{"entity": "journal", "iuid": "51f625938c6d48a5872ee15468c6216b", "timestamp": "2026-08-07T18:56:59.273Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Microb%20Biotechnol.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Microb%20Biotechnol"}}, "title": "Microb Biotechnol", "issn": "1751-7915", "issn-l": "1751-7915", "publications_count": 4, "publications": [{"entity": "publication", "iuid": "d05046c85a1e4f1ca7defccbb1420945", "links": {"self": {"href": "https://publications.scilifelab.se/publication/d05046c85a1e4f1ca7defccbb1420945.json"}, "display": {"href": "https://publications.scilifelab.se/publication/d05046c85a1e4f1ca7defccbb1420945"}}, "title": "Direct effects of organic pollutants on the growth and gene expression of the Baltic Sea model bacterium Rheinheimera sp. BAL341.", "authors": [{"family": "Karlsson", "given": "Christofer M G", "initials": "CMG"}, {"family": "Cerro-G\u00e1lvez", "given": "Elena", "initials": "E"}, {"family": "Lundin", "given": "Daniel", "initials": "D"}, {"family": "Karlsson", "given": "Camilla", "initials": "C"}, {"family": "Vila-Costa", "given": "Maria", "initials": "M"}, {"family": "Pinhassi", "given": "Jarone", "initials": "J"}], "type": "journal article", "published": "2019-09-00", "journal": {"volume": "12", "issn": "1751-7915", "issue": "5", "pages": "892-906", "title": "Microb Biotechnol", "issn-l": "1751-7915"}, "abstract": "Organic pollutants (OPs) are critically toxic, bioaccumulative and globally widespread. Moreover, several OPs negatively influence aquatic wildlife. Although bacteria are major drivers of the ocean carbon cycle and the turnover of vital elements, there is limited knowledge of OP effects on heterotrophic bacterioplankton. We therefore investigated growth and gene expression responses of the Baltic Sea model bacterium Rheinheimera sp. BAL341 to environmentally relevant concentrations of distinct classes of OPs in 2-h incubation experiments. During exponential growth, exposure to a mix of polycyclic aromatic hydrocarbons, alkanes and organophosphate esters (denoted MIX) resulted in a significant decrease (between 9% and 18%) in bacterial abundance and production compared with controls. In contrast, combined exposure to perfluorooctanesulfonic acids and perfluorooctanoic acids (denoted PFAS) had no significant effect on growth. Nevertheless, MIX and PFAS exposures both induced significant shifts in gene expression profiles compared with controls in exponential growth. This involved several functional metabolism categories (e.g. stress response and fatty acids metabolism), some of which were pollutant-specific (e.g. phosphate acquisition and alkane-1 monooxygenase genes). In stationary phase, only two genes in the MIX treatment were significantly differentially expressed. The substantial direct influence of OPs on metabolism during bacterial growth suggests that widespread OPs could severely alter biogeochemical processes governed by bacterioplankton.", "doi": "10.1111/1751-7915.13441", "pmid": "31270938", "labels": {"National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Applications)": "Service", "NGI Stockholm (Genomics Production)": "Service", "Bioinformatics Support for Computational Resources": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC6680617"}, {"db": "GENBANK", "key": "KM586890"}], "notes": [], "created": "2019-12-02T16:52:24.485Z", "modified": "2024-01-16T13:48:43.965Z"}, {"entity": "publication", "iuid": "30254f53a35b4096a4bc05bfa00bcbeb", "links": {"self": {"href": "https://publications.scilifelab.se/publication/30254f53a35b4096a4bc05bfa00bcbeb.json"}, "display": {"href": "https://publications.scilifelab.se/publication/30254f53a35b4096a4bc05bfa00bcbeb"}}, "title": "Detection of novel syntrophic acetate-oxidizing bacteria from biogas processes by continuous acetate enrichment approaches.", "authors": [{"family": "Westerholm", "given": "Maria", "initials": "M"}, {"family": "M\u00fcller", "given": "Bettina", "initials": "B"}, {"family": "Singh", "given": "Abhijeet", "initials": "A"}, {"family": "Karlsson Lindsj\u00f6", "given": "Oskar", "initials": "O"}, {"family": "Schn\u00fcrer", "given": "Anna", "initials": "A"}], "type": "journal article", "published": "2018-07-00", "journal": {"volume": "11", "issn": "1751-7915", "issue": "4", "pages": "680-693", "title": "Microb Biotechnol", "issn-l": "1751-7915"}, "abstract": "To enrich syntrophic acetate-oxidizing bacteria (SAOB), duplicate chemostats were inoculated with sludge from syntrophic acetate oxidation (SAO)-dominated systems and continuously supplied with acetate (0.4 or 7.5\u00a0g\u00a0l", "doi": "10.1111/1751-7915.13035", "pmid": "29239113", "labels": {"National Genomics Infrastructure": "Service", "NGI Stockholm (Genomics Applications)": "Service", "NGI Stockholm (Genomics