{"entity": "journal", "iuid": "c8b40213d1e447e7899f8431bf5031d4", "timestamp": "2026-05-19T00:04:38.998Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Microlife.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Microlife"}}, "title": "Microlife", "issn": "2633-6693", "issn-l": null, "publications_count": 2, "publications": [{"entity": "publication", "iuid": "9645e305fe604802a8e78c37adfec00c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/9645e305fe604802a8e78c37adfec00c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/9645e305fe604802a8e78c37adfec00c"}}, "title": "Functional redundancy revealed by the deletion of the mimivirus GMC-oxidoreductase genes.", "authors": [{"family": "Alempic", "given": "Jean-Marie", "initials": "JM"}, {"family": "Bisio", "given": "Hugo", "initials": "H"}, {"family": "Villalta", "given": "Alejandro", "initials": "A"}, {"family": "Santini", "given": "S\u00e9bastien", "initials": "S"}, {"family": "Lartigue", "given": "Audrey", "initials": "A"}, {"family": "Schmitt", "given": "Alain", "initials": "A"}, {"family": "Bugnot", "given": "Claire", "initials": "C"}, {"family": "Notaro", "given": "Anna", "initials": "A"}, {"family": "Belmudes", "given": "Lucid", "initials": "L"}, {"family": "Adrait", "given": "Annie", "initials": "A"}, {"family": "Poirot", "given": "Olivier", "initials": "O"}, {"family": "Ptchelkine", "given": "Denis", "initials": "D"}, {"family": "De Castro", "given": "Cristina", "initials": "C", "orcid": "0000-0002-5147-1756", "researcher": {"href": "https://publications.scilifelab.se/researcher/17503639cb344f02aa15d654195ba0cd.json"}}, {"family": "Cout\u00e9", "given": "Yohann", "initials": "Y"}, {"family": "Abergel", "given": "Chantal", "initials": "C", "orcid": "0000-0003-1875-4049", "researcher": {"href": "https://publications.scilifelab.se/researcher/da2c09812e3e451fbfb73da3dd8db7fc.json"}}], "type": "journal article", "published": "2024-04-05", "journal": {"title": "Microlife", "issn": "2633-6693", "volume": "5", "pages": "uqae006", "issn-l": null}, "abstract": "The mimivirus 1.2 Mb genome was shown to be organized into a nucleocapsid-like genomic fiber encased in the nucleoid compartment inside the icosahedral capsid. The genomic fiber protein shell is composed of a mixture of two GMC-oxidoreductase paralogs, one of them being the main component of the glycosylated layer of fibrils at the surface of the virion. In this study, we determined the effect of the deletion of each of the corresponding genes on the genomic fiber and the layer of surface fibrils. First, we deleted the GMC-oxidoreductase, the most abundant in the genomic fiber, and determined its structure and composition in the mutant. As expected, it was composed of the second GMC-oxidoreductase and contained 5- and 6-start helices similar to the wild-type fiber. This result led us to propose a model explaining their coexistence. Then we deleted the GMC-oxidoreductase, the most abundant in the layer of fibrils, to analyze its protein composition in the mutant. Second, we showed that the fitness of single mutants and the double mutant were not decreased compared with the wild-type viruses under laboratory conditions. Third, we determined that deleting the GMC-oxidoreductase genes did not impact the glycosylation or the glycan composition of the layer of surface fibrils, despite modifying their protein composition. Because the glycosylation machinery and glycan composition of members of different clades are different, we expanded the analysis of the protein composition of the layer of fibrils to members of the B and C clades and showed that it was different among the three clades and even among isolates within the same clade. Taken together, the results obtained on two distinct central processes (genome packaging and virion coating) illustrate an unexpected functional redundancy in members of the family Mimiviridae, suggesting this may be the major evolutionary force behind their giant genomes.", "doi": "10.1093/femsml/uqae006", "pmid": "38659623", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC11042495"}, {"db": "pii", "key": "uqae006"}], "notes": [], "created": "2024-07-17T18:48:20.634Z", "modified": "2024-11-15T13:46:14.622Z"}, {"entity": "publication", "iuid": "7f561a7a56c645f9970495ae20a27a3c", "links": {"self": {"href": "https://publications.scilifelab.se/publication/7f561a7a56c645f9970495ae20a27a3c.json"}, "display": {"href": "https://publications.scilifelab.se/publication/7f561a7a56c645f9970495ae20a27a3c"}}, "title": "Apilactobacillus kunkeei releases RNA-associated membrane vesicles and proteinaceous nanoparticles.", "authors": [{"family": "Seeger", "given": "Christian", "initials": "C"}, {"family": "Dyrhage", "given": "Karl", "initials": "K"}, {"family": "N\u00e4slund", "given": "Kristina", "initials": "K"}, {"family": "Andersson", "given": "Siv G E", "initials": "SGE", "orcid": "0000-0003-0864-0259", "researcher": {"href": "https://publications.scilifelab.se/researcher/7884a5058383428ea3cbe7fc4505f36a.json"}}], "type": "journal article", "published": "2023-08-29", "journal": {"title": "Microlife", "issn": "2633-6693", "volume": "4", "pages": "uqad037", "issn-l": null}, "abstract": "Extracellularly released particles, including membrane vesicles, have increasingly been recognized as important for bacterial community functions and host-interaction processes, but their compositions and functional roles differ between species and also between strains of the same species. In this study, we have determined the composition of membrane vesicles and protein particles identified in the cell-free pellets of two strains of Apilactobacillus kunkeei, a defensive symbiont of honeybees. The membrane vesicles were separated from the extracellular particles using density gradient ultracentrifugation. The peaks of the RNA and protein distributions were separated from each other and the highest concentration of RNA was observed in the fractions that contained the membrane vesicles while the highest protein concentration coincided with the fractions that contained extracellular particles. A comparative proteomics analysis by LC-MS/MS showed that 37 proteins with type-I signal peptides were consistently identified across the fractionated samples obtained from the cell-free pellets, of which 29 were orthologs detected in both strains. Functional predictions of the extracellular proteins revealed the presence of glycoside hydrolases, glycosyltransferases, giant proteins and peptidases. The extracellular transcriptomes mapped to a broad set of genes with a similar functional profile as the whole cell transcriptome. This study provides insights into the composition of membrane vesicles and extracellular proteins of a bee-associated symbiont.", "doi": "10.1093/femsml/uqad037", "pmid": "37705871", "labels": {"Cryo-EM": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC10496945"}, {"db": "pii", "key": "uqad037"}], "notes": [], "created": "2023-09-19T08:21:55.835Z", "modified": "2023-09-19T08:21:55.988Z"}], "created": "2023-09-19T08:21:55.962Z", "modified": "2023-09-19T08:21:55.962Z"}