{"entity": "journal", "iuid": "1d5c8bc04e7e4e2293883f7c9cabbdf1", "timestamp": "2026-08-17T01:20:11.466Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/Lab%20Chip.json"}, "display": {"href": "https://publications.scilifelab.se/journal/Lab%20Chip"}}, "title": "Lab Chip", "issn": "1473-0197", "issn-l": "1473-0189", "publications_count": 3, "publications": [{"entity": "publication", "iuid": "8a944c22f1ab482e8a31f8af3b240aa9", "links": {"self": {"href": "https://publications.scilifelab.se/publication/8a944c22f1ab482e8a31f8af3b240aa9.json"}, "display": {"href": "https://publications.scilifelab.se/publication/8a944c22f1ab482e8a31f8af3b240aa9"}}, "title": "An in vivo mimetic liver-lobule-chip (LLoC) for stem cell maturation, and zonation of hepatocyte-like cells on chip.", "authors": [{"family": "Dalsbecker", "given": "Philip", "initials": "P", "orcid": "0000-0002-9762-2935", "researcher": {"href": "https://publications.scilifelab.se/researcher/f9f86bdd978e4ac399fa9da18dba97ae.json"}}, {"family": "Suominen", "given": "Siiri", "initials": "S"}, {"family": "Faridi", "given": "Muhammad Asim", "initials": "MA"}, {"family": "Mahdavi", "given": "Reza", "initials": "R"}, {"family": "Johansson", "given": "Julia", "initials": "J"}, {"family": "Blomqvist", "given": "Charlotte Hamngren", "initials": "CH"}, {"family": "Goks\u00f6r", "given": "Mattias", "initials": "M"}, {"family": "Aalto-Set\u00e4l\u00e4", "given": "Katriina", "initials": "K"}, {"family": "Viiri", "given": "Leena E", "initials": "LE"}, {"family": "Adiels", "given": "Caroline B", "initials": "CB", "orcid": "0000-0002-2403-2213", "researcher": {"href": "https://publications.scilifelab.se/researcher/029f8a8f2f704d358b8743d821c03bd7.json"}}], "type": "journal article", "published": "2025-08-19", "journal": {"title": "Lab Chip", "issn": "1473-0189", "volume": "25", "issue": "17", "pages": "4328-4344", "issn-l": null}, "abstract": "In vitro cell culture models play a crucial role in preclinical drug discovery. To achieve optimal culturing environments and establish physiologically relevant organ-specific conditions, it is imperative to replicate in vivo scenarios when working with primary or induced pluripotent cell types. However, current approaches to recreating in vivo conditions and generating relevant 3D cell cultures still fall short. In this study, we validate a liver-lobule-chip (LLoC) containing 21 artificial liver lobules, each representing the smallest functional unit of the human liver. The LLoC facilitates diffusion-based perfusion via sinusoid-mimetic structures, providing physiologically relevant shear stress exposure and radial nutrient concentration gradients within each lobule. We demonstrate the feasibility of long term cultures (up to 14 days) of viable and functional HepG2 cells in a 3D discoid tissue structure, serving as initial proof of concept. Thereafter, we successfully differentiate sensitive, human induced pluripotent stem cell (iPSC)-derived cells into hepatocyte-like cells over a period of 20 days on-chip, exhibiting advancements in maturity compared to traditional 2D cultures. Further, hepatocyte-like cells cultured in the LLoC exhibit zonated protein expression profiles, indicating the presence of metabolic gradients characteristic of liver lobules. Our results highlight the suitability of the LLoC for long-term discoid tissue cultures, specifically for iPSCs, and their differentiation in a perfused environment. We envision the LLoC as a starting point for more advanced in vitro models, allowing for the combination of multiple liver cell types to create a comprehensive liver model for disease-onchip studies. Ultimately, when combined with stem cell technology, the LLoC offers a promising and robust on-chip liver model that serves as a viable alternative to primary hepatocyte cultures-ideally suited for preclinical drug screening and personalized medicine applications.", "doi": "10.1039/d4lc00509k", "pmid": "40485317", "labels": {"Integrated Microscopy Technologies Gothenburg": "Service"}, "xrefs": [], "notes": [], "created": "2025-11-05T13:48:17.697Z", "modified": "2025-11-05T13:48:17.823Z"}, {"entity": "publication", "iuid": "34f2371179704c95b05bc42435907de7", "links": {"self": {"href": "https://publications.scilifelab.se/publication/34f2371179704c95b05bc42435907de7.json"}, "display": {"href": "https://publications.scilifelab.se/publication/34f2371179704c95b05bc42435907de7"}}, "title": "Thiol-ene-epoxy thermoset for low-temperature bonding to biofunctionalized microarray surfaces.", "authors": [{"family": "Zhou", "given": "Xiamo C", "initials": "XC"}, {"family": "Sj\u00f6berg", "given": "Ronald", "initials": "R", "orcid": "0000-0003-1363-5796", "researcher": {"href": "https://publications.scilifelab.se/researcher/d08326da26da422ab445a26563843e79.json"}}, {"family": "Druet", "given": "Amaury", "initials": "A"}, {"family": "Schwenk", "given": "Jochen M", "initials": "JM", "orcid": "0000-0001-8141-8449", "researcher": {"href": "https://publications.scilifelab.se/researcher/aba5822711b246b397fffacb7ae403b3.json"}}, {"family": "van