Production)": "Service"}, "xrefs": [{"db": "pmc", "key": "PMC6011928"}, {"db": "GENBANK", "key": "KJ701175"}, {"db": "GENBANK", "key": "MG356789"}, {"db": "GENBANK", "key": "MG356790"}, {"db": "GENBANK", "key": "MG012806"}, {"db": "GENBANK", "key": "MG012840"}, {"db": "GENBANK", "key": "MG385064"}, {"db": "GENBANK", "key": "MG385074"}], "notes": [], "created": "2018-10-31T19:48:31.526Z", "modified": "2020-01-21T13:56:10.989Z"}, {"entity": "publication", "iuid": "812e665e561c421f97d2887a450329ab", "links": {"self": {"href": "https://publications.scilifelab.se/publication/812e665e561c421f97d2887a450329ab.json"}, "display": {"href": "https://publications.scilifelab.se/publication/812e665e561c421f97d2887a450329ab"}}, "title": "Strain improvement of Pichia kudriavzevii TY13 for raised phytase production and reduced phosphate repression.", "authors": [{"family": "Qvirist", "given": "Linnea", "initials": "L"}, {"family": "Vorontsov", "given": "Egor", "initials": "E"}, {"family": "Veide Vilg", "given": "Jenny", "initials": "J"}, {"family": "Andlid", "given": "Thomas", "initials": "T"}], "type": "journal article", "published": "2017-03-00", "journal": {"title": "Microb Biotechnol", "issn": "1751-7915", "volume": "10", "issue": "2", "pages": "341-353", "issn-l": "1751-7915"}, "abstract": "In this work, we present the development and characterization of a strain of Pichia kudriavzevii (TY1322), with highly improved phytate-degrading capacity. The mutant strain TY1322 shows a biomass-specific phytate degradation of 1.26 mmol g -1 h-1 after 8 h of cultivation in a high-phosphate medium, which is about 8 times higher compared with the wild-type strain. Strain TY1322 was able to grow at low pH (pH 2), at high temperature (46\u00b0C) and in the presence of ox bile (2% w/v), indicating this strain's ability to survive passage through the gastrointestinal tract. The purified phytase showed two pH optima, at pH 3.5 and 5.5, and one temperature optimum at 55\u00b0C. The lower pH optimum of 3.5 matches the reported pH of the pig stomach, meaning that TY1322 and/or its phytase is highly suitable for use in feed production. Furthermore, P. kudriavzevii TY1322 tolerates ethanol up to 6% (v/v) and shows high osmotic stress tolerance. Owing to the phenotypic characteristics and non-genetically modified organisms nature of TY1322, this strain show great potential for future uses in (i) cereal fermentations for increased mineral bioavailability, and (ii) feed production to increase the phosphate bioavailability for monogastric animals to reduce the need for artificial phosphate fortification.", "doi": "10.1111/1751-7915.12427", "pmid": "27790831", "labels": {"Glycoproteomics and MS Proteomics": "Collaborative"}, "xrefs": [{"db": "pmc", "key": "PMC5328827"}], "notes": [], "created": "2020-01-30T15:58:19.867Z", "modified": "2024-01-16T13:46:32.776Z"}, {"entity": "publication", "iuid": "97352b4103e94523b7562e842be4a358", "links": {"self": {"href": "https://publications.scilifelab.se/publication/97352b4103e94523b7562e842be4a358.json"}, "display": {"href": "https://publications.scilifelab.se/publication/97352b4103e94523b7562e842be4a358"}}, "title": "Revolutionizing membrane protein overexpression in bacteria.", "authors": [{"family": "Schlegel", "given": "Susan", "initials": "S"}, {"family": "Klepsch", "given": "Mirjam", "initials": "M"}, {"family": "Gialama", "given": "Dimitra", "initials": "D"}, {"family": "Wickstr\u00f6m", "given": "David", "initials": "D"}, {"family": "Slotboom", "given": "Dirk Jan", "initials": "DJ"}, {"family": "de Gier", "given": "Jan-Willem", "initials": "JW"}], "type": "journal article", "published": "2010-07-00", "journal": {"volume": "3", "issn": "1751-7915", "issue": "4", "pages": "403-411", "title": "Microb Biotechnol", "issn-l": "1751-7915"}, "abstract": "The bacterium Escherichia coli is the most widely used expression host for overexpression trials of membrane proteins. Usually, different strains, culture conditions and expression regimes are screened for to identify the optimal overexpression strategy. However, yields are often not satisfactory, especially for eukaryotic membrane proteins. This has initiated a revolution of membrane protein overexpression in bacteria. Recent studies have shown that it is feasible to (i) engineer or select for E. coli strains with strongly improved membrane protein overexpression characteristics, (ii) use bacteria other than E. coli for the expression of membrane proteins, (iii) engineer or select for membrane protein variants that retain functionality but express better than the wild-type protein, and (iv) express membrane proteins using E. coli-based cell-free systems.", "doi": "10.1111/j.1751-7915.2009.00148.x", "pmid": "21255339", "labels": {"National Genomics Infrastructure": null, "NGI Stockholm (Genomics Applications)": null, "NGI Stockholm (Genomics Production)": null}, "xrefs": [{"db": "pmc", "key": "PMC3815807"}], "notes": [], "created": "2017-05-04T14:57:12.269Z", "modified": "2020-01-21T13:56:03.893Z"}], "created": "2017-05-09T09:12:30.529Z", "modified": "2020-11-27T13:14:06.829Z"}