X"}], "notes": [], "created": "2025-08-01T08:44:14.561Z", "modified": "2025-08-01T08:44:14.567Z"}, {"entity": "publication", "iuid": "ecc798da00bd489298e00c1eb42e9dad", "links": {"self": {"href": "https://publications.scilifelab.se/publication/ecc798da00bd489298e00c1eb42e9dad.json"}, "display": {"href": "https://publications.scilifelab.se/publication/ecc798da00bd489298e00c1eb42e9dad"}}, "title": "Migratory CD103+CD11b+ cDC2s in Peyer's patches are critical for gut IgA responses following oral immunization.", "authors": [{"family": "Gribonika", "given": "Inta", "initials": "I"}, {"family": "Str\u00f6mberg", "given": "Anneli", "initials": "A"}, {"family": "Chandode", "given": "Rakesh K", "initials": "RK"}, {"family": "Sch\u00f6n", "given": "Karin", "initials": "K"}, {"family": "Lahl", "given": "Katharina", "initials": "K"}, {"family": "Bemark", "given": "Mats", "initials": "M"}, {"family": "Lycke", "given": "Nils", "initials": "N"}], "type": "journal article", "published": "2024-08-00", "journal": {"title": "Mucosal Immunol", "issn": "1935-3456", "volume": "17", "issue": "4", "pages": "509-523", "issn-l": "1933-0219"}, "abstract": "Induction and regulation of specific intestinal immunoglobulin (Ig)A responses critically depend on dendritic cell (DC) subsets and the T cells they activate in the Peyer's patches (PP). We found that oral immunization with cholera toxin (CT) as an adjuvant resulted in migration-dependent changes in the composition and localization of PP DC subsets with increased numbers of cluster of differentiation (CD)103- conventional DC (cDC)2s and lysozyme-expressing DC (LysoDCs) in the subepithelial dome and of CD103+ cDC2s that expressed CD101 in the T cell zones, while oral ovalbumin (OVA) tolerization was instead associated with greater accumulation of cDC1s and peripherally induced regulatory T cells (pTregs) in this area. Decreased IgA responses were observed after CT-adjuvanted immunization in huCD207DTA mice lacking CD103+ cDC2s, while oral OVA tolerization was inefficient in cDC1-deficient Batf3-/- mice. Using OVA transgenic T cell receptor CD4 T cell adoptive transfer models, we found that co-transferred endogenous wildtype CD4 T cells can hinder the induction of OVA-specific IgA responses through secretion of interleukin-10. CT could overcome this blocking effect, apparently through a modulating effect on pTregs while promoting an expansion of follicular helper T cells. The data support a model where cDC1-induced pTreg normally suppresses PP responses for any given antigen and where CT's oral adjuvanticity effect is dependent on promoting follicular helper T cell responses through induction of CD103+ cDC2s.", "doi": "10.1016/j.mucimm.2024.03.004", "pmid": "38492746", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pii", "key": "S1933-0219(24)00023-0"}], "notes": [], "created": "2024-11-15T12:05:07.359Z", "modified": "2024-11-15T12:05:07.363Z"}, {"entity": "publication", "iuid": "f9e35459e6904c19b9f2d12e3cb5b023", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f9e35459e6904c19b9f2d12e3cb5b023.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f9e35459e6904c19b9f2d12e3cb5b023"}}, "title": "Interleukin-10 regulates goblet cell numbers through Notch signaling in the developing zebrafish intestine.", "authors": [{"family": "Morales", "given": "Rodrigo A", "initials": "RA"}, {"family": "Rabahi", "given": "Soraya", "initials": "S"}, {"family": "Diaz", "given": "Oscar E", "initials": "OE"}, {"family": "Salloum", "given": "Yazan", "initials": "Y"}, {"family": "Kern", "given": "Bianca C", "initials": "BC"}, {"family": "Westling", "given": "Mikaela", "initials": "M"}, {"family": "Luo", "given": "Xinxin", "initials": "X"}, {"family": "Parigi", "given": "Sara