{"entity": "journal", "iuid": "e0f196066942423aa29493302c8707ec", "timestamp": "2026-06-18T14:10:19.879Z", "links": {"self": {"href": "https://publications.scilifelab.se/journal/J%20Insect%20Physiol.json"}, "display": {"href": "https://publications.scilifelab.se/journal/J%20Insect%20Physiol"}}, "title": "J Insect Physiol", "issn": "1879-1611", "issn-l": null, "publications_count": 2, "publications": [{"entity": "publication", "iuid": "5f2f890616854701b6931417bb2c2efd", "links": {"self": {"href": "https://publications.scilifelab.se/publication/5f2f890616854701b6931417bb2c2efd.json"}, "display": {"href": "https://publications.scilifelab.se/publication/5f2f890616854701b6931417bb2c2efd"}}, "title": "Metabolites, ions, and the mechanisms behind seasonal cold hardening of Pyrochroa coccinea (Pyrochroidae) larvae.", "authors": [{"family": "Jedal", "given": "Jonathan Y B", "initials": "JYB"}, {"family": "Malmendal", "given": "Anders", "initials": "A"}, {"family": "Raml\u00f8v", "given": "Hans", "initials": "H"}], "type": "journal article", "published": "2024-03-00", "journal": {"title": "J Insect Physiol", "issn": "1879-1611", "volume": "153", "pages": "104610", "issn-l": null}, "abstract": "The larvae of the black headed cardinal beetle Pyrochroa coccinea, overwinters under the bark of dead logs in northern European dioecious forests, and are thus exposed to temperatures below the melting point of their bodily fluids. Here we explore the mechanisms behind their seasonal cold hardening by characterising field samples collected monthly throughout the year. Both the lower lethal temperature and supercooling point dropped as much as 10\u2103 in the second half of November, reaching values around -15\u2103 by the beginning of December. This change was accompanied by a 320 mosmol/kg increase in hemolymph osmolality, which is a doubling compared to the summer levels. We used NMR metabolomics to identify and measure the absolute concentrations of the responsible cryoprotective C-H containing metabolites in the hemolymph. The largest increase was found to be in either glucose or trehalose, with an average total increase of 120 mM. Proline, alanine, and choline concentrations were found to increase by around 10 mM each. Contrarily, phosphocholine and phosphoethanolamine were halved, resulting in a total decrease of around 50 mM. These measurements were complemented with ion exchange chromatography measurements. This allowed us to account for all the osmotic pressure in the summer hemolymph, and the measured concentration changes explained as much as 40 % of the observed osmolality increase upon cold hardening. Preliminary results indicate that the remainder may be explained by non-colligative protein contributions.", "doi": "10.1016/j.jinsphys.2023.104610", "pmid": "38145824", "labels": {"Swedish NMR Centre": "Service"}, "xrefs": [{"db": "pii", "key": "S0022-1910(23)00136-1"}], "notes": [], "created": "2024-01-15T09:21:33.833Z", "modified": "2025-10-17T13:03:53.105Z"}, {"entity": "publication", "iuid": "e080ac8b92d94141a21bcb62c7972067", "links": {"self": {"href": "https://publications.scilifelab.se/publication/e080ac8b92d94141a21bcb62c7972067.json"}, "display": {"href": "https://publications.scilifelab.se/publication/e080ac8b92d94141a21bcb62c7972067"}}, "title": "Metabolomics reveals changes in metabolite profiles due to growth and metamorphosis during the ontogeny of the northern damselfly.", "authors": [{"family": "Sp\u00e4th", "given": "Jana", "initials": "J"}, {"family": "Brodin", "given": "Tomas", "initials": "T"}, {"family": "McCallum", "given": "Erin", "initials": "E"}, {"family": "Cerveny", "given": "Daniel", "initials": "D"}, {"family": "Fick", "given": "Jerker", "initials": "J"}, {"family": "Nording", "given": "Malin L", "initials": "ML"}], "type": "journal article", "published": "2022-01-00", "journal": {"title": "J Insect Physiol", "issn": "1879-1611", "volume": "136", "pages": "104341", "issn-l": null}, "abstract": "Many insects have complex life cycles where a drastic ontogenetic change happens between the larval stages and the adult stage, i.e. metamorphosis. Damselflies (order Odonata, suborder Zygoptera) are widely distributed and ecologically important semi-aquatic insects with a complex life cycle. Phenotypic changes over damselfly ontogeny have been documented, however, if and how metabolite profiles are also changing is currently unknown. Here we used a metabolomics methodology to gain insights into the metabolic changes during the life cycle of the Northern damselfly (Coenagrion hastulatum). Hatchlings of wild-caught damselflies were reared in the laboratory and metabolomics analyses using liquid chromatography and gas chromatography coupled to mass spectrometry were carried out at three larval stages and on adult damselflies. Additionally, a subset of larvae was exposed to wastewater effluent to assess how metabolite profiles responded to an environmental stressor. A total of 212 compounds belonging to several classes (e.g. amino acids, fatty acids, sugars) were annotated. Across metamorphosis, we found that damselflies shifted from protein catabolism to lipid catabolism. Wastewater effluent exposure resulted in ontogenetic stage-dependent changes of individual metabolites, but not to a marked extent. Overall, our study is one of the first to describe changes of metabolite profiles during ontogeny of an insect, and it provides a first step towards a greater understanding of the physiological changes occurring during general insect-but especially damselfly-ontogeny.", "doi": "10.1016/j.jinsphys.2021.104341", "pmid": "34843740", "labels": {"Swedish Metabolomics Centre": "Service"}, "xrefs": [{"db": "pii", "key": "S0022-1910(21)00151-7"}], "notes": [], "created": "2021-12-09T20:05:51.586Z", "modified": "2025-10-17T13:03:15.464Z"}], "created": "2021-12-09T20:05:51.610Z", "modified": "2021-12-09T20:05:51.611Z"}