der Wijngaart", "given": "Wouter", "initials": "W"}, {"family": "Haraldsson", "given": "Tommy", "initials": "T"}, {"family": "Carlborg", "given": "Carl Fredrik", "initials": "CF"}], "type": "journal article", "published": "2017-10-25", "journal": {"volume": "17", "issn": "1473-0189", "issue": "21", "pages": "3672-3681", "title": "Lab Chip", "issn-l": null}, "abstract": "One way to improve the sensitivity and throughput of miniaturized biomolecular assays is to integrate microfluidics to enhance the transport efficiency of biomolecules to the reaction sites. Such microfluidic integration requires bonding of a prefabricated microfluidic gasket to an assay surface without destroying its biological activity. In this paper we address the largely unmet challenge to accomplish a proper seal between a microfluidic gasket and a protein surface, with maintained biological activity and without contaminating the surface or blocking the microfluidic channels. We introduce a novel dual cure polymer resin for the formation of microfluidic gaskets that can be room-temperature bonded to a range of substrates using only UVA light. This polymer is the first polymer that features over a month of shelf life between the structure formation and the bonding, moreover the fully cured polymer gaskets feature the following set of properties suitable for microfluidics: high stiffness, which prevents microfluidic channel collapse during handling; very limited absorption of biomolecules; and no significant leaching of uncured monomers. We describe the novel polymer resin and its characteristics, study through FT-IR, and demonstrate its use as microfluidic well-arrays bonded onto protein array slides at room temperature followed by multiplexed immunoassays. The results confirm maintained biological activity and show high repeatability between protein arrays. This new approach for integrating microfluidic gaskets to biofunctionalised surfaces has the potential to improve sample throughput and decrease manufacturing costs for miniaturized biomolecular systems.", "doi": "10.1039/c7lc00652g", "pmid": "28975170", "labels": {"Autoimmunity and Serology Profiling": "Technology development", "Affinity Proteomics Stockholm": "Technology development"}, "xrefs": [], "notes": [], "created": "2017-11-02T12:13:55.170Z", "modified": "2021-07-08T12:07:34.033Z"}, {"entity": "publication", "iuid": "77013ba616c2495e8f68bf6a247e26f2", "links": {"self": {"href": "https://publications.scilifelab.se/publication/77013ba616c2495e8f68bf6a247e26f2.json"}, "display": {"href": "https://publications.scilifelab.se/publication/77013ba616c2495e8f68bf6a247e26f2"}}, "title": "Towards encoded particles for highly multiplexed colorimetric point of care autoantibody detection.", "authors": [{"family": "Svedberg", "given": "Gustav", "initials": "G"}, {"family": "Jeong", "given": "Yunjin", "initials": "Y"}, {"family": "Na", "given": "Hunjong", "initials": "H"}, {"family": "Jang", "given": "Jisung", "initials": "J"}, {"family": "Nilsson", "given": "Peter", "initials": "P", "orcid": "0000-0002-4657-8532", "researcher": {"href": "https://publications.scilifelab.se/researcher/799bcf1cf8cf451296f4535dd4ca9dc0.json"}}, {"family": "Kwon", "given": "Sunghoon", "initials": "S"}, {"family": "Gantelius", "given": "Jesper", "initials": "J"}, {"family": "Svahn", "given": "Helene Andersson", "initials": "HA"}], "type": "journal article", "published": "2017-01-31", "journal": {"volume": "17", "issn": "1473-0189", "issue": "3", "pages": "549-556", "title": "Lab Chip", "issn-l": null}, "abstract": "Highly multiplexed point of care tests could improve diagnostic accuracy and differential diagnostic capacity in for instance emergency medicine and low resource environments. Available technology platforms for POC biomarker detection are typically simplex or low-plexed, whereas common lab-based microarray systems allow for the simultaneous detection of thousands of DNA or protein biomarkers. In this study, we demonstrate a novel suspension particle array platform that utilizes 900 \u03bcm bricks for POC amenable colorimetric biomarker detection with an encoding capacity of over two million. Due to the mm-scale size, both the lithographic codes and colorimetric signals of individual particles can be visualized using a consumer grade office flatbed scanner, with a potential for simultaneous imaging of around 19\u2009000 particles per scan. The analytical sensitivity of the assay was determined to be 4 ng ml(-1) using an antibody model system. As a proof of concept, autoantibodies toward anoctamin 2 were detected in order to discriminate between multiple sclerosis plasma samples and healthy controls with p < 0.0001 and an inter-assay % CV of 9.44%.", "doi": "10.1039/c6lc01358a", "pmid": "28102419", "labels": {"Autoimmunity and Serology Profiling": "Service"}, "xrefs": [], "notes": [], "created": "2017-11-02T11:41:16.257Z", "modified": "2021-07-07T15:50:03.198Z"}], "created": "2017-11-02T11:41:16.276Z", "modified": "2020-11-27T13:14:03.799Z"}