{"entity": "journal", "iuid": "33809b0210d44c1db880551ff794236b", "timestamp": "2026-07-22T17:11:17.607Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Mol.%20Hum.%20Reprod..json"}, "display": {"href": "https://publications.scilifelab.se/journal/Mol.%20Hum.%20Reprod."}}, "title": "Mol. Hum. Reprod.", "issn": "1460-2407", "issn-l": "1360-9947", "publications_count": 3, "publications": [{"entity": "publication", "iuid": "72e9b85908ed435d8265e945883e4b04", "links": {"self": {"href": "https://publications.scilifelab.se/publication/72e9b85908ed435d8265e945883e4b04.json"}, "display": {"href": "https://publications.scilifelab.se/publication/72e9b85908ed435d8265e945883e4b04"}}, "title": "Alterations of endometrial epithelial-mesenchymal transition and MAPK signalling components in women with PCOS are partially modulated by metformin in vitro.", "authors": [{"family": "Hu", "given": "Min", "initials": "M"}, {"family": "Zhang", "given": "Yuehui", "initials": "Y"}, {"family": "Li", "given": "Xin", "initials": "X"}, {"family": "Cui", "given": "Peng", "initials": "P"}, {"family": "Li", "given": "Juan", "initials": "J"}, {"family": "Br\u00e4nnstr\u00f6m", "given": "Mats", "initials": "M"}, {"family": "Shao", "given": "Linus R", "initials": "LR"}, {"family": "Billig", "given": "H\u00e5kan", "initials": "H"}], "type": "journal article", "published": "2020-05-15", "journal": {"title": "Mol. Hum. Reprod.", "issn": "1460-2407", "volume": "26", "issue": "5", "pages": "312-326", "issn-l": "1360-9947"}, "abstract": "Growing evidence suggests that epithelial-mesenchymal transition (EMT) and its regulator mitogen-activated protein kinase (MAPK) contribute to endometria-related reproductive disorders. However, the regulation of EMT and MAPK signalling components in the endometrium from polycystic ovary syndrome (PCOS) patients has not been systematically investigated and remains elusive. In humans, how metformin induces molecular alterations in the endometrial tissues under PCOS conditions is not completely clear. Here, we recruited 7 non-PCOS patients during the proliferative phase (nPCOS), 7 non-PCOS patients with endometrial hyperplasia (nPCOSEH), 14 PCOS patients during the proliferative phase (PCOS) and 3 PCOS patients with endometrial hyperplasia (PCOSEH). Our studies demonstrated that compared with nPCOS, PCOS patients showed decreased Claudin 1 and increased Vimentin and Slug proteins. Similar to increased Slug protein, nPCOSEH and PCOSEH patients showed increased N-cadherin protein. Western blot and immunostaining revealed increased epithelial phosphorylated Cytokeratin 8 (p-CK 8) expression and an increased p-CK 8:CK 8 ratio in PCOS, nPCOSEH and PCOSEH patients compared to nPCOS patients. Although nPCOSEH and PCOSEH patients showed increased p-ERK1/2 and/or p38 protein levels, the significant increase in p-ERK1/2 expression and p-ERK1/2:ERK1/2 ratio was only found in PCOS patients compared to nPCOS patients. A significant induction of the membrane ER\u03b2 immunostaining was observed in the epithelial cells of PCOS and PCOSEH patients compared to nPCOS and nPCOSEH patients. While in vitro treatment with metformin alone increased Snail and decreased Claudin 1, N-cadherin and \u03b1-SMA proteins, concomitant treatment with metformin and E2 increased the expression of CK 8 and Snail proteins and decreased the expression of Claudin 1, ZO-1, Slug and \u03b1-SMA proteins. Our findings suggest that the EMT contributes to the switch from a healthy state to a PCOS state in the endometrium, which might subsequently drive endometrial injury and dysfunction. We also provide evidence that metformin differentially modulates EMT protein expression in PCOS patients depending on oestrogenic stimulation.", "doi": "10.1093/molehr/gaaa023", "pmid": "32202622", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pii", "key": "5810989"}], "notes": [], "created": "2023-02-16T08:07:26.139Z", "modified": "2023-02-16T08:07:26.154Z"}, {"entity": "publication", "iuid": "e21fd1cb40c740a098c9804b828e3a5c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e21fd1cb40c740a098c9804b828e3a5c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e21fd1cb40c740a098c9804b828e3a5c"}}, "title": "Towards uterus tissue engineering: a comparative study of sheep uterus decellularisation.", "authors": [{"family": "Tiemann", "given": "T T", "initials": "TT"}, {"family": "Padma", "given": "A M", "initials": "AM"}, {"family": "Sehic", "given": "E", "initials": "E"}, {"family": "B\u00e4ckdahl", "given": "H", "initials": "H"}, {"family": "Oltean", "given": "M", "initials": "M"}, {"family": "Song", "given": "M J", "initials": "MJ"}, {"family": "Br\u00e4nnstr\u00f6m", "given": "M", "initials": "M"}, {"family": "Hellstr\u00f6m", "given": "M", "initials": "M"}], "type": "comparative study", "published": "2020-03-26", "journal": {"title": "Mol. Hum. Reprod.", "issn": "1460-2407", "volume": "26", "issue": "3", "pages": "167-178", "issn-l": "1360-9947"}, "abstract": "Uterus tissue engineering may dismantle limitations in current uterus transplantation protocols. A uterine biomaterial populated with patient-derived cells could potentially serve as a graft to circumvent complicated surgery of live