M", "initials": "SM"}, {"family": "Monasterio", "given": "Gustavo", "initials": "G"}, {"family": "Das", "given": "Srustidhar", "initials": "S"}, {"family": "Hern\u00e1ndez", "given": "Pedro P", "initials": "PP"}, {"family": "Villablanca", "given": "Eduardo J", "initials": "EJ", "orcid": "0000-0001-9522-9729", "researcher": {"href": "https://publications.scilifelab.se/researcher/6c6a2dde2d8f40ef82dfba0cf1b52c0d.json"}}], "type": "journal article", "published": "2022-05-00", "journal": {"title": "Mucosal Immunol", "issn": "1935-3456", "volume": "15", "issue": "5", "pages": "940-951", "issn-l": "1933-0219"}, "abstract": "Cytokines are immunomodulatory proteins that orchestrate cellular networks in health and disease. Among these, interleukin (IL)-10 is critical for the establishment of intestinal homeostasis, as mutations in components of the IL-10 signaling pathway result in spontaneous colitis. Whether IL-10 plays other than immunomodulatory roles in the intestines is poorly understood. Here, we report that il10, il10ra, and il10rb are expressed in the zebrafish developing intestine as early as 3 days post fertilization. CRISPR/Cas9-generated il10-deficient zebrafish larvae showed an increased expression of pro-inflammatory genes and an increased number of intestinal goblet cells compared to WT larvae. Mechanistically, Il10 promotes Notch signaling in zebrafish intestinal epithelial cells, which in turn restricts goblet cell expansion. Using murine organoids, we showed that IL-10 modulates goblet cell frequencies in mammals, suggesting conservation across species. This study demonstrates a previously unappreciated IL-10-Notch axis regulating goblet cell homeostasis in the developing zebrafish intestine and may help explain the disease severity of IL-10 deficiency in the intestines of mammals.", "doi": "10.1038/s41385-022-00546-3", "pmid": "35840681", "labels": {"Genome Engineering Zebrafish": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41385-022-00546-3"}, {"db": "pmc", "key": "PMC9385495"}], "notes": [], "created": "2022-08-24T08:40:52.090Z", "modified": "2022-08-24T08:40:52.113Z"}, {"entity": "publication", "iuid": "2f54b302697c4891b3ea172032b6c3ac", "links": {"self": {"href": "https://publications.scilifelab.se/publication/2f54b302697c4891b3ea172032b6c3ac.json"}, "display": {"href": "https://publications.scilifelab.se/publication/2f54b302697c4891b3ea172032b6c3ac"}}, "title": "Clonotypic analysis of protective influenza M2e-specific lung resident Th17 memory cells reveals extensive functional diversity", "authors": [{"family": "Omokanye", "given": "Ajibola", "initials": "A"}, {"family": "Ong", "given": "Li Ching", "initials": "LC"}, {"family": "Lebrero-Fernandez", "given": "Cristina", "initials": "C"}, {"family": "Bernasconi", "given": "Valentina", "initials": "V"}, {"family": "Sch\u00f6n", "given": "Karin", "initials": "K"}, {"family": "Str\u00f6mberg", "given": "Anneli", "initials": "A"}, {"family": "Bemark", "given": "Mats", "initials": "M"}, {"family": "Saelens", "given": "Xavier", "initials": "X"}, {"family": "Czarnewski", "given": "Paulo", "initials": "P"}, {"family": "Lycke", "given": "Nils", "initials": "N", "orcid": "0000-0003-1155-4861", "researcher": {"href": "https://publications.scilifelab.se/researcher/cc624dbe15d24b8a90ad8d8673ffd390.json"}}], "type": "journal-article", "published": "2022-04-00", "journal": {"title": "Mucosal Immunol", "issn": "1933-0219", "volume": "15", "issue": "4", "pages": "717-729", "issn-l": null}, "abstract": "The fate of tissue-resident memory CD4 T cells (Trm) has been incompletely investigated. Here we show that intranasal, but not parenteral, immunization with CTA1-3M2e-DD stimulated M2e-specific Th17 Trm cells, which conferred strong protection against influenza virus infection in the lung. These cells rapidly expanded upon infection and effectively restricted virus replication as determined by CD4 T cell