donors, immunosuppressive medication and rejection episodes. Repeated uterine bioengineering studies on rodents have shown promising results using decellularised scaffolds to restore fertility in a partially impaired uterus and now mandate experiments on larger and more human-like animal models. The aim of the presented studies was therefore to establish adequate protocols for scaffold generation and prepare for future in vivo sheep uterus bioengineering experiments. Three decellularisation protocols were developed using vascular perfusion through the uterine artery of whole sheep uteri obtained from slaughterhouse material. Decellularisation solutions used were based on 0.5% sodium dodecyl sulphate (Protocol 1) or 2% sodium deoxycholate (Protocol 2) or with a sequential perfusion of 2% sodium deoxycholate and 1% Triton X-100 (Protocol 3). The scaffolds were examined by histology, extracellular matrix quantification, evaluation of mechanical properties and the ability to support foetal sheep stem cells after recellularisation. We showed that a sheep uterus can successfully be decellularised while maintaining a high integrity of the extracellular components. Uteri perfused with sodium deoxycholate (Protocol 2) were the most favourable treatment in our study based on quantifications. However, all scaffolds supported stem cells for 2 weeks in vitro and showed no cytotoxicity signs. Cells continued to express markers for proliferation and maintained their undifferentiated phenotype. Hence, this study reports three valuable decellularisation protocols for future in vivo sheep uterus bioengineering experiments.", "doi": "10.1093/molehr/gaaa009", "pmid": "31980817", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC7103571"}, {"db": "pii", "key": "5715759"}], "notes": [], "created": "2023-02-16T08:09:57.817Z", "modified": "2023-02-16T08:09:57.820Z"}, {"entity": "publication", "iuid": "78c3ad5a0d7e42e5a6a81a16e47c965f", "links": {"self": {"href": "https://publications.scilifelab.se/publication/78c3ad5a0d7e42e5a6a81a16e47c965f.json"}, "display": {"href": "https://publications.scilifelab.se/publication/78c3ad5a0d7e42e5a6a81a16e47c965f"}}, "title": "The human testis-specific proteome defined by transcriptomics and antibody-based profiling.", "authors": [{"family": "Djureinovic", "given": "D", "initials": "D"}, {"family": "Fagerberg", "given": "L", "initials": "L"}, {"family": "Hallstr\u00f6m", "given": "B", "initials": "B"}, {"family": "Danielsson", "given": "A", "initials": "A"}, {"family": "Lindskog", "given": "C", "initials": "C"}, {"family": "Uhl\u00e9n", "given": "M", "initials": "M", "orcid": "0000-0002-4858-8056", "researcher": {"href": "https://publications.scilifelab.se/researcher/ff81da3cb0cf4262873b993a1b06798c.json"}}, {"family": "Pont\u00e9n", "given": "F", "initials": "F"}], "type": "journal article", "published": "2014-06-00", "journal": {"volume": "20", "issn": "1460-2407", "issue": "6", "pages": "476-488", "title": "Mol. Hum. Reprod.", "issn-l": "1360-9947"}, "abstract": "The testis' function is to produce haploid germ cells necessary for reproduction. Here we have combined a genome-wide transcriptomics analysis with immunohistochemistry-based protein profiling to characterize the molecular components of the testis. Deep sequencing (RNA-Seq) of normal human testicular tissue from seven individuals was performed and compared with 26 other normal human tissue types. All 20 050 putative human genes were classified into categories based on expression patterns. The analysis shows that testis is the tissue with the most tissue-specific genes by far. More than 1000 genes show a testis-enriched expression pattern in testis when compared with all other analyzed tissues. Highly testis enriched genes were further characterized with respect to protein localization within the testis, such as spermatogonia, spermatocytes, spermatids, sperm, Sertoli cells and Leydig cells. Here we present an immunohistochemistry-based analysis, showing the localization of corresponding proteins in different cell types and various stages of spermatogenesis, for 62 genes expressed at >50-fold higher levels in testis when compared with other tissues. A large fraction of these genes were unexpectedly expressed in early stages of spermatogenesis. In conclusion, we have applied a genome-wide analysis to identify the human testis-specific proteome using transcriptomics and antibody-based protein profiling, providing lists of genes expressed in a tissue-enriched manner in the testis. The majority of these genes and proteins were previously poorly characterised in terms of localization and function, and our list provides an important starting point to increase our molecular understanding of human reproductive biology and disease.", "doi": "10.1093/molehr/gau018", "pmid": "24598113", "labels": {"National Genomics Infrastructure": null, "Tissue Profiling": null, "NGI Stockholm (Genomics Applications)": null, "NGI Stockholm (Genomics Production)": null}, "xrefs": [{"db": "pii", "key": "gau018"}], "notes": [], "created": "2017-05-04T14:56:08.213Z", "modified": "2021-07-08T13:44:33.374Z"}], "created": "2017-05-09T09:12:11.705Z", "modified": "2020-11-27T13:14:02.955Z"}