depletion studies. Single-cell RNAseq transcriptomic and TCR VDJ-analysis of M2e-tetramer-sorted CD4 T cells on day 3 and 8 post infection revealed complete Th17-lineage dominance (no Th1 or Tregs) with extensive functional diversity and expression of gene markers signifying mature resident Trm cells (Cd69, Nfkbid, Brd2, FosB). Unexpectedly, the same TCR clonotype hosted cells with different Th17 subcluster functions (IL-17, IL-22), regulatory and cytotoxic cells, suggesting a tissue and context-dependent differentiation of reactivated Th17 Trm cells. A gene set enrichment analysis demonstrated up-regulation of regulatory genes (Lag3, Tigit, Ctla4, Pdcd1) in M2e-specific Trm cells on day 8, indicating a tissue damage preventing function. Thus, contrary to current thinking, lung M2e-specific Th17 Trm cells are sufficient for controlling infection and for protecting against tissue injury. These findings will have strong implications for vaccine development against respiratory virus infections and influenza virus infections, in particular.", "doi": "10.1038/s41385-022-00497-9", "pmid": "35260804", "labels": {"Bioinformatics Support, Infrastructure and Training": "Collaborative", "Bioinformatics Long-term Support WABI": "Collaborative", "Bioinformatics (NBIS)": "Collaborative"}, "xrefs": [{"db": "pii", "key": "10.1038/s41385-022-00497-9"}, {"db": "pmc", "key": "PMC8903128"}], "notes": [], "created": "2022-04-04T19:19:42.737Z", "modified": "2023-04-26T11:54:18.956Z"}, {"entity": "publication", "iuid": "d36c2b0a3e7e445991d019d2c87c528b", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d36c2b0a3e7e445991d019d2c87c528b.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d36c2b0a3e7e445991d019d2c87c528b"}}, "title": "ADP-ribosylating adjuvant reveals plasticity in cDC1 cells that drive mucosal Th17 cell development and protection against influenza virus infection.", "authors": [{"family": "Arabpour", "given": "Mohammad", "initials": "M"}, {"family": "Lebrero-Fernandez", "given": "Cristina", "initials": "C"}, {"family": "Sch\u00f6n", "given": "Karin", "initials": "K"}, {"family": "Str\u00f6mberg", "given": "Anneli", "initials": "A"}, {"family": "B\u00f6rjesson", "given": "Vanja", "initials": "V"}, {"family": "Lahl", "given": "Katharina", "initials": "K"}, {"family": "Ballegeer", "given": "Marlies", "initials": "M"}, {"family": "Saelens", "given": "Xavier", "initials": "X"}, {"family": "Angeletti", "given": "Davide", "initials": "D"}, {"family": "Agace", "given": "William", "initials": "W"}, {"family": "Lycke", "given": "Nils", "initials": "N", "orcid": "0000-0003-1155-4861", "researcher": {"href": "https://publications.scilifelab.se/researcher/cc624dbe15d24b8a90ad8d8673ffd390.json"}}], "type": "journal article", "published": "2022-04-00", "journal": {"title": "Mucosal Immunol", "issn": "1935-3456", "issn-l": "1933-0219", "volume": "15", "issue": "4", "pages": "745-761"}, "abstract": "Migratory dendritic cells expressing CD103 are the targets for mucosal vaccines. These belong to either of two lineage-restricted subsets, cDC1 or cDC2 cells, which have been linked to priming of functionally distinct CD4 T cells. However, recent studies have identified plasticity in cDC2 cells with overlapping functions with cDC1 cells, while the converse has not been reported. We genetically engineered a vaccine adjuvant platform that targeted the cholera toxin A1 (CTA1) ADP-ribosylating enzyme to CD103+ cDC1 and cDC2 cells using a single-chain antibody (scFv) to CD103. Unexpectedly, intranasal immunization with the CTA1-svFcCD103 adjuvant modified cDC1 cells to effectively prime Th17 cells, a function previously limited to cDC2 cells. In fact, cDC2 cells were dispensible, while cDC1 cells, lacking in Batf3-/- mice, were critical. Following intranasal immunizations isolated cDC1 cells from mLN exclusively promoted Rorgt+ T cells and IL-17, IL-21, and IL-22 production. Strong CD8 T cell responses through antigen cross presentation by cDC1 cells were also observed. Single-cell RNAseq analysis revealed upregulation of Th17-promoting gene signatures in sorted cDC1 cells. Gene expression in isolated cDC2 cells was largely unaffected. Our finding represents a major shift of paradigm as we have documented functional plasticity in cDC1 cells.", "doi": "10.1038/s41385-022-00510-1", "pmid": "35418673", "labels": {"Clinical Genomics Gothenburg": "Collaborative", "Clinical Genomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC9259495"}, {"db": "pii", "key": "10.1038/s41385-022-00510-1"}], "notes": [], "created": "2022-12-02T12:23:18.797Z", "modified": "2023-05-15T16:36:15.231Z"}, {"entity": "publication", "iuid": "f843775d10b4408690827760cc7f1627", "links": {"self": {"href": "https://publications.scilifelab.se/publication/f843775d10b4408690827760cc7f1627.json"}, "display": {"href": "https://publications.scilifelab.se/publication/f843775d10b4408690827760cc7f1627"}}, "title": "Helicobacter suis infection alters glycosylation and decreases the pathogen growth inhibiting effect and binding avidity of gastric mucins.", "authors": [{"family": "Padra", "given": "M\u00e9dea", "initials": "M"}, {"family": "Adamczyk", "given": "Barbara", "initials": "B"}, {"family": "Flahou", "given": "Bram", "initials": "B"}, {"family": "Erhardsson", "given": "Mattias", "initials": "M"}, {"family": "Chahal", "given": "Gurdeep", "initials": "G"}, {"family": "Smet", "given": "Annemieke", "initials": "A"}, {"family": "Jin", "given": "Chunsheng", "initials": "C"}, {"family": "Thorell", "given": "Anders", "initials": "A"}, {"family": "Ducatelle", "given": "Richard", "initials": "R"}, {"family": "Haesebrouck", "given": "Freddy", "initials": "F"}, {"family": "Karlsson", "given": "Niclas G", "initials": "NG"}, {"family": "Lind\u00e9n", "given": "Sara K", "initials": "SK"}], "type": "journal article", "published": "2019-05-00", "journal": {"title": "Mucosal Immunol", "issn": "1935-3456", "volume": "12", "issue": "3", "pages": "784-794", "issn-l": "1933-0219"}, "abstract": "Helicobacter suis is the most prevalent non-Helicobacter pylori Helicobacter species in the human stomach and is associated with chronic gastritis, peptic ulcer disease, and gastric mucosa-associated lymphoid tissue (MALT) lymphoma. H. suis colonizes the gastric mucosa of 60-95% of pigs at slaughter age, and is associated with chronic gastritis, decreased weight gain, and ulcers. Here, we show that experimental H. suis infection changes the mucin composition and glycosylation, decreasing the amount of H. suis-binding glycan structures in the pig gastric mucus niche. Similarly, the H. suis-binding ability of mucins from H. pylori-infected humans is lower than that of noninfected individuals. Furthermore, the H. suis growth-inhibiting effect of mucins from both noninfected humans and pigs is replaced by a growth-enhancing effect by mucins from infected individuals/pigs. Thus, Helicobacter spp. infections impair the mucus barrier by decreasing the H. suis-binding ability of the mucins and by decreasing the antiprolific activity that mucins can have on H. suis. Inhibition of these mucus-based defenses creates a more stable and inhabitable niche for H. suis. This is likely of importance for long-term colonization and outcome of infection, and reversing these impairments may have therapeutic benefits.", "doi": "10.1038/s41385-019-0154-4", "pmid": "30846831", "labels": {"Glycoproteomics and MS Proteomics": "Service"}, "xrefs": [{"db": "pii", "key": "10.1038/s41385-019-0154-4"}], "notes": [], "created": "2020-01-30T16:24:37.560Z", "modified": "2024-01-16T13:46:31.819Z"}], "created": "2020-01-30T16:24:37.574Z", "modified": "2020-11-27T13:14:08.